BioVector? CHCC-OU1 Cell Line

English Version

1. Cell Line Overview

• Cell Line Name: BioVector? CHCC-OU1

• Species of Origin:Gallus gallus (Chicken)

• Tissue Origin: Chick embryo cells (CEC)

• Cell Type: Fibroblastic

• Morphology: Transformed; cells pile up and form multilayers

• Parental Line: Derived from chicken helper factor (chf)-negative chick embryo cells

• Year of Establishment: 1984

• Depositor/Reference: H. Ogura et al., Okayama University

2. Description and Applications
BioVector? CHCC-OU1 is a chemically transformed chick embryo fibroblastic cell line established by treatment with the carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) . The cell line was derived from chicken helper factor (chf)-negative chick embryo cells, ensuring the absence of endogenous avian leukosis virus helper activity that could interfere with viral assays .

CHCC-OU1 cells exhibit transformed characteristics including multilayer growth (piling up) and colony formation in soft agar, indicating anchorage-independent growth . Despite these transformed properties in vitro, the cell line is non-tumorigenic as it does not produce tumors either in syngeneic chickens or in nude mice .

The cell line constitutively produces avian endogenous leukosis virus of subgroup E, making it a valuable tool for avian retrovirology research .

3. Key Features

• Chemical Transformation: Established by MNNG treatment of normal chick embryo cells

• Anchorage-Independent Growth: Forms colonies in soft agar

• Non-Tumorigenic: Does not form tumors in syngeneic chickens or nude mice

• Virus Production: Constitutively produces avian endogenous leukosis virus (subgroup E)

• Long-Term Culture: Successfully maintained beyond 400 passages (over 4 years at time of publication)

4. Growth and Culture Conditions

• Recommended Medium: BioVector? DMEM or BioVector? Medium 199, supplemented with 5-10% fetal bovine serum (FBS)

• Growth Characteristics: Transformed fibroblastic cells that pile up and form multilayers; do not exhibit contact inhibition

• Culture Conditions: 41°C, 5% CO? (original culture conditions used 41°C)

• Subculture Ratio: Split when confluent using standard trypsinization procedures

• Adaptation Note: May be adaptable to lower temperatures (37°C) but original characterization was performed at 41°C

5. Quality Control and Safety

• Mycoplasma Testing: Should be tested regularly for mycoplasma contamination

• Biosafety Level: BSL-1 or BSL-2 recommended due to constitutive production of avian leukosis virus

• Virus Shedding: Constitutively produces avian endogenous leukosis virus of subgroup E; appropriate containment should be used

6. Applications

• Avian Retrovirus Research: Source of endogenous avian leukosis virus (subgroup E)

• Viral Interference Assays: Study of viral interference patterns among avian retrovirus subgroups

• Chemical Carcinogenesis Studies: Model for studying chemical transformation of avian cells

• Comparative Oncology: Comparison with non-transformed counterpart (SPCC-OU1) for transformation studies

• Virology: Host cell system for propagation and study of avian viruses

7. Related Cell Lines

• BioVector? SPCC-OU1: The non-transformed counterpart cell line established from the same chf-negative chick embryo cells without MNNG treatment . SPCC-OU1 cells appear normal, exhibit contact inhibition, and do not form colonies in soft agar .

BioVector? CHCC-OU1 細(xì)胞系說(shuō)明書

中文版本

1. 細(xì)胞系概述

• 細(xì)胞系名稱： BioVector? CHCC-OU1

• 物種來(lái)源： 雞 (Gallus gallus)

• 組織來(lái)源： 雞胚細(xì)胞

• 細(xì)胞類型： 成纖維細(xì)胞

• 形態(tài)特征： 轉(zhuǎn)化表型；細(xì)胞堆積形成多層

• 親本系： 源自雞輔助因子（chf）陰性的雞胚細(xì)胞

• 建立年份： 1984年

• 保藏者/參考文獻(xiàn)： H. Ogura 等，岡山大學(xué)

