BioVector? pYL-kasOp-FnCas12a2基因編輯質(zhì)粒 / pYL-kasOp-FnCas12a2 Plasmid 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? pYL-kasOp-FnCas12a2 質(zhì)粒
中文名稱: BioVector? pYL-kasOp-FnCas12a2 CRISPR基因編輯質(zhì)粒
英文名稱: BioVector? pYL-kasOp-FnCas12a2 CRISPR Gene Editing Plasmid
產(chǎn)品類別: CRISPR基因編輯質(zhì)粒
質(zhì)粒類型: CRISPR-Cas12a表達載體
用途: 表達鏈霉菌密碼子優(yōu)化的FnCas12a蛋白，用于鏈霉菌基因組修飾 -1
來源信息: 由Yunzi Luo實驗室構(gòu)建并保藏 -1
Addgene編號: 177874 -1
載體骨架: pCRISPomyces-2 -1
插入片段: 鏈霉菌密碼子優(yōu)化的FnCas12a基因 (3909 bp) -1

別名: FnCas12a, FnCpf1 -1
啟動子: kasOp* -1
篩選標(biāo)記:

• 細菌篩選：安普霉素 (Apramycin, 25 μg/mL) -1

• 真核篩選：URA3 -1
  復(fù)制子: 高拷貝 -1
  克隆方法: Gibson Assembly -1
  測序引物:

• 5′ 測序引物: ACCGAACAGGCCATTCACAGA -1

• 3′ 測序引物: TCTGACGCTCAGTGGAACGA -1
  細菌培養(yǎng)條件:

• 推薦菌株: NEB Stable -1

• 培養(yǎng)溫度: 37°C -1

• 抗性: 安普霉素 (25 μg/mL) -1
  相關(guān)質(zhì)粒: BioVector? pYL-kasOp-FnCas12a3 (FnCas12a突變體EP16) -2
  質(zhì)控載體: BioVector? pUC19 質(zhì)粒
  宿主菌: BioVector? DH5α 化學(xué)感受態(tài)細胞
  質(zhì)量控制: 測序驗證：100%正確；無菌檢測：陰性
  儲存與運輸: -20°C或干冰運輸與保存
  僅供科研使用

English:
BioVector? pYL-kasOp-FnCas12a2 Plasmid Datasheet

Product Name: BioVector? pYL-kasOp-FnCas12a2 Plasmid
Chinese Name: BioVector? pYL-kasOp-FnCas12a2 CRISPR基因編輯質(zhì)粒
English Name: BioVector? pYL-kasOp-FnCas12a2 CRISPR Gene Editing Plasmid
Product Category: CRISPR Gene Editing Plasmid
Plasmid Type: CRISPR-Cas12a Expression Vector
Purpose: Expresses Streptomyces codon-optimized FnCas12a for genome modification in Streptomyces -1
Source: Constructed and deposited by the Yunzi Luo Lab -1
Addgene ID: 177874 -1
Vector Backbone: pCRISPomyces-2 -1
Insert: Streptomyces codon-optimized FnCas12a gene (3909 bp) -1
Synonyms: FnCas12a, FnCpf1 -1
Promoter: kasOp* -1
Selection Markers:

• Bacterial Selection: Apramycin (25 μg/mL) -1

• Eukaryotic Selection: URA3 -1
  Replicon: High Copy Number -1
  Cloning Method: Gibson Assembly -1
  Sequencing Primers:

• 5′ Sequencing Primer: ACCGAACAGGCCATTCACAGA -1

• 3′ Sequencing Primer: TCTGACGCTCAGTGGAACGA -1
  Bacterial Growth Conditions:

• Recommended Strain: NEB Stable -1

• Growth Temperature: 37°C -1

• Resistance: Apramycin (25 μg/mL) -1
  Related Plasmid: BioVector? pYL-kasOp-FnCas12a3 (FnCas12a mutant EP16) -2
  Control Vector: BioVector? pUC19 Plasmid
  Host Strain: BioVector? DH5α Chemically Competent Cells
  Quality Control: Sequencing Validation: 100% correct; Sterility: Negative
  Storage and Shipment: Stored and shipped at -20°C or on dry ice
  For Research Use Only

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