BioVector? HEI-OC1 小鼠耳蝸毛細(xì)胞系 / Mouse Cochlear Hair Cell Line 數(shù)據(jù)說(shuō)明書(shū)

產(chǎn)品名稱(chēng): BioVector? HEI-OC1 細(xì)胞
同義詞: BioVector? HEIOC1; BioVector? 小鼠耳蝸毛細(xì)胞系
中文名稱(chēng): BioVector? HEI-OC1 小鼠耳蝸毛細(xì)胞系
英文名稱(chēng): BioVector? HEI-OC1 Mouse Cochlear Hair Cell Line
產(chǎn)品類(lèi)別: 感覺(jué)神經(jīng)上皮細(xì)胞系
物種來(lái)源: 小鼠 (Mus musculus)
組織來(lái)源: 耳蝸 (Cochlea)，柯蒂氏器 (Organ of Corti)
細(xì)胞類(lèi)型: 耳蝸毛細(xì)胞前體/祖細(xì)胞
形態(tài)特性: 上皮細(xì)胞樣，貼壁生長(zhǎng)，呈典型的"鋪路石"樣排列 -4-7
生長(zhǎng)特性: 貼壁生長(zhǎng)

特性特征:

1. 溫度敏感型: 在33°C含γ-干擾素條件下增殖迅速且去分化；在37°C無(wú)γ-干擾素條件下分化并表達(dá)毛細(xì)胞標(biāo)志物 -4-7。

2. 標(biāo)志物表達(dá): 分化條件下表達(dá)毛細(xì)胞特異性標(biāo)記，如轉(zhuǎn)錄因子Brn3.1 (POU4F3) 和肌球蛋白VIIA (Myosin VIIA) -2-7。

3. 應(yīng)用廣泛: 是國(guó)際公認(rèn)的用于研究聽(tīng)覺(jué)毛細(xì)胞凋亡、自噬、衰老、氧化應(yīng)激、炎癥反應(yīng)以及耳毒性藥物篩選和聽(tīng)力保護(hù)機(jī)制的金標(biāo)準(zhǔn)體外模型 -3-4-8。
   培養(yǎng)基: BioVector? HEI-OC1 細(xì)胞專(zhuān)用完全培養(yǎng)基（基礎(chǔ)成分為高糖DMEM培養(yǎng)基，添加10%胎牛血清和1%青霉素-鏈霉素）-3-5-9
   培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C (用于分化培養(yǎng)/常規(guī)實(shí)驗(yàn)) -3-4-7
   傳代方法: 當(dāng)細(xì)胞融合度達(dá)到80%-90%時(shí)，推薦使用 BioVector? 專(zhuān)用傳代試劑（如0.25%胰蛋白酶-EDTA），按1:2至1:4比例進(jìn)行傳代，每2-3天換液一次 -4-5-9。
   凍存液: BioVector? 細(xì)胞凍存液（推薦配方：90%胎牛血清 + 10% DMSO）-3-9
   質(zhì)控載體: BioVector? pUC19 質(zhì)粒
   宿主菌: BioVector? DH5α 化學(xué)感受態(tài)細(xì)胞
   質(zhì)量控制: 支原體檢測(cè)：陰性；無(wú)菌檢測(cè)：陰性；細(xì)菌檢測(cè)：陰性 -5；STR鑒定：已通過(guò) -9
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
   生物安全等級(jí): 1
   僅供科研使用

English:
BioVector? HEI-OC1 Mouse Cochlear Hair Cell Line Datasheet

Product Name: BioVector? HEI-OC1 Cells
Synonyms: BioVector? HEIOC1; BioVector? Mouse Cochlear Hair Cell Line
Chinese Name: BioVector? HEI-OC1 小鼠耳蝸毛細(xì)胞系
English Name: BioVector? HEI-OC1 Mouse Cochlear Hair Cell Line
Product Category: Sensory Neuroepithelial Cell Line
Species of Origin: Mouse (Mus musculus)
Tissue of Origin: Cochlea, Organ of Corti
Cell Type: Cochlear hair cell progenitors/precursors
Morphology: Epithelial-like, adherent, exhibiting a characteristic "cobblestone" monolayer arrangement -4-7
Growth Characteristics: Adherent
Characteristics:

1. Temperature-Sensitive: Proliferates and dedifferentiates rapidly at 33°C with interferon-gamma; Differentiates and expresses hair cell markers at 37°C without interferon-gamma -4-7.

2. Marker Expression: Expresses hair cell-specific markers upon differentiation, such as the transcription factor Brn3.1 (POU4F3) and Myosin VIIA -2-7.

3. Broad Applications: It is the gold-standard in vitro model internationally recognized for studying auditory hair cell apoptosis, autophagy, senescence, oxidative stress, inflammation, and for ototoxicity drug screening and hearing protection mechanism research -3-4-8.
   Culture Medium: BioVector? Complete Medium for HEI-OC1 Cells (Composition: High-glucose DMEM supplemented with 10% fetal bovine serum and 1% Penicillin-Streptomycin) -3-5-9
   Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C (for differentiation/standard experiments) -3-4-7
   Subculturing Method: When cells reach 80%-90% confluence, recommended to use BioVector? Subculture Reagent (e.g., 0.25% Trypsin-EDTA) at a split ratio of 1:2 to 1:4. Medium renewal is recommended every 2-3 days -4-5-9.
   Freeze Medium: BioVector? Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO) -3-9
   Control Vector: BioVector? pUC19 Plasmid
   Host Strain: BioVector? DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Bacterial: Negative -5; STR Profiling: Authenticated -9
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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