Product Name / 產(chǎn)品名稱:
BioVector? pAmpho Plasmid逆轉(zhuǎn)錄病毒包膜表達(dá)載體，專用于生產(chǎn)嗜異性逆轉(zhuǎn)錄病毒
BioVector? pAmpho質(zhì)粒

Description / 描述:
The BioVector? pAmpho plasmid is a retroviral envelope expression vector designed for the production of amphotropic retroviruses. It expresses the 4070A amphotropic envelope protein under the control of the CMV immediate-early promoter . The amphotropic envelope protein enables viral particles to infect a wide range of mammalian cells by interacting with the RAM-1 receptor on the host cell surface . This plasmid is commonly used in conjunction with retroviral packaging cell lines and helper constructs to generate replication-incompetent retroviruses for gene delivery .
BioVector? pAmpho 質(zhì)粒是一種逆轉(zhuǎn)錄病毒包膜表達(dá)載體，專用于生產(chǎn)嗜異性逆轉(zhuǎn)錄病毒。它在CMV立即早期啟動(dòng)子的控制下表達(dá)4070A嗜異性包膜蛋白 。該嗜異性包膜蛋白通過與宿主細(xì)胞表面的RAM-1受體相互作用，使病毒顆粒能夠感染廣泛的哺乳動(dòng)物細(xì)胞 。該質(zhì)粒通常與逆轉(zhuǎn)錄病毒包裝細(xì)胞系和輔助構(gòu)建體聯(lián)合使用，以產(chǎn)生用于基因遞送的無復(fù)制能力的逆轉(zhuǎn)錄病毒 。

Original Source / 原始來源:
Clontech (now Takara Bio) / Clontech公司（現(xiàn)Takara Bio）

Plasmid Type / 質(zhì)粒類型:
Retroviral Envelope Expression Vector / 逆轉(zhuǎn)錄病毒包膜表達(dá)載體

Species / 物種:
Mammalian expression / 哺乳動(dòng)物表達(dá)

Vector Backbone / 載體骨架:
pUC-based / pUC系列骨架

Total Vector Size / 總載體大小:
6732 bp

Key Components / 關(guān)鍵組件:

Gene/Insert Details / 基因/插入片段詳情:

Growth Conditions in Bacteria / 細(xì)菌生長(zhǎng)條件:

Applications / 應(yīng)用:

• Retroviral Packaging / 逆轉(zhuǎn)錄病毒包裝: Used with packaging cell lines (e.g., GP2-293) and transfer vectors to produce amphotropic retroviruses

• Gene Delivery / 基因遞送: Enables infection of a broad range of mammalian cells including human, mouse, rat, and hamster cells

• Viral Pseudotyping / 病毒假型化: Provides amphotropic envelope for pseudotyping retroviral and lentiviral vectors

• Stable Cell Line Generation / 穩(wěn)定細(xì)胞系構(gòu)建: Facilitates generation of stable cell lines via retroviral transduction

• Gene Function Studies / 基因功能研究: Delivers genes of interest to difficult-to-transfect cell types

Related Products / 相關(guān)產(chǎn)品:

Quality Control / 質(zhì)量控制:
Plasmid is verified by restriction digestion and sequencing. Full sequence information is available from the manufacturer.
質(zhì)粒經(jīng)過限制性酶切和測(cè)序驗(yàn)證。完整序列信息可向制造商獲取。

Storage / 存儲(chǔ):

• Plasmid is sent as a bacterial stab in agar or as purified DNA. Upon receipt, streak onto a plate with BioVector? Ampicillin (100 μg/mL), grow a single colony, and prepare a glycerol stock for long-term storage at -80°C.

