BioVector? hEM15A 人子宮內(nèi)膜異位癥患者在位內(nèi)膜間質(zhì)細(xì)胞永生化細(xì)胞系 / Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? hEM15A 細(xì)胞
中文名稱: BioVector? hEM15A 人子宮內(nèi)膜異位癥患者在位內(nèi)膜間質(zhì)細(xì)胞永生化細(xì)胞系
英文名稱: BioVector? hEM15A Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line
產(chǎn)品類別: 婦科疾病研究細(xì)胞系
物種來源: 人 (Homo sapiens)
組織來源: 子宮內(nèi)膜（在位內(nèi)膜）
疾病: 子宮內(nèi)膜異位癥
患者信息: 33歲女性
形態(tài)特性: 梭形，成纖維細(xì)胞樣
生長特性: 貼壁生長
構(gòu)建方法: 采用PCD2SV40T質(zhì)粒轉(zhuǎn)染人子宮內(nèi)膜異位癥患者在位內(nèi)膜細(xì)胞，經(jīng)G418篩選和有限稀釋法克隆建立
特性特征:

1. 首次建立: 首個(gè)源自亞洲人子宮內(nèi)膜異位癥組織的永生化在位內(nèi)膜間質(zhì)細(xì)胞系

2. 核型: 正常二倍體核型，染色體結(jié)構(gòu)和數(shù)量未見異常

3. 致瘤性: 裸鼠體內(nèi)接種未觀察到致瘤性

4. 標(biāo)志物表達(dá): 具有子宮內(nèi)膜間質(zhì)細(xì)胞特征；雌激素受體陽性，孕激素受體陽性

5. 傳代能力: 可連續(xù)傳代培養(yǎng)
   培養(yǎng)基: BioVector? hEM15A 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：DMEM/F12 培養(yǎng)基，添加10-15% 胎牛血清和1% 青霉素-鏈霉素）
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
   傳代方法: 當(dāng)細(xì)胞融合度達(dá)到80-90%時(shí)，推薦使用 BioVector? 專用傳代試劑（如0.25%胰蛋白酶-EDTA）消化1-3分鐘，按1:2至1:3比例進(jìn)行傳代，每2-3天換液一次
   凍存液: BioVector? 細(xì)胞凍存液
   質(zhì)量控制: 支原體檢測(cè)：陰性；無菌檢測(cè)：陰性；STR分型：已驗(yàn)證
   STR分型數(shù)據(jù): Amelogenin: X；CSF1PO: 10,11；D12S391: 21,22；D13S317: 8；D18S51: 15,17；D19S433: 13；D21S11: 29,32.2；D2S1338: 19,25；D3S1358: 16,17；D5S818: 10,11；D6S1043: 14,18；D7S820: 11,12；D8S1179: 10,13；FGA: 22,23；Penta E: 14,16；D16S539: 10,12；TH01: 9；TPOX: 8,11；vWA: 14,18
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
   生物安全等級(jí): 1
   僅供科研使用

English:
BioVector? hEM15A Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line Datasheet

Product Name: BioVector? hEM15A Cells
Chinese Name: BioVector? hEM15A 人子宮內(nèi)膜異位癥患者在位內(nèi)膜間質(zhì)細(xì)胞永生化細(xì)胞系
English Name: BioVector? hEM15A Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line
Product Category: Gynecological Disease Research Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Endometrium (eutopic endometrium)
Disease: Endometriosis
Patient Information: 33-year-old female
Morphology: Spindle-shaped, fibroblast-like
Growth Characteristics: Adherent
Derivation: Established by transfecting cells from eutopic endometrium of an endometriosis patient with the PCD2SV40T plasmid, followed by G418 selection and limited dilution cloning
Characteristics:

1. First of its Kind: The first immortalized eutopic endometrium stromal cell line derived from Asian endometriosis tissue

2. Karyotype: Normal diploid karyotype, with no abnormalities in chromosome structure or number observed

3. Tumorigenicity: No tumorigenicity observed upon implantation in nude mice

4. Marker Expression: Exhibits characteristics of endometrium stromal cells; Estrogen Receptor positive, Progesterone Receptor positive

5. Passaging Capacity: Can be passaged continuously
   Culture Medium: BioVector? Complete Medium for hEM15A Cells (Recommended composition: DMEM/F12 medium supplemented with 10-15% fetal bovine serum and 1% Penicillin-Streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector? Subculture Reagent (e.g., 0.25% Trypsin-EDTA) for 1-3 minutes. Subculture at a split ratio of 1:2 to 1:3. Medium renewal is recommended every 2-3 days
   Freeze Medium: BioVector? Cell Freeze Medium
   Quality Control: Mycoplasma: Negative; Sterility: Negative; STR Profiling: Authenticated
   STR Profile Data: Amelogenin: X; CSF1PO: 10,11; D12S391: 21,22; D13S317: 8; D18S51: 15,17; D19S433: 13; D21S11: 29,32.2; D2S1338: 19,25; D3S1358: 16,17; D5S818: 10,11; D6S1043: 14,18; D7S820: 11,12; D8S1179: 10,13; FGA: 22,23; Penta E: 14,16; D16S539: 10,12; TH01: 9; TPOX: 8,11; vWA: 14,18
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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