BioVector? SM10 λpir 大腸桿菌接合/供體菌株 / SM10 λpir Strain 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? SM10 λpir 感受態(tài)細胞
中文名稱: BioVector? SM10 λpir 大腸桿菌接合/供體菌株
英文名稱: BioVector? SM10 λpir E. coli Conjugative/Donor Strain
產(chǎn)品類別: 感受態(tài)細胞/供體菌株
菌株類型: 大腸桿菌 (E. coli)
菌株用途: 適用于作為攜帶R6K復制起始位點（ori）的穿梭質粒或自殺性質粒的接合轉移供體菌株，常用于基因敲除、細菌遺傳學操作和轉座子誘變
菌株基因型: thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Km?) λpir
特性特征:

1. λpir 溶原菌: 表達π蛋白（pir基因產(chǎn)物），為攜帶R6K復制起始位點的質粒提供反式復制必需蛋白，使此類質粒能夠在該菌株中穩(wěn)定維持

2. 接合轉移功能: 含有整合在染色體上的RP4接合轉移系統(tǒng)基因（RP4-2-Tc::Mu），能夠高效地將攜帶oriT（轉移起始位點）的質粒通過接合作用轉移到受體菌株（如假單胞菌、沙門氏菌等其他革蘭氏陰性菌）

3. recA1: 缺失同源重組能力，維持質粒在供體菌中的穩(wěn)定性

4. 鏈霉素敏感: 便于接合后利用鏈霉素進行受體菌篩選（SM10本身對鏈霉素敏感，而許多受體菌具有鏈霉素抗性）

5. 抗性標記: 染色體上含有卡那霉素抗性基因（Km?）
   質控載體: BioVector? pR6K-oriT 質粒（含R6K復制起始位點和接合轉移起始位點oriT）
   接合轉移效率: 經(jīng)測試，可高效轉移至受體菌株
   質量控制: 接合轉移功能驗證：陽性；無菌檢測：陰性；抗性標記驗證：卡那霉素抗性
   儲存與運輸: -80°C或干冰運輸與保存
   僅供科研使用

English:
BioVector? SM10 λpir Strain / Competent Cells Datasheet

Product Name: BioVector? SM10 λpir Competent Cells
Chinese Name: BioVector? SM10 λpir 大腸桿菌接合/供體菌株
English Name: BioVector? SM10 λpir E. coli Conjugative/Donor Strain
Product Category: Competent Cells / Donor Strain
Strain Type: E. coli (Escherichia coli)
Strain Application: Serves as a donor strain for conjugative transfer of shuttle or suicide plasmids containing the R6K replication origin (ori). Commonly used in gene knockout, bacterial genetics, and transposon mutagenesis
Strain Genotype: thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Km?) λpir
Characteristics:

1. λpir Lysogen: Expresses the π protein (product of the pir gene), which provides the essential replication protein in trans for plasmids carrying the R6K origin of replication, enabling stable maintenance of such plasmids in this strain

2. Conjugative Transfer Function: Contains the RP4 conjugation system genes (RP4-2-Tc::Mu) integrated into the chromosome, enabling efficient transfer of plasmids carrying oriT (origin of transfer) to recipient strains (e.g., Pseudomonas, Salmonella, and other Gram-negative bacteria) via conjugation

3. recA1: Lacks homologous recombination activity, maintaining plasmid stability in the donor strain

4. Streptomycin Sensitive: Facilitates counterselection of recipient strains after conjugation using streptomycin (SM10 is streptomycin sensitive, while many recipient strains are streptomycin resistant)

5. Selection Marker: Contains a kanamycin resistance gene (Km?) on the chromosome
   Control Vector: BioVector? pR6K-oriT Plasmid (containing R6K replication origin and oriT transfer origin)
   Conjugation Efficiency: Tested and validated for efficient transfer to recipient strains
   Quality Control: Conjugation Function Validation: Positive; Sterility: Negative; Selection Marker Validation: Kanamycin Resistance
   Storage and Shipment: Stored and shipped at -80°C or on dry ice
   For Research Use Only

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