BioVector? UB/OC-1 小鼠耳蝸毛細(xì)胞系 / Mouse Cochlear Hair Cell Line 數(shù)據(jù)說(shuō)明書(shū)

產(chǎn)品名稱: BioVector? UB/OC-1 細(xì)胞
同義詞: BioVector? University of Bristol/Organ of Corti-1; BioVector? 耳蝸毛細(xì)胞系1號(hào)
中文名稱: BioVector? UB/OC-1 小鼠耳蝸毛細(xì)胞系
英文名稱: BioVector? UB/OC-1 Mouse Cochlear Hair Cell Line
產(chǎn)品類(lèi)別: 感覺(jué)神經(jīng)上皮細(xì)胞系
物種來(lái)源: 小鼠 (Mus musculus)，C57BL/6 背景 -9
組織來(lái)源: 胚胎第13天 (E13) 小鼠耳蝸，柯蒂氏器 (Organ of Corti) -9
細(xì)胞類(lèi)型: 耳蝸毛細(xì)胞前體
構(gòu)建方法: 從攜帶條件性永生化基因 (H-2Kb-tsA58) 的 Immortomouse? 中建立 -9
形態(tài)特性: 上皮細(xì)胞樣
生長(zhǎng)特性: 貼壁生長(zhǎng)
特性特征:

1. 條件永生化: 在33°C含γ-干擾素的條件下增殖 (永生化表型)；在39°C無(wú)γ-干擾素的條件下分化 (非永生化表型) -9。

2. 標(biāo)志物表達(dá): 分化后表達(dá)毛細(xì)胞特征性基因，包括轉(zhuǎn)錄因子 Brn3.1 (POU4F3)、α9 乙酰膽堿受體亞基，以及 fimbrin、myosin VI 和 VIIA -1-3-6。

3. 應(yīng)用: 被廣泛用于研究毛細(xì)胞分化、基因調(diào)控、電生理特性以及探索聽(tīng)覺(jué)毛細(xì)胞再生的治療方法 -1-3-6。
   培養(yǎng)基: BioVector? UB/OC-1 細(xì)胞專用完全培養(yǎng)基（推薦基礎(chǔ)成分：含 Earle's 鹽的 MEM，添加10%胎牛血清、50 U/mL γ-干擾素和 L-谷氨酰胺）-9
   培養(yǎng)條件:永生化/增殖條件： 氣相：95%空氣，5%二氧化碳；溫度：33°C。分化條件： 溫度：39°C (不含γ-干擾素) -9。
   傳代方法: 推薦使用 BioVector? 專用傳代試劑按比例進(jìn)行傳代。
   凍存液: BioVector? 細(xì)胞凍存液
   質(zhì)控載體: BioVector? pUC19 質(zhì)粒
   宿主菌: BioVector? DH5α 化學(xué)感受態(tài)細(xì)胞
   質(zhì)量控制: 支原體檢測(cè)：陰性；無(wú)菌檢測(cè)：陰性
   儲(chǔ)存與運(yùn)輸: 液氮或干冰運(yùn)輸與保存
   生物安全等級(jí): 1
   僅供科研使用

English:
BioVector? UB/OC-1 Mouse Cochlear Hair Cell Line Datasheet

Product Name: BioVector? UB/OC-1 Cells
Synonyms: BioVector? University of Bristol/Organ of Corti-1; BioVector? Organ of Corti cell line number 1 -9
Chinese Name: BioVector? UB/OC-1 小鼠耳蝸毛細(xì)胞系
English Name: BioVector? UB/OC-1 Mouse Cochlear Hair Cell Line
Product Category: Sensory Neuroepithelial Cell Line
Species of Origin: Mouse (Mus musculus), C57BL/6 background -9
Tissue of Origin: Embryonic day 13 (E13) mouse cochlea, Organ of Corti -9
Cell Type: Cochlear hair cell precursors
Derivation: Established from the Immortomouse? harboring a conditional immortalizing gene (H-2Kb-tsA58) -9
Morphology: Epithelial-like
Growth Characteristics: Adherent
Characteristics:

1. Conditionally Immortalized: Proliferates at 33°C with interferon-gamma (immortalized phenotype); Differentiates at 39°C without interferon-gamma (non-immortalized phenotype) -9.

2. Marker Expression: Expresses hair cell-specific genes upon differentiation, including transcription factor Brn3.1 (POU4F3), the α9 acetylcholine receptor subunit, fimbrin, myosin VI, and myosin VIIA -1-3-6.

3. Applications: Widely used to study hair cell differentiation, gene regulation, electrophysiological properties, and to explore therapeutic methods for auditory hair cell regeneration -1-3-6.
   Culture Medium: BioVector? Complete Medium for UB/OC-1 Cells (Recommended composition: MEM with Earle's salts supplemented with 10% FBS, 50 U/mL interferon-gamma, and L-glutamine) -9
   Culture Conditions:Immortalizing/Proliferating Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 33°C. Differentiating Conditions: Temperature: 39°C (without interferon-gamma) -9.
   Subculturing Method: Recommended to use BioVector? Subculture Reagent at an appropriate split ratio.
   Freeze Medium: BioVector? Cell Freeze Medium
   Control Vector: BioVector? pUC19 Plasmid
   Host Strain: BioVector? DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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