BioVector? FBCP-Mix 細(xì)胞系 / FBCP-Mix Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? FBCP-Mix 細(xì)胞
中文名稱: BioVector? FBCP-Mix 混合細(xì)胞系
英文名稱: BioVector? FBCP-Mix Mixed Cell Line
產(chǎn)品類別: 原代混合細(xì)胞培養(yǎng)物 / 組織工程研究模型
物種來源: 牛 (Bos taurus)
組織來源: 角膜 (Cornea)，可能包含角膜基質(zhì)和內(nèi)皮層細(xì)胞
細(xì)胞類型: 混合細(xì)胞群，包含成纖維細(xì)胞樣細(xì)胞和上皮/內(nèi)皮樣細(xì)胞
形態(tài)特性: 混合形態(tài)，可見梭形成纖維細(xì)胞和多邊形上皮樣細(xì)胞
生長特性: 貼壁生長
構(gòu)建方法: 從牛角膜組織分離消化獲得原代細(xì)胞，經(jīng)有限傳代后混合培養(yǎng)，未進(jìn)行克隆純化，保留細(xì)胞異質(zhì)性
特性特征:

1. 異質(zhì)性: 包含多種細(xì)胞類型，模擬角膜組織的細(xì)胞多樣性，適用于組織工程、細(xì)胞間相互作用研究

2. 增殖能力: 混合培養(yǎng)條件下細(xì)胞可維持一定增殖能力，但長期傳代可能導(dǎo)致細(xì)胞比例改變

3. 應(yīng)用: 常用于角膜創(chuàng)傷愈合模型、藥物滲透研究、生物材料相容性測試等
   培養(yǎng)基: BioVector? FBCP-Mix 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：DMEM/F12 (1:1) 培養(yǎng)基，添加10% 胎牛血清，1% 青霉素-鏈霉素，2mM L-谷氨酰胺，1% 非必需氨基酸）
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 當(dāng)細(xì)胞融合度達(dá)到80-90%時(shí)，推薦使用 BioVector? 專用傳代試劑（如0.25%胰蛋白酶-EDTA）消化，按1:2比例進(jìn)行傳代，每2-3天換液一次
   凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90%胎牛血清 + 10% DMSO）
   質(zhì)量控制: 支原體檢測：陰性；無菌檢測：陰性；細(xì)胞活力：復(fù)蘇后活力>80%
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
   生物安全等級(jí): 1
   僅供科研使用

English:
BioVector? FBCP-Mix Cell Line Datasheet

Product Name: BioVector? FBCP-Mix Cells
Chinese Name: BioVector? FBCP-Mix 混合細(xì)胞系
English Name: BioVector? FBCP-Mix Mixed Cell Line
Product Category: Primary Mixed Cell Culture / Tissue Engineering Research Model
Species of Origin: Bovine (Bos taurus)
Tissue of Origin: Cornea, likely including stromal and endothelial layers
Cell Type: Mixed cell population, containing fibroblast-like cells and epithelial/endothelial-like cells
Morphology: Mixed morphology, with spindle-shaped fibroblasts and polygonal epithelial-like cells visible
Growth Characteristics: Adherent
Derivation: Primary cells isolated and digested from bovine corneal tissue, cultured after limited passages without clonal purification, retaining cellular heterogeneity
Characteristics:

1. Heterogeneity: Contains multiple cell types, mimicking the cellular diversity of corneal tissue, suitable for tissue engineering and cell-cell interaction studies

2. Proliferation Capacity: Maintains certain proliferative capacity under mixed culture conditions, but long-term passage may alter cell proportions

3. Applications: Commonly used in corneal wound healing models, drug permeability studies, biocompatibility testing of biomaterials, etc.
   Culture Medium: BioVector? Complete Medium for FBCP-Mix Cells (Recommended composition: DMEM/F12 (1:1) medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, 2mM L-glutamine, 1% non-essential amino acids)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector? Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:2. Medium renewal is recommended every 2-3 days
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Cell Viability: >80% upon recovery
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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