BioVector? Vero-P15 Cell Line

English Version

1. Cell Line Overview

• Cell Line Name: BioVector? Vero-P15

• Species of Origin:Chlorocebus sabaeus (African green monkey)

• Tissue Origin: Kidney

• Cell Type: Epithelial

• Morphology: Adherent (Parental); P15 specifically refers to a characterized adherent passage level

• Parental Line: Derived from the original Vero (Verda Reno) cell line, established from the kidney of a normal adult African green monkey

• ATCC Number: CCL-81 (Parental Vero lineage)

2. Description and Applications
BioVector? Vero-P15 represents a specific, well-characterized passage (Passage 15) of the Vero cell line. Vero cells are one of the most widely used continuous cell lines in virology and vaccine production. Approved by the World Health Organization (WHO) for vaccine manufacturing, Vero cells are highly susceptible to a broad range of viruses. The P15 designation indicates cells that have been carefully maintained to ensure genetic stability and consistent performance in viral susceptibility assays and production workflows.

3. Key Features

• WHO-Approved Substrate: Accepted for use in human and veterinary vaccine production.

• Broad Virus Susceptibility: Supports replication of numerous viruses including poliovirus, respiratory syncytial virus (RSV), yellow fever virus, influenza viruses, and many others.

• Interferon Deficiency: Vero cells are naturally deficient in interferon production, making them exceptionally sensitive to viral infection.

• Genetic Stability: P15 represents a passage level with well-documented stability and characterization.

4. Growth and Culture Conditions

• Recommended Medium: BioVector? High-Glucose DMEM or BioVector? Medium 199, supplemented with 5-10% fetal bovine serum (FBS).

• Growth Characteristics: Adherent cells with a doubling time of approximately 24-28 hours. Requires standard incubation conditions (37°C, 5% CO?).

• Subculture Ratio: Typically split at a ratio of 1:4 to 1:6 when confluent.

• Adaptation Potential: Parental adherent Vero cells (including P15) can be adapted to suspension culture using specific media formulations for large-scale bioreactor applications.

5. Quality Control and Safety

• Sterility Testing: Negative for bacteria, fungi, and mycoplasma.

• Viral Testing: Tested and confirmed negative for adventitious viral agents.

• Tumorigenicity: Vero cells are non-tumorigenic at early passages (including P15) but may become tumorigenic at very high passages; therefore, passage control is critical.

6. Applications

• Isolation and propagation of human and animal viruses

• Production of viral vaccines (poliovirus, rabies, rotavirus, etc.)

• Plaque assays and virus titration

• Virus-host interaction studies

• Cytopathic effect (CPE) assays

• Antiviral drug screening and testing

BioVector? Vero-P15 細(xì)胞系

中文版本

1. 細(xì)胞系概述

• 細(xì)胞系名稱： BioVector? Vero-P15

• 物種來源：Chlorocebus sabaeus (非洲綠猴)

• 組織來源： 腎臟

• 細(xì)胞類型： 上皮細(xì)胞

• 形態(tài)特征： 貼壁生長(zhǎng)（親本型）；P15特指一個(gè)有明確特征描述的貼壁培養(yǎng)代次

• 親本系： 源自原始的Vero（Verda Reno）細(xì)胞系，該細(xì)胞系建立自一只正常成年非洲綠猴的腎臟

• ATCC編號(hào)： CCL-81（親本Vero譜系）

2. 描述與應(yīng)用
BioVector? Vero-P15 代表了一個(gè)特定、充分鑒定的Vero細(xì)胞傳代次（第15代）。Vero細(xì)胞是病毒學(xué)和疫苗生產(chǎn)中使用最廣泛的連續(xù)細(xì)胞系之一。經(jīng)世界衛(wèi)生組織（WHO）批準(zhǔn)用于疫苗生產(chǎn)，Vero細(xì)胞對(duì)多種病毒高度敏感。P15這一名稱表示細(xì)胞經(jīng)過了精心維護(hù)，以確保在病毒敏感性測(cè)定和生產(chǎn)流程中的遺傳穩(wěn)定性和性能一致性。

3. 主要特征

• WHO批準(zhǔn)底物： 被接受用于人用和獸用疫苗生產(chǎn)。

• 廣譜病毒敏感性： 支持多種病毒的復(fù)制，包括脊髓灰質(zhì)炎病毒、呼吸道合胞病毒（RSV）、黃熱病病毒、流感病毒等。

• 干擾素缺陷： Vero細(xì)胞天然存在干擾素產(chǎn)生缺陷，使其對(duì)病毒感染格外敏感。

• 遺傳穩(wěn)定性： P15代表了具有充分文獻(xiàn)記載的穩(wěn)定性和特征描述的代次水平。

4. 生長(zhǎng)與培養(yǎng)條件

• 推薦培養(yǎng)基： BioVector? 高糖DMEM 或 BioVector? Medium 199，添加5-10%胎牛血清（FBS）。

• 生長(zhǎng)特性： 貼壁細(xì)胞，倍增時(shí)間約為24-28小時(shí)。需要標(biāo)準(zhǔn)培養(yǎng)條件（37°C，5% CO?）。

• 傳代比例： 通常在細(xì)胞長(zhǎng)滿后按1:4至1:6的比例進(jìn)行傳代。

• 馴化潛力： 親本貼壁Vero細(xì)胞（包括P15）可以使用特定的培養(yǎng)基配方，馴化為懸浮培養(yǎng)，用于大規(guī)模生物反應(yīng)器應(yīng)用。
  

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5. 質(zhì)量控制與安全性

• 無菌檢測(cè)： 細(xì)菌、真菌和支原體檢測(cè)均為陰性。

• 病毒檢測(cè)： 經(jīng)過檢測(cè)，確證無異源病毒因子。

• 致瘤性： Vero細(xì)胞在早期傳代（包括P15）是非致瘤性的，但在非常高代次時(shí)可能具有致瘤性；因此，嚴(yán)格控制傳代次數(shù)至關(guān)重要。

6. 應(yīng)用

• 人源和動(dòng)物病毒的分離與擴(kuò)增

• 病毒疫苗生產(chǎn)（脊髓灰質(zhì)炎病毒、狂犬病毒、輪狀病毒等）

• 噬斑測(cè)定和病毒滴定

• 病毒與宿主相互作用研究

• 細(xì)胞病變效應(yīng)（CPE）測(cè)定

• 抗病毒藥物篩選和測(cè)試

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