BioVector? Peronophythora litchii 荔枝霜疫霉菌 / Litchi Downy Blight Fungus 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? Peronophythora litchii 菌種
中文名稱: BioVector? 荔枝霜疫霉菌
英文名稱: BioVector? Peronophythora litchii (Litchi Downy Blight Fungus)
產(chǎn)品類別: 植物病原真菌
菌種類型: 卵菌 (Oomycete)
分類地位: 鞭毛菌亞門，卵菌綱，霜霉目，霜疫霉科 (Peronophythoraceae) -4
拉丁學(xué)名: Peronophythora litchii Chen ex Ko et al. -1-10
同義詞: 荔枝霜霉病菌、荔枝疫病菌 -1
分離來源: 分離自荔枝果實(shí)/果皮 -3-10
主要用途: 植物病害研究；荔枝霜疫霉病病原菌鑒定；殺菌劑篩選；植物-病原互作研究 -3-10
形態(tài)特征:

1. 菌落形態(tài): 在PDA培養(yǎng)基上生長，菌落白色至奶油色

2. -9

3. 無性階段: 孢囊梗呈樹枝狀不對稱分枝，無色，多隔，小梗末端尖，其上著生一個(gè)孢子囊；孢子囊無色至淡褐色，檸檬形或長橢圓形，頂端具乳頭狀突起 -1-4

4. 有性階段: 產(chǎn)生卵孢子，球形，無色至淡黃色，表面光滑 -1-4
   生物學(xué)特性:

• 生長溫度范圍：11-33℃，最適生長溫度：22-25℃ -4

• 孢子囊形成最適溫度：22-25℃ -4

• 孢子囊萌發(fā)：8-22℃形成游動(dòng)孢子，26-30℃直接萌發(fā)成芽管 -4

• 需氧類型：好氧 -3

• 對濕度要求高，高濕條件下致病性強(qiáng) -4
  寄主范圍: 主要侵染荔枝，亦可侵染番木瓜；人工接種可侵染番茄和絲瓜果實(shí) -4
  培養(yǎng)基: BioVector? PDA 培養(yǎng)基（馬鈴薯葡萄糖瓊脂培養(yǎng)基）-3-8
  培養(yǎng)基配方: 馬鈴薯提取液 1.0L，葡萄糖 20.0g，瓊脂 15.0g，pH自然 -3
  培養(yǎng)溫度: 25-28°C -3-8
  保存方法: 4-10°C冷藏保存（短期）；液氮超低溫冷凍保存（長期）-3-5
  凍存保護(hù)劑: 10%甘油 或 5-15% DMSO -5
  凍存方法: 緩慢降溫（1°C/min至-40°C）后轉(zhuǎn)入液氮保存 -5
  復(fù)蘇方法: 38°C水浴快速解凍 -5
  產(chǎn)品形式: 凍干粉 / 斜面培養(yǎng)物 / 甘油菌 -3-8
  生物安全等級: 1 -10
  質(zhì)量控制: 無菌檢測：陰性；活力檢測：復(fù)蘇后生長良好；形態(tài)學(xué)鑒定：符合標(biāo)準(zhǔn)描述
  儲存與運(yùn)輸: 4-10°C冷藏運(yùn)輸（短期），干冰或液氮運(yùn)輸（長期）
  僅供科研使用

English:
BioVector? Peronophythora litchii (Litchi Downy Blight Fungus) Datasheet

Product Name: BioVector? Peronophythora litchii Strain
Chinese Name: BioVector? 荔枝霜疫霉菌
English Name: BioVector? Peronophythora litchii (Litchi Downy Blight Fungus)
Product Category: Plant Pathogenic Fungus
Strain Type: Oomycete
Taxonomic Classification: Mastigomycotina, Oomycetes, Peronosporales, Peronophythoraceae -4
Scientific Name: Peronophythora litchii Chen ex Ko et al. -1-10
Synonyms: Litchi downy blight fungus -1
Isolation Source: Isolated from litchi fruit/pericarp -3-10
Primary Applications: Plant pathology research; Identification of litchi downy blight pathogen; Fungicide screening; Plant-pathogen interaction studies -3-10
Morphological Characteristics:

1. Colony Morphology: Whitish cream colonies on PDA medium -9

2. Asexual Stage: Sporangiophores are tree-like, asymmetrically branched, colorless, with multiple septa; sporangia are colorless to light brown, lemon-shaped or long ellipsoid, with apical papillae -1-4

3. Sexual Stage: Produces oospores, spherical, colorless to light yellow, smooth-walled -1-4
   Biological Characteristics:

• Temperature Range for Growth: 11-33°C, Optimal Growth Temperature: 22-25°C -4

• Optimal Temperature for Sporangia Formation: 22-25°C -4

• Sporangia Germination: 8-22°C (zoospore formation), 26-30°C (direct germination into germ tubes) -4

• Oxygen Requirement: Aerobic -3

• Requires high humidity for infection -4
  Host Range: Mainly infects litchi; can also infect papaya; under artificial inoculation, can infect tomato and loofah fruits -4
  Culture Medium: BioVector? PDA Medium (Potato Dextrose Agar) -3-8
  Medium Composition: Potato extract 1.0L, glucose 20.0g, agar 15.0g, natural pH -3
  Culture Temperature: 25-28°C -3-8
  Storage Methods: 4-10°C refrigeration (short-term); Liquid nitrogen ultra-low temperature freezing (long-term) -3-5
  Cryoprotectant: 10% glycerol or 5-15% DMSO -5
  Freezing Method: Slow cooling (1°C/min to -40°C) followed by transfer to liquid nitrogen -5
  Thawing Method: Rapid thawing in 38°C water bath -5
  Product Format: Freeze-dried powder / Slant culture / Glycerol stock -3-8
  Biosafety Level: 1 -10
  Quality Control: Sterility Test: Negative; Viability Test: Good growth upon revival; Morphological Identification: Conforms to standard description
  Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
  For Research Use Only

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