BioVector? Streptomyces sp. X-11085 鏈霉菌菌株 / Streptomyces sp. X-11085 Strain 數(shù)據(jù)說(shuō)明書

產(chǎn)品名稱: BioVector? Streptomyces sp. X-11085 菌種
中文名稱: BioVector? X-11085 鏈霉菌菌株
英文名稱: BioVector? Streptomyces sp. X-11085 Strain
產(chǎn)品類別: 放線菌
菌株類型: 鏈霉菌屬 (Streptomyces)
保藏編號(hào): NRRL 5331 -1-3
分離來(lái)源: 土壤樣品，采集自美國(guó)加利福尼亞州阿林頓 -1-3
主要用途: 生產(chǎn)抗生素L-反式-2-氨基-4-(2-氨基乙氧基)-3-丁烯酸；生產(chǎn)脫落劑L-2-氨基-4-(2-氨基乙氧基)-丁酸；生產(chǎn)5-羥基-L-賴氨酸 -1-2-3

代謝產(chǎn)物:

1. L-反式-2-氨基-4-(2-氨基乙氧基)-3-丁烯酸 (L-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid): 具有抗菌和驅(qū)蟲活性 -1

2. L-2-氨基-4-(2-氨基乙氧基)-丁酸 (L-2-amino-4-(2-aminoethoxy)-butanoic acid, Aviglycine): 可增強(qiáng)果實(shí)乙烯產(chǎn)生，用作脫落劑 -2-3-4

3. 5-羥基-L-賴氨酸 (5-Hydroxy-L-lysine): 羥基化氨基酸 -5
   形態(tài)特征:

4. 氣生菌絲: 豐富，粉末狀，孢子絲呈直或彎曲狀，無(wú)螺旋 -1

5. 孢子: 長(zhǎng)圓柱形，表面光滑至略皺，有鞘膜，大小0.6×0.4至1.0×0.5微米 -1

6. 孢子顏色: 大量孢子呈灰白色、象牙色至淡黃色 -1

7. 基內(nèi)菌絲: 黃色至橙黃色至淺棕色 -1
   培養(yǎng)特征:

• 最適生長(zhǎng)溫度: 28°C -1-3

• 生長(zhǎng)溫度范圍: 28-35°C生長(zhǎng)良好，45°C以上不生長(zhǎng) -1

• 最適pH: 未明確報(bào)道，通常中性偏堿

• 需氧類型: 好氧 -1-3
  生理生化特性:

• 黑色素產(chǎn)生: 在蛋白胨-鐵培養(yǎng)基上高度產(chǎn)黑色素 -1

• 明膠液化: 陽(yáng)性 -1

• 淀粉水解: 陽(yáng)性 -1

• 牛奶: 生長(zhǎng)良好，中等胨化 -1
  培養(yǎng)基: BioVector? ISP-2 培養(yǎng)基（酵母提取物-麥芽提取物瓊脂）；BioVector? 胰蛋白酶大豆肉湯（種子培養(yǎng)基）-1-3
  培養(yǎng)基配方:

• ISP-2: 酵母提取物 4.0g，麥芽提取物 10.0g，葡萄糖 4.0g，瓊脂 20.0g，蒸餾水 1.0L，pH 7.3

• 種子培養(yǎng)基: 胰蛋白酶大豆肉湯（含胰酶消化酪蛋白 15g/L，大豆蛋白胨 5g/L，葡萄糖 2.5g/L，磷酸氫二鉀 2.5g/L，氯化鈉 5g/L）-3

