LLC-PK1 BioVector?豬腎近端小管上皮細胞 (Pig Kidney Proximal Tubule Epithelial Cell Line)

竭誠為您服務！

BioVector NTCC典型培養(yǎng)物保藏中心: 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.; 電話：400-800-2947 工作微信:1843439339 (QQ同號); 郵件：[email protected]; 手機：18901268599; 地址：北京; 已注冊

BioVector? LLC-PK1 豬腎近端小管上皮細胞 (Pig Kidney Proximal Tubule Epithelial Cell Line)

BioVector? LLC-PK1 (豬腎近端小管上皮細胞)

物種：豬 (Sus scrofa)
組織來源：來源于正常的 Hampshire 豬腎臟。
細胞類型：近端小管上皮細胞 (Proximal tubule epithelia)。
生長特性：貼壁生長，在匯合時形成典型的“丘狀” (dome) 結構，表明具有跨上皮液體轉運功能。
主要特征：
- 極性特征：具有明顯的細胞極性，發(fā)育出頂端微絨毛和側基底膜。
- 代謝功能：表現(xiàn)出近端小管特有的酶活性（如亮氨酸氨基肽酶、伽馬谷氨酰轉肽酶）和鈉依賴性葡萄糖轉運。
- 藥物轉運：表達多種外排泵，常被用于研究 P-糖蛋白 (P-gp) 介導的藥物跨膜轉運。
培養(yǎng)基推薦： 90% Medium 199 (M199) + 10% 胎牛血清 (FBS)。
主要應用：腎臟毒理學研究、藥物轉運機制、病毒復制實驗以及蛋白質定向運輸研究。

BioVector? LLC-PK1 (Pig Kidney Proximal Tubule Epithelial Cell Line)

Species: Pig (Sus scrofa)
Origin: Derived from the normal kidney of a Hampshire strain pig.
Cell Type: Proximal tubule epithelia.
Growth Properties: Adherent; forms characteristic "domes" at confluency, indicating transepithelial fluid transport.
Key Characteristics:
- Polarization: Exhibits distinct apical microvilli and basolateral membrane differentiation.
- Metabolic Function: Retains proximal tubule-specific enzymes (e.g., leucine aminopeptidase) and sodium-dependent glucose transport.
- Drug Transport: Widely utilized for P-glycoprotein (P-gp) mediated efflux and drug-drug interaction (DDI) studies.
Culture Medium: 90% Medium 199 (M199) + 10% Fetal Bovine Serum (FBS).
Applications: Renal toxicology, drug transport assays, viral replication studies, and research into protein sorting/trafficking.