PHH BioVector?原代人肝細胞Primary Human Hepatocytes, PHH

價　　格：￥99950
貨　　號：BioVector?-PHH
產(chǎn)　　地：北京

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BioVector NTCC典型培養(yǎng)物保藏中心: 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.; 電話：400-800-2947 工作微信:1843439339 (QQ同號); 郵件：[email protected]; 手機：18901268599; 地址：北京; 已注冊

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BioVector? 原代人肝細胞 (Primary Human Hepatocytes, PHH) 是直接從人體肝臟組織中分離出來的原代細胞。由于其保留了最真實的代謝功能，被視為體外肝毒性和藥物代謝研究的金標(biāo)準(zhǔn)。

PHH 核心技術(shù)參數(shù)表

特性	規(guī)格說明
細胞類型	原代細胞（非永生化，無法傳代）
形態(tài)特征	多邊形/鋪路石狀，具有明顯的細胞核和緊密連接
典型直徑	20 μm 至 30 μm
復(fù)蘇存活率	通常 ≥70%（取決于供體和廠家保證）
功能指標(biāo)	CYP450 酶活性（如 CYP3A4）、白蛋白分泌、尿素合成
推薦載體	鼠尾 I 型膠原蛋白 (Collagen Type I) 涂層培養(yǎng)皿

培養(yǎng)與操作要點

培養(yǎng)基： 必須使用專用的原代肝細胞培養(yǎng)基，通常需添加胰島素、地塞米松及各種生長因子。
鋪板密度：
- 24孔板： 約
  $0.3 minus 0.4 cross 10 to the sixth power$
  cells/well。
- 96孔板： 約
  $0.05 cross 10 to the sixth power$
  cells/well。
存儲環(huán)境： 必須儲存在 液氮蒸汽層（-130°C 以下），嚴(yán)禁存放在 -80°C 冰箱。

主要應(yīng)用領(lǐng)域

藥物代謝 (ADME)： 研究藥物的一相和二相代謝反應(yīng)。
毒理學(xué)評價： 評估藥物誘導(dǎo)的肝損傷 (DILI)。
病毒學(xué)： 研究 乙肝 (HBV) 或 丙肝 (HCV) 的感染機制（PHH 是少數(shù)能自然感染這些病毒的細胞模型）。
3D 模型： 用于肝類器官 (Organoids) 或肝芯片研究。

Primary hepatocytes imaged at 10x

Primary hepatocytes imaged at 20x

Primary Human Hepatocytes (PHH) are not a single "cell line" but are primary cells isolated directly from human liver tissue. They are considered the gold standard for in vitro liver models due to their authentic metabolic activity, though they have a limited lifespan and do not proliferate like immortalised cell lines.

Core Datasheet Specifications

Feature	Specification
Cell Type	Primary human cells (not immortalised)
Morphology	Polygonal/cobblestone shape, distinct nuclei, tight junctions
Typical Diameter	20 μm to 30 μm
Post-thaw Viability	Typically ≥70% (guaranteed by major suppliers)
Yield	Often >5 million viable cells per vial
Functional Markers	CYP3A4, CYP2B6, CYP1A2 activity; Albumin production

Culture & Maintenance

Media Requirements: Requires specialized Hepatocyte Growth Medium supplemented with insulin, hydrocortisone, and growth factors (e.g., HGF).
Seeding Density:
- 6-well plates: 0.9 – 1.1 x 10? cells/mL.
- 96-well plates: 0.9 – 1.0 x 10? cells/mL.
Coating: Vessels must be coated with Collagen Type I or other Extracellular Matrix (ECM) for successful adhesion.
Storage: Must be stored in the vapor phase of liquid nitrogen (-130°C or below).

Key Applications

ADME/Tox: Studying drug metabolism (Phase I/II enzymes) and hepatotoxicity.
Viral Research: Susceptibility to Hepatitis B (HBV) and HCV infections, which many immortalised lines (like HepG2) lack.
3D Models: Validation for spheroid formation and 3D Liver-on-a-chip systems.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號: 1843439339

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