• 質(zhì)粒以細(xì)菌穿刺菌或純化DNA形式提供。收到后，應(yīng)在含 BioVector? 氨芐青霉素 (100 μg/mL) 的平板上劃線，挑取單克隆，并制備甘油菌于-80°C長(zhǎng)期保存。

Plasmid Reconstitution Protocol / 質(zhì)粒溶解復(fù)配方案:

1. Centrifuge at 5,000×g for 5 min. / 5,000×g離心5分鐘。

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. / 小心打開管蓋，加入20 μl無菌水溶解DNA。

3. Close the tube and incubate for 10 minutes at room temperature. / 蓋緊管蓋，室溫孵育10分鐘。

4. Briefly vortex and spin down. / 短暫渦旋并離心收集。

5. Store the plasmid at -20°C. / 于-20°C保存質(zhì)粒。

6. It is recommended to transform and extract fresh plasmid before use. / 建議使用前進(jìn)行轉(zhuǎn)化并提取新鮮質(zhì)粒。

General Plasmid Transformation Protocol / 通用質(zhì)粒轉(zhuǎn)化方案:

1. Thaw 100 μl competent cells on ice for 10 min, add 2 μl plasmid, incubate on ice for 30 min, heat-shock at 42°C for 60 sec, then incubate on ice for 2 min. / 取100 μl感受態(tài)細(xì)胞冰浴10分鐘，加入2 μl質(zhì)粒，冰浴30分鐘，42°C熱激60秒，再冰浴2分鐘。

2. Add 900 μl antibiotic-free LB medium, incubate at 37°C for 45 min with shaking at 180 rpm. / 加入900 μl無抗生素LB培養(yǎng)基，37°C 180 rpm振蕩培養(yǎng)45分鐘。

3. Centrifuge at 6,000 rpm for 5 min, resuspend pellet in 100 μl supernatant, spread onto LB plate with appropriate antibiotic. / 6,000 rpm離心5分鐘，用100 μl上清重懸沉淀，涂布于含相應(yīng)抗生素的LB平板。

4. Incubate plate inverted at 37°C for 14 hours. / 倒置平板，37°C培養(yǎng)14小時(shí)。

5. Pick single colony into LB medium with antibiotic, incubate at 220 rpm for 14 hours, extract plasmid. / 挑取單克隆至含抗生素的LB培養(yǎng)基，220 rpm培養(yǎng)14小時(shí)，提取質(zhì)粒。

Citation / 引用:
When using this plasmid, please cite the following publications as appropriate:
使用此質(zhì)粒時(shí)，請(qǐng)根據(jù)情況引用以下文獻(xiàn)：

Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. Biotechniques. 1989 Oct;7(9):980-2, 984-6, 989-90.

Important Note / 重要提示:

• The pAmpho plasmid is designed for research use only and should be handled under appropriate biosafety conditions (BSL-1 or BSL-2 depending on the viral constructs used) .

• pAmpho質(zhì)粒僅限研究使用，應(yīng)在適當(dāng)?shù)纳锇踩珬l件下操作（根據(jù)所用病毒構(gòu)建體的不同，為BSL-1或BSL-2級(jí)）。

• For retrovirus production, pAmpho must be co-transfected with a packaging construct (expressing gag-pol) and a transfer vector containing the gene of interest .

• 用于逆轉(zhuǎn)錄病毒生產(chǎn)時(shí)，pAmpho必須與包裝構(gòu)建體（表達(dá)gag-pol）和含有目的基因的轉(zhuǎn)移載體共轉(zhuǎn)染 。

• The amphotropic envelope enables infection of many mammalian cell types, but VSV-G pseudotyping offers an even broader host range .

• 嗜異性包膜能夠感染多種哺乳動(dòng)物細(xì)胞類型，但VSV-G假型化提供更廣泛的宿主范圍 。

BioVector? Ampicillin
BioVector? 氨芐青霉素

For bacterial selection of the pAmpho plasmid and its derivatives.
用于pAmpho質(zhì)粒及其衍生質(zhì)粒的細(xì)菌篩選。

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

電話:400-800-2947

工作QQ/微信同號(hào):1843439339

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