BioVector? MD901 人彌漫大B細(xì)胞淋巴瘤細(xì)胞系 / Human Diffuse Large B-cell Lymphoma Cell Line 數(shù)據(jù)說(shuō)明書(shū)

產(chǎn)品名稱: BioVector? MD901 細(xì)胞
同義詞: BioVector? MD-901
中文名稱: BioVector? MD901 人彌漫性大B細(xì)胞淋巴瘤細(xì)胞系
英文名稱: BioVector? MD901 Human Diffuse Large B-cell Lymphoma Cell Line
產(chǎn)品類(lèi)別: 血液腫瘤細(xì)胞系
物種來(lái)源: 人 (Homo sapiens)
組織來(lái)源: 不確定，可能為外周血或淋巴結(jié)
疾病: 彌漫性大B細(xì)胞淋巴瘤 (DLBCL)
患者信息: 未明確報(bào)道，基于細(xì)胞系建立時(shí)間推測(cè)為成人
形態(tài)特性: 懸浮生長(zhǎng)，淋巴母細(xì)胞樣
生長(zhǎng)特性: 懸浮生長(zhǎng)
構(gòu)建方法: 從彌漫性大B細(xì)胞淋巴瘤患者樣本中建立
分子特征:

1. 腫瘤亞型: 屬于活化B細(xì)胞樣（ABC）亞型

2. EB病毒狀態(tài): 未報(bào)道，通常DLBCL細(xì)胞系多為EBV陰性

3. CREBBP狀態(tài): CREBBP基因野生型，常用于CREBBP功能缺失研究

4. 基因表達(dá)特征: bcl-6 mRNA表達(dá)較強(qiáng)（涉及Ig基因易位）；miR-28有表達(dá)；LPP mRNA表達(dá)陽(yáng)性

5. 蛋白表達(dá): BCL-6蛋白表達(dá)水平較高；LPP蛋白表達(dá)陽(yáng)性
   研究應(yīng)用:

6. 淋巴瘤生物學(xué): 彌漫性大B細(xì)胞淋巴瘤(DLBCL)的分子機(jī)制研究

7. 表觀遺傳學(xué): CREBBP功能及其對(duì)增強(qiáng)子組蛋白乙酰化(H3K27ac)調(diào)控的研究

8. 藥物篩選: 針對(duì)CREBBP突變淋巴瘤的HDAC3抑制劑等靶向治療研究

9. 基因功能: SECISBP2等基因在淋巴瘤細(xì)胞增殖和凋亡中的作用研究
   培養(yǎng)基: BioVector? MD901 細(xì)胞專(zhuān)用完全培養(yǎng)基（推薦基礎(chǔ)成分：RPMI-1640培養(yǎng)基或IMDM培養(yǎng)基，添加10%胎牛血清和1%青霉素-鏈霉素）
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 維持細(xì)胞密度在2×10? - 1×10? cells/mL之間，每2-3天傳代或更換新鮮培養(yǎng)基
   凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90%胎牛血清 + 10% DMSO）
   質(zhì)控載體: BioVector? pUC19 質(zhì)粒
   宿主菌: BioVector? DH5α 化學(xué)感受態(tài)細(xì)胞
   質(zhì)量控制: 支原體檢測(cè)：陰性；無(wú)菌檢測(cè)：陰性；STR分型：信息缺失，建議使用者自行驗(yàn)證
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存（-150°C至-190°C）
   生物安全等級(jí): 1（需在二級(jí)生物安全柜內(nèi)操作）
   僅供科研使用

English:
BioVector? MD901 Human Diffuse Large B-cell Lymphoma Cell Line Datasheet

Product Name: BioVector? MD901 Cells
Synonyms: BioVector? MD-901
Chinese Name: BioVector? MD901 人彌漫性大B細(xì)胞淋巴瘤細(xì)胞系
English Name: BioVector? MD901 Human Diffuse Large B-cell Lymphoma Cell Line
Product Category: Hematologic Cancer Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Not specified, likely peripheral blood or lymph node
Disease: Diffuse Large B-cell Lymphoma (DLBCL)
Patient Information: Not reported, presumed adult based on cell line establishment period
Morphology: Suspension growth, lymphoblast-like
Growth Characteristics: Suspension
Derivation: Established from a patient sample with Diffuse Large B-cell Lymphoma
Molecular Characteristics:

1. Tumor Subtype: Activated B-cell-like (ABC) subtype

2. EBV Status: Not reported; typically DLBCL cell lines are EBV negative

3. CREBBP Status: CREBBP wild-type; commonly used for CREBBP loss-of-function studies

4. Gene Expression Profile: High bcl-6 mRNA expression (due to Ig gene translocation); miR-28 expression positive; LPP mRNA expression positive

5. Protein Expression: High BCL-6 protein expression level; LPP protein expression positive
   Research Applications:

6. Lymphoma Biology: Molecular mechanism studies of Diffuse Large B-cell Lymphoma (DLBCL)

7. Epigenetics: Studies on CREBBP function and its regulation of enhancer histone acetylation (H3K27ac)

8. Drug Screening: Targeted therapy research including HDAC3 inhibitors for CREBBP-mutant lymphomas

9. Gene Function: Studies on the role of genes such as SECISBP2 in lymphoma cell proliferation and apoptosis
   Culture Medium: BioVector? Complete Medium for MD901 Cells (Recommended composition: RPMI-1640 medium or IMDM medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: Maintain cell density between 2×10? - 1×10? cells/mL; subculture or replenish with fresh medium every 2-3 days
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector? pUC19 Plasmid
   Host Strain: BioVector? DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; STR Profiling: Information missing, recommended user authentication
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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