• 發(fā)酵培養(yǎng)基: 葡萄糖 10.0g，蛋白胨 5.0g，酵母提取物 3.0g，F(xiàn)e(NH4)2(SO4)2·6H2O 0.031g，蒸餾水 1.0L -3
  培養(yǎng)溫度: 28°C -1-3
  保存方法: 4°C短期保存（斜面）；-80°C甘油保存或液氮長(zhǎng)期保存
  凍存保護(hù)劑: 20% 甘油 或 10% DMSO
  產(chǎn)品形式: 凍干粉 / 斜面培養(yǎng)物 / 甘油菌
  生物安全等級(jí): 1
  質(zhì)量控制: 無(wú)菌檢測(cè)：陰性；活力檢測(cè)：復(fù)蘇后生長(zhǎng)良好；形態(tài)學(xué)鑒定：符合標(biāo)準(zhǔn)描述；代謝產(chǎn)物檢測(cè)：陽(yáng)性
  儲(chǔ)存與運(yùn)輸: 4-10°C冷藏運(yùn)輸（短期），干冰或液氮運(yùn)輸（長(zhǎng)期）
  僅供科研使用

English:
BioVector? Streptomyces sp. X-11085 Strain Datasheet

Product Name: BioVector? Streptomyces sp. X-11085 Strain
Chinese Name: BioVector? X-11085 鏈霉菌菌株
English Name: BioVector? Streptomyces sp. X-11085 Strain
Product Category: Actinobacteria
Strain Type: Streptomyces species
Deposit Number: NRRL 5331 -1-3
Isolation Source: Soil sample collected from Arlington, California, USA -1-3
Primary Applications: Production of antibiotic L-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid; production of abscission agent L-2-amino-4-(2-aminoethoxy)-butanoic acid; production of 5-hydroxy-L-lysine -1-2-3
Metabolites:

1. L-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid: Exhibits antimicrobial and anthelmintic activity -1

2. L-2-amino-4-(2-aminoethoxy)-butanoic acid (Aviglycine): Enhances ethylene production in fruits, used as an abscission agent -2-3-4

3. 5-Hydroxy-L-lysine: Hydroxylated amino acid -5
   Morphological Characteristics:

4. Aerial Mycelium: Abundant, powdery; sporophores straight to flexuous, no spirals -1

5. Spores: Long cylindrical, smooth to slightly rugose with a sheath, measuring 0.6×0.4 to 1.0×0.5 μm -1

6. Spore Mass Color: Sandy-buff, ivory to light buff -1

7. Substrate Mycelium: Yellow to orangy yellow to light brown -1
   Culture Characteristics:

• Optimal Growth Temperature: 28°C -1-3

• Temperature Range: Grows well at 28-35°C, no growth above 45°C -1

• Optimal pH: Not specifically reported, typically neutral to slightly alkaline

• Oxygen Requirement: Aerobic -1-3
  Physiological and Biochemical Characteristics:

• Melanin Production: Highly chromogenic on peptone-iron medium -1

• Gelatin Liquefaction: Positive -1

• Starch Hydrolysis: Positive -1

• Milk: Good growth, moderate peptonization -1
  Culture Medium: BioVector? ISP-2 Medium (Yeast Extract-Malt Extract Agar); BioVector? Trypticase Soy Broth (seed medium) -1-3
  Medium Composition:

• ISP-2: Yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 20.0g, distilled water 1.0L, pH 7.3

• Seed Medium: Trypticase soy broth (pancreatic digest of casein 15g/L, enzymatic digest of soybean protein 5g/L, glucose 2.5g/L, dipotassium phosphate 2.5g/L, sodium chloride 5g/L) -3

• Fermentation Medium: Glucose 10.0g, peptone 5.0g, yeast extract 3.0g, Fe(NH4)2(SO4)2·6H2O 0.031g, distilled water 1.0L -3
  Culture Temperature: 28°C -1-3
  Storage Methods: Short-term storage at 4°C (slants); long-term storage at -80°C in glycerol or in liquid nitrogen
  Cryoprotectant: 20% glycerol or 10% DMSO
  Product Format: Freeze-dried powder / Slant culture / Glycerol stock
  Biosafety Level: 1
  Quality Control: Sterility: Negative; Viability: Good growth upon revival; Morphological Identification: Conforms to standard description; Metabolite Production: Positive
  Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
  For Research Use Only

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