BioVector? CW11033 人誘導(dǎo)多能干細(xì)胞系 / Human Induced Pluripotent Stem Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? CW11033 細(xì)胞
同義詞: BioVector? CIRMi12J-A
中文名稱: BioVector? CW11033 人誘導(dǎo)多能干細(xì)胞系
英文名稱: BioVector? CW11033 Human Induced Pluripotent Stem Cell Line
產(chǎn)品類別: 誘導(dǎo)多能干細(xì)胞(iPSC)系
物種來源: 人 (Homo sapiens)
組織來源: 外周血
細(xì)胞類型: T淋巴細(xì)胞來源的誘導(dǎo)多能干細(xì)胞
患者信息: 55歲女性；非裔美國人
疾病: 單相抑郁癥
構(gòu)建方法: 采用附加型載體(非整合型)重編程技術(shù)，從外周血T淋巴細(xì)胞建立

構(gòu)建機(jī)構(gòu): 美國加州再生醫(yī)學(xué)研究所
形態(tài)特性: 胚胎干細(xì)胞樣形態(tài)，形成緊密的克隆集落，邊界清晰
生長特性: 貼壁生長，依賴飼養(yǎng)層細(xì)胞或無飼養(yǎng)層培養(yǎng)體系
特性特征:

1. 多能性標(biāo)志物: 表達(dá)多能性標(biāo)志物OCT4、NANOG、LIN28、SSEA-4等

2. 分化潛能: 具有向三個(gè)胚層分化的潛能

3. 核型: 正常女性核型(46, XX)

4. 病毒檢測: HIV-1、HIV-2、乙肝病毒、丙肝病毒檢測均為陰性；支原體檢測陰性

5. 研究應(yīng)用: 疾病模型研究(抑郁癥)、藥物篩選、發(fā)育生物學(xué)研究、細(xì)胞治療研究
   培養(yǎng)基: BioVector? CW11033 細(xì)胞專用完全培養(yǎng)基(推薦基礎(chǔ)成分：Essential 8培養(yǎng)基或StemFit AK02N培養(yǎng)基)
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 當(dāng)細(xì)胞集落達(dá)到適宜密度時(shí)，推薦使用 BioVector? 專用傳代試劑(如EDTA或分散酶)進(jìn)行消化，按適宜比例進(jìn)行傳代
   凍存液: BioVector? 干細(xì)胞專用凍存液
   質(zhì)量控制: 支原體檢測：陰性；無菌檢測：陰性；多能性標(biāo)志物表達(dá)：陽性；核型分析：正常
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存(-150°C至-190°C)
   生物安全等級: 1
   僅供科研使用

English:
BioVector? CW11033 Human Induced Pluripotent Stem Cell Line Datasheet

Product Name: BioVector? CW11033 Cells
Synonyms: BioVector? CIRMi12J-A
Chinese Name: BioVector? CW11033 人誘導(dǎo)多能干細(xì)胞系
English Name: BioVector? CW11033 Human Induced Pluripotent Stem Cell Line
Product Category: Induced Pluripotent Stem Cell (iPSC) Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Peripheral blood
Cell Type: T-lymphocyte-derived induced pluripotent stem cells
Patient Information: 55-year-old female; African American
Disease: Unipolar depression
Derivation: Established from peripheral blood T-lymphocytes using episomal vector (non-integrating) reprogramming technology
Establishing Institution: California Institute for Regenerative Medicine, USA
Morphology: Embryonic stem cell-like morphology, forming tight colonies with clear boundaries
Growth Characteristics: Adherent growth, dependent on feeder cells or feeder-free culture systems
Characteristics:

1. Pluripotency Markers: Expresses pluripotency markers OCT4, NANOG, LIN28, SSEA-4, etc.

2. Differentiation Potential: Capable of differentiating into three germ layers

3. Karyotype: Normal female karyotype (46, XX)

4. Virus Testing: Negative for HIV-1, HIV-2, Hepatitis B virus, Hepatitis C virus; Mycoplasma negative

5. Research Applications: Disease modeling (depression), drug screening, developmental biology research, cell therapy research
   Culture Medium: BioVector? Complete Medium for CW11033 Cells (Recommended composition: Essential 8 medium or StemFit AK02N medium)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: When colonies reach appropriate density, recommended to use BioVector? Subculture Reagent (e.g., EDTA or dispase). Subculture at an appropriate split ratio
   Freeze Medium: BioVector? Stem Cell Freeze Medium
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Pluripotency Marker Expression: Positive; Karyotype Analysis: Normal
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1
   For Research Use Only

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