• 物種： 人 (Homo sapiens) 或 嚙齒類 (大鼠/小鼠)

• 細(xì)胞組成： 肝臟中除肝細(xì)胞外的復(fù)雜細(xì)胞群體，主要包括：

  • 肝竇內(nèi)皮細(xì)胞 (LSECs): 構(gòu)成肝血竇壁。

  • 庫(kù)普弗細(xì)胞 (Kupffer Cells): 肝臟駐留巨噬細(xì)胞。

  • 肝星狀細(xì)胞 (HSCs): 儲(chǔ)存維生素 A，參與肝纖維化。

  • 肝內(nèi)膽管上皮細(xì)胞 (Cholangiocytes): 構(gòu)成膽管系統(tǒng)。

  
  

• 主要特征：

  • 免疫與代謝調(diào)節(jié)： NPCs 是肝臟免疫監(jiān)視、炎癥反應(yīng)和細(xì)胞外基質(zhì)沉積的核心。

  • 相互作用： 在生理狀態(tài)下維持肝細(xì)胞功能，在病理狀態(tài)下介導(dǎo)肝損傷。

  
  

• 培養(yǎng)指南：

  • 培養(yǎng)基： 推薦使用專門配制的 NPC 維持培養(yǎng)基（通常含有高質(zhì)量 FBS、胰島素和特定細(xì)胞因子）。

  • 基質(zhì)支持： 通常需要涂覆 I 型膠原蛋白 (Collagen I) 或纖連蛋白。

  • 培養(yǎng)模式： 推薦使用 3D 肝微組織 (Liver Microtissues) 或肝芯片系統(tǒng)，以維持細(xì)胞間的復(fù)雜信號(hào)傳遞。

  
  

• 主要應(yīng)用： 肝毒性評(píng)估、非酒精性脂肪肝 (NAFLD/NASH) 建模、肝纖維化研究及藥物誘導(dǎo)性肝損傷 (DILI) 監(jiān)測(cè)。

• Species: Human (Homo sapiens) or Rodent (Rat/Mouse)

• Cell Composition: A heterogeneous population of cells in the liver excluding hepatocytes, primarily consisting of:

  • Liver Sinusoidal Endothelial Cells (LSECs): Lining the hepatic sinusoids.

  • Kupffer Cells: Resident hepatic macrophages.

  • Hepatic Stellate Cells (HSCs): Vitamin A-storing cells, central to fibrosis.

  • Cholangiocytes: Epithelial cells lining the bile ducts.

  
  

• Key Characteristics:

  • Immune and Metabolic Regulation: NPCs are essential for hepatic immunosurveillance, inflammatory response, and extracellular matrix deposition.

  • Cell-Cell Crosstalk: They maintain hepatocyte functionality under physiological conditions and mediate liver injury during pathogenesis.

  
  

• Cell Culture Data:

  • Medium: Use specialized NPC Maintenance Media (typically supplemented with high-quality FBS, insulin, and specific cytokines).

  • Substrate: Plates often require coating with Collagen Type I or Fibronectin.

  • Culture Formats: Highly recommended for 3D Liver Microtissues or Organ-on-a-Chip systems to preserve physiological cell-cell interactions.

  
  

• Applications: Predicting drug-induced liver injury (DILI), modeling Non-Alcoholic Steatohepatitis (NASH), and studying hepatic fibrosis and inflammation pathways.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號(hào): 1843439339

http://www.nedfriskphoto.com