• 物種： 人 (Homo sapiens) 或 嚙齒類 (大鼠/小鼠)

• 細(xì)胞類型： 肝星狀細(xì)胞 (HSCs)，又稱 Ito 細(xì)胞。

• 組織來源： 來源于正常的供體肝臟組織，通過尼科登 (Nycodenz) 密度梯度離心法分離獲得。

• 生長特性： 貼壁生長。

• 主要特征：

  • 靜息狀態(tài) (Quiescent): 在生理條件下，富含維生素 A 脂滴，是體內(nèi)儲存類維生素 A 的主要場所。

  • 激活狀態(tài) (Activated): 受損時會轉(zhuǎn)化為肌成纖維細(xì)胞樣表型，失去脂滴并大量分泌細(xì)胞外基質(zhì)（如 I 型膠原蛋白）。

  • 生物學(xué)標(biāo)記： 靜息態(tài)表達(dá) GFAP, Desmin, 和 LRAT；激活態(tài)表達(dá) alpha-SMA (平滑肌肌動蛋白) 和 Vimentin。

  
  

• 培養(yǎng)指南：

  • 培養(yǎng)基： 推薦使用含 10% 胎牛血清 (FBS) 的 DMEM (高糖) 或?qū)ｉT的星狀細(xì)胞培養(yǎng)基。

  • 基質(zhì)支持： 為了維持靜息狀態(tài)，建議在 Matrigel 等軟基質(zhì)上培養(yǎng)；在塑料或 I 型膠原蛋白表面培養(yǎng)會導(dǎo)致細(xì)胞迅速激活。

  • 培養(yǎng)條件： 37 攝氏度，5% CO2 (二氧化碳)。

  
  

• 主要應(yīng)用：肝纖維化機制研究、維生素 A 代謝、非酒精性脂肪性肝炎 (NASH) 模型構(gòu)建以及抗纖維化藥物篩選。

• Species: Human (Homo sapiens) or Rodent (Rat/Mouse)

• Cell Type: Hepatic Stellate Cells (HSCs), also known as Ito cells.

• Origin: Isolated from donor liver tissue utilizing Nycodenz density gradient centrifugation.

• Growth Properties: Adherent.

• Key Characteristics:

  • Quiescent State: Under physiological conditions, these cells are characterized by the presence of large vitamin A-rich lipid droplets, serving as the body's primary retinoid store.

  • Activated State: Upon liver injury, HSCs transdifferentiate into myofibroblast-like cells, losing their lipid droplets and becoming the principal source of extracellular matrix (ECM) production.

  • Markers: Quiescent cells express GFAP, Desmin, and LRAT. Activated cells are marked by high expression of alpha-SMA and Vimentin.

  
  

• Cell Culture Data:

  • Medium: Recommended DMEM (High Glucose) supplemented with 10% Fetal Bovine Serum (FBS) or specialized HSC media.

  • Substrate: To maintain the quiescent phenotype, culture on soft substrates like Matrigel. Culturing on standard plastic or Collagen Type I induces rapid activation.

  • Incubation: 37 degrees Celsius with 5% CO2.

  
  

• Applications: Modeling hepatic fibrosis and cirrhosis, studying vitamin A metabolism, developing NASH/NAFLD models, and screening anti-fibrotic drug candidates.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號: 1843439339

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