BioVector? HD-11 雞巨噬細胞系 / Chicken Macrophage Cell Line 數(shù)據(jù)說明書

產(chǎn)品名稱: BioVector? HD-11 細胞
中文名稱: BioVector? HD-11 雞巨噬細胞系
英文名稱: BioVector? HD-11 Chicken Macrophage Cell Line
產(chǎn)品類別: 禽類細胞系
物種來源: 雞 (Gallus gallus)
組織來源: 骨髓
細胞類型: 巨噬細胞
疾病: 白血病
形態(tài)特性: 巨噬細胞樣
生長特性: 貼壁生長
性別: 雌性
構建方法: 由禽白血病病毒MC29株轉化雞骨髓細胞建立
標志物表達: 可被抗雞巨噬細胞單克隆抗體KUL01識別
培養(yǎng)基: BioVector? HD-11 細胞專用完全培養(yǎng)基（基礎成分為RPMI-1640培養(yǎng)基，含4500 mg/L葡萄糖，添加10%胎牛血清）
培養(yǎng)條件: 氣相：95%空氣，5%二氧化碳；溫度：37°C
傳代方法: 推薦使用BioVector? 專用傳代試劑（如Trypsin-EDTA溶液）。吸棄培養(yǎng)基，用不含鈣鎂的PBS潤洗細胞層，加入適量Trypsin-EDTA，37°C孵育至細胞分散（通常5-15分鐘），加入新鮮培養(yǎng)基終止消化并輕輕吹打分散細胞，按比例接種至新培養(yǎng)瓶。建議每2-3天更換一次培養(yǎng)基。
凍存液: BioVector? 細胞凍存液（配方：95% FBS + 5% DMSO）
質控載體: BioVector? pUC19 質粒（用于常規(guī)克隆和測序）
宿主菌: BioVector? DH5α 化學感受態(tài)細胞
質量控制: 支原體檢測：陰性；無菌檢測：陰性
儲存與運輸: 液氮或干冰運輸與保存
生物安全等級: 1
僅供科研使用

BioVector? HD-11 Chicken Macrophage Cell Line Datasheet

Product Name: BioVector? HD-11 Cells
Chinese Name: BioVector? HD-11 雞巨噬細胞系
English Name: BioVector? HD-11 Chicken Macrophage Cell Line
Product Category: Avian Cell Line
Species of Origin: Chicken (Gallus gallus)
Tissue of Origin: Bone Marrow
Cell Type: Macrophage
Disease: Leukemia
Morphology: Macrophage
Growth Characteristics: Adherent
Sex: Female
Derivation: Established by transforming chicken bone marrow cells with avian leukemia virus strain MC29
Marker Expression: Recognized by anti-chicken macrophage monoclonal antibody KUL01
Culture Medium: BioVector? Complete Medium for HD-11 Cells (Composition: RPMI-1640 medium modified with 4500 mg/L glucose, supplemented with 10% fetal bovine serum)
Culture Conditions: Atmosphere: 95% air, 5% CO?; Temperature: 37°C
Subculturing Method: Recommended to use BioVector? Subculture Reagent (e.g., Trypsin-EDTA solution). Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium. Add Trypsin-EDTA solution and incubate at 37°C until cell layer is dispersed (usually within 5 to 15 minutes). Add complete growth medium, aspirate cells by gently pipetting, and distribute appropriate aliquots to new culture vessels. Medium renewal is recommended every 2 to 3 days.
Freeze Medium: BioVector? Cell Freeze Medium (Composition: 95% FBS + 5% DMSO)
Control Vector: BioVector? pUC19 Plasmid (for routine cloning and sequencing)
Host Strain: BioVector? DH5α Chemically Competent Cells
Quality Control: Mycoplasma: Negative; Sterility: Negative
Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
Biosafety Level: 1
For Research Use Only

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