BioVector? K231 人彌漫大B細(xì)胞淋巴瘤細(xì)胞系 / Human Diffuse Large B-cell Lymphoma Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? K231 細(xì)胞
同義詞: BioVector? KARPAS231; BioVector? KARPAS-231; BioVector? K-231
中文名稱: BioVector? K231 人彌漫性大B細(xì)胞淋巴瘤細(xì)胞系
英文名稱: BioVector? K231 Human Diffuse Large B-cell Lymphoma Cell Line
產(chǎn)品類別: 血液腫瘤細(xì)胞系
物種來源: 人 (Homo sapiens)
組織來源: 外周血
疾病: 彌漫性大B細(xì)胞淋巴瘤 (DLBCL)
患者信息: 71歲女性
形態(tài)特性: 懸浮生長，淋巴母細(xì)胞樣
生長特性: 懸浮生長
構(gòu)建方法: 從彌漫性大B細(xì)胞淋巴瘤患者的外周血樣本中建立
特性特征:

1. 染色體易位: 攜帶 t(14;18)(q32;q21) 和 t(3;11)(q27;q23.1) 易位

2. EB病毒狀態(tài): 未報道，通常DLBCL細(xì)胞系多為EBV陰性

3. 基因表達(dá)特征: 與OCI-LY8等其他DLBCL細(xì)胞系相比，K231細(xì)胞系中miR-28表達(dá)水平較高；BCL-6和LPP蛋白表達(dá)水平較低

4. bcl-6基因狀態(tài): 由于未涉及Ig基因的易位，bcl-6 mRNA在該細(xì)胞系中未見表達(dá)

5. 研究應(yīng)用: 彌漫性大B細(xì)胞淋巴瘤(DLBCL)的生物學(xué)特性研究、基因表達(dá)調(diào)控研究、藥物篩選的重要體外模型
   培養(yǎng)基: BioVector? K231 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：RPMI-1640培養(yǎng)基，添加10-20%胎牛血清和1%青霉素-鏈霉素）
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 維持細(xì)胞密度在2×10? - 1×10? cells/mL之間，每2-3天傳代或更換新鮮培養(yǎng)基
   凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90%胎牛血清 + 10% DMSO）
   質(zhì)控載體: BioVector? pUC19 質(zhì)粒
   宿主菌: BioVector? DH5α 化學(xué)感受態(tài)細(xì)胞
   質(zhì)量控制: 支原體檢測：陰性；無菌檢測：陰性；STR分型：已驗證（參考KARPAS231）
   儲存與運輸: 液氮或干冰運輸與保存（-150°C至-190°C）
   生物安全等級: 1（需在二級生物安全柜內(nèi)操作）
   僅供科研使用

English:
BioVector? K231 Human Diffuse Large B-cell Lymphoma Cell Line Datasheet

Product Name: BioVector? K231 Cells
Synonyms: BioVector? KARPAS231; BioVector? KARPAS-231; BioVector? K-231
Chinese Name: BioVector? K231 人彌漫性大B細(xì)胞淋巴瘤細(xì)胞系
English Name: BioVector? K231 Human Diffuse Large B-cell Lymphoma Cell Line
Product Category: Hematologic Cancer Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Peripheral blood
Disease: Diffuse Large B-cell Lymphoma (DLBCL)
Patient Information: 71-year-old female
Morphology: Suspension growth, lymphoblast-like
Growth Characteristics: Suspension
Derivation: Established from peripheral blood sample of a patient with Diffuse Large B-cell Lymphoma
Characteristics:

1. Chromosomal Translocations: Carries t(14;18)(q32;q21) and t(3;11)(q27;q23.1) translocations

2. EBV Status: Not reported; typically DLBCL cell lines are EBV negative

3. Gene Expression Profile: Compared to other DLBCL cell lines such as OCI-LY8, K231 cells show relatively high miR-28 expression levels and low BCL-6 and LPP protein expression levels

4. bcl-6 Gene Status: Due to the absence of Ig gene involvement in translocations, bcl-6 mRNA expression is not detected in this cell line

5. Research Applications: Important in vitro model for studying DLBCL biology, gene expression regulation, and drug screening
   Culture Medium: BioVector? Complete Medium for K231 Cells (Recommended composition: RPMI-1640 medium supplemented with 10-20% fetal bovine serum and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: Maintain cell density between 2×10? - 1×10? cells/mL; subculture or replenish with fresh medium every 2-3 days
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector? pUC19 Plasmid
   Host Strain: BioVector? DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; STR Profiling: Authenticated (as KARPAS231)
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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