2. 描述與應(yīng)用
BioVector? CHCC-OU1 是通過(guò)致癌劑 N-甲基-N'-硝基-N-亞硝基胍（MNNG） 處理建立的化學(xué)轉(zhuǎn)化雞胚成纖維細(xì)胞系 。該細(xì)胞系源自 雞輔助因子（chf）陰性 的雞胚細(xì)胞，確保了不存在可能干擾病毒檢測(cè)的內(nèi)源性禽白血病病毒輔助活性 。

CHCC-OU1 細(xì)胞表現(xiàn)出轉(zhuǎn)化特性，包括 多層生長(zhǎng)（堆積） 和 在軟瓊脂中形成克隆，表明其具有非錨定依賴性生長(zhǎng) 。盡管在體外具有這些轉(zhuǎn)化特性，該細(xì)胞系是 非致瘤性的，因?yàn)樗谕措u或裸鼠中均不產(chǎn)生腫瘤 。

該細(xì)胞系持續(xù)產(chǎn)生 E亞群內(nèi)源性禽白血病病毒，使其成為禽類逆轉(zhuǎn)錄病毒學(xué)研究的重要工具 。

3. 主要特征

• 化學(xué)轉(zhuǎn)化： 通過(guò)MNNG處理正常雞胚細(xì)胞建立

• 非錨定依賴性生長(zhǎng)： 在軟瓊脂中形成克隆

• 非致瘤性： 在同源雞或裸鼠中不形成腫瘤

• 病毒產(chǎn)生： 持續(xù)產(chǎn)生E亞群內(nèi)源性禽白血病病毒

• 長(zhǎng)期培養(yǎng)： 成功維持超過(guò)400代（發(fā)表時(shí)超過(guò)4年）

4. 生長(zhǎng)與培養(yǎng)條件

• 推薦培養(yǎng)基： BioVector? DMEM 或 BioVector? Medium 199，添加5-10%胎牛血清（FBS）

• 生長(zhǎng)特性： 轉(zhuǎn)化后的成纖維細(xì)胞，堆積形成多層；不表現(xiàn)接觸抑制

• 培養(yǎng)條件： 41°C，5% CO?（原始培養(yǎng)條件使用41°C）

• 傳代比例： 細(xì)胞長(zhǎng)滿后使用標(biāo)準(zhǔn)胰蛋白酶消化程序進(jìn)行傳代

• 適應(yīng)說(shuō)明： 可能可以適應(yīng)較低溫度（37°C），但原始特征鑒定是在41°C下進(jìn)行的

5. 質(zhì)量控制與安全性

• 支原體檢測(cè)： 應(yīng)定期檢測(cè)支原體污染

• 生物安全等級(jí)： 建議使用BSL-1或BSL-2，因?yàn)榧?xì)胞持續(xù)產(chǎn)生禽白血病病毒

• 病毒釋放： 持續(xù)產(chǎn)生E亞群內(nèi)源性禽白血病病毒；應(yīng)使用適當(dāng)?shù)姆雷o(hù)措施

6. 應(yīng)用

• 禽類逆轉(zhuǎn)錄病毒研究： 內(nèi)源性禽白血病病毒（E亞群）的來(lái)源

• 病毒干擾試驗(yàn)： 研究禽類逆轉(zhuǎn)錄病毒亞群間的病毒干擾模式

• 化學(xué)致癌研究： 研究禽類細(xì)胞化學(xué)轉(zhuǎn)化的模型

• 比較腫瘤學(xué)： 與非轉(zhuǎn)化對(duì)照細(xì)胞（SPCC-OU1）進(jìn)行比較，用于轉(zhuǎn)化研究

• 病毒學(xué)： 作為宿主細(xì)胞系統(tǒng)用于禽類病毒的擴(kuò)增和研究

7. 相關(guān)細(xì)胞系

• BioVector? SPCC-OU1： 從相同的chf陰性雞胚細(xì)胞中建立，未經(jīng)MNNG處理的非轉(zhuǎn)化對(duì)照細(xì)胞系 。SPCC-OU1細(xì)胞形態(tài)正常，表現(xiàn)接觸抑制，在軟瓊脂中不形成克隆 。

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