BioVector? CSE-119 細胞系

產(chǎn)品名稱: BioVector? CSE-119 細胞
同義詞: BioVector? Coho Salmon Embryo-119
產(chǎn)品類別: 魚類細胞系
物種來源: 銀大麻哈魚（Coho salmon, Oncorhynchus kisutch）
細胞來源: 胚胎
細胞類型: 成纖維細胞
特性: 自發(fā)永生化細胞系
形態(tài)特性: 成纖維細胞樣
生長特性: 貼壁生長
核型: 二倍體，眾數(shù)為60。另有研究報道為亞四倍體，眾數(shù)為110。
培養(yǎng)基: BioVector? CSE-119 細胞專用完全培養(yǎng)基（基礎成分為EMEM（EBSS）+ 2mM 谷氨酰胺 + 1% 非必需氨基酸 + 10% 胎牛血清）
培養(yǎng)條件: 氣相：5% 二氧化碳，溫度：15-22℃
傳代方法: 當細胞融合達到70-80%時，推薦使用BioVector? 專用傳代試劑（如0.05% 胰蛋白酶/EDTA），按1:3比例進行傳代，傳代頻率約為每10-14天一次。
凍存液: BioVector? 細胞凍存液
應用: 適用于從臨床樣本中分離病毒、病毒增殖（對傳染性胰臟壞死病毒（IPNV）敏感；對病毒性出血性敗血癥病毒（VHSV）和傳染性造血器官壞死病毒（IHNV）不敏感）。
質控載體: BioVector? pUC19 質粒（用于常規(guī)克隆和測序）
宿主菌: BioVector? DH5α 化學感受態(tài)細胞
質量控制: 支原體檢測：陰性 ；無菌檢測：陰性
儲存與運輸: 液氮或干冰運輸與保存
生物安全等級: ACDP 2 級
僅供科研使用

BioVector? CSE-119 Cell Line Datasheet

Product Name: BioVector? CSE-119 Cells
Synonyms: BioVector? Coho Salmon Embryo-119
Product Category: Fish Cell Line
Species of Origin: Coho salmon (Oncorhynchus kisutch)
Tissue of Origin: Whole embryo
Cell Type: Fibroblast
Properties: Spontaneously immortalized cell line
Morphology: Fibroblastic
Growth Characteristics: Adherent
Karyotype: Diploid and modal no. 60. Another study reports a hypotetraploid modal number of 110.
Culture Medium: BioVector? Complete Medium for CSE-119 Cells (Composition: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids + 10% Fetal Bovine Serum)
Culture Conditions: Atmosphere: 5% CO?; Temperature: 15-22°C
Subculturing Method: When cells reach 70-80% confluence, recommended to use BioVector? Subculture Reagent (e.g., 0.05% trypsin/EDTA) at a split ratio of 1:3. Subculture frequency is approximately every 10-14 days.
Freeze Medium: BioVector? Cell Freeze Medium
Applications: Suitable for virus isolation from clinical samples and virus propagation. Susceptible to Infectious Pancreatic Necrosis Virus (IPNV); shows lack of sensitivity to Viral Haemorrhagic Septicaemia Virus (VHSV) and Infectious Haematopoietic Necrosis Virus (IHNV).
Control Vector: BioVector? pUC19 Plasmid (for routine cloning and sequencing)
Host Strain: BioVector? DH5α Chemically Competent Cells
Quality Control: Mycoplasma: Negative ; Sterility: Negative
Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
Biosafety Level: ACDP Hazard Group 2
For Research Use Only

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