BioVector? DH5α λpir大腸桿菌菌株-適用于攜帶R6K復(fù)制起始位點(diǎn)（ori）的質(zhì)粒（如基因敲除載體、自殺性質(zhì)粒）的克隆和擴(kuò)增 / DH5α λpir Strain 數(shù)據(jù)說(shuō)明書(shū)

產(chǎn)品名稱: BioVector? DH5α λpir 感受態(tài)細(xì)胞
中文名稱: BioVector? DH5α λpir 大腸桿菌克隆菌株
英文名稱: BioVector? DH5α λpir E. coli Cloning Strain
產(chǎn)品類別: 感受態(tài)細(xì)胞/克隆菌株
菌株類型: 大腸桿菌 (E. coli)
菌株用途: 適用于攜帶R6K復(fù)制起始位點(diǎn)（ori）的質(zhì)粒（如基因敲除載體、自殺性質(zhì)粒）的克隆和擴(kuò)增
菌株基因型: F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 λ-
特性特征:

1. λpir 溶原菌: 表達(dá)π蛋白（pir基因產(chǎn)物），為攜帶R6K復(fù)制起始位點(diǎn)的質(zhì)粒提供反式復(fù)制必需蛋白，使此類質(zhì)粒能夠在該菌株中穩(wěn)定復(fù)制和維持

2. recA1 和 endA1: 缺失同源重組能力，提高質(zhì)粒插入片段的穩(wěn)定性；缺失非特異性核酸內(nèi)切酶活性，提高質(zhì)粒提取產(chǎn)量和質(zhì)量

3. lacZΔM15: 可與pUC系列載體實(shí)現(xiàn)藍(lán)白斑篩選
   質(zhì)控載體: BioVector? pR6K 質(zhì)粒（含R6K復(fù)制起始位點(diǎn)）
   轉(zhuǎn)化效率: ≥ 1 x 10? cfu/μg pR6K質(zhì)粒DNA
   質(zhì)量控制: 轉(zhuǎn)化效率驗(yàn)證：合格；無(wú)菌檢測(cè)：陰性；藍(lán)白斑篩選功能：陽(yáng)性
   儲(chǔ)存與運(yùn)輸: -80°C或干冰運(yùn)輸與保存
   僅供科研使用

English:
BioVector? DH5α λpir Strain / Competent Cells Datasheet

Product Name: BioVector? DH5α λpir Competent Cells
Chinese Name: BioVector? DH5α λpir 大腸桿菌克隆菌株
English Name: BioVector? DH5α λpir E. coli Cloning Strain
Product Category: Competent Cells / Cloning Strain
Strain Type: E. coli (Escherichia coli)
Strain Application: Suitable for cloning and propagation of plasmids containing the R6K replication origin (ori), such as gene knockout vectors and suicide plasmids
Strain Genotype: F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 λ-
Characteristics:

1. λpir Lysogen: Expresses the π protein (product of the pir gene), which provides the essential replication protein in trans for plasmids carrying the R6K origin of replication, enabling stable replication and maintenance of such plasmids in this strain

2. recA1 and endA1: Lacks homologous recombination activity, improving stability of inserted DNA fragments; lacks non-specific endonuclease activity, improving yield and quality of plasmid DNA preparations

3. lacZΔM15: Allows blue-white screening with pUC series vectors
   Control Vector: BioVector? pR6K Plasmid (containing the R6K replication origin)
   Transformation Efficiency: ≥ 1 x 10? cfu/μg pR6K plasmid DNA
   Quality Control: Transformation Efficiency Validation: Pass; Sterility: Negative; Blue-White Screening Function: Positive
   Storage and Shipment: Stored and shipped at -80°C or on dry ice
   For Research Use Only

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