BioVector? Pt.Kich-1 cell line, a rare and specialised model for studying human foetal kidney development and associated paediatric renal pathologies.

• 細胞名稱: Pt.Kich-1

• 物種: 人類 (Homo sapiens)

• 細胞類型: 胎兒腎細胞 (Foetal Kidney)

• 起源背景: 建立自一名 13.5 周齡的人類胎兒腎臟組織。

• 別名: Pt Kich 1, PtKich 1

• 生物安全等級: 1 (建議按照 BSL-2 標準處理人源樣本以確保安全)

• 發(fā)育階段: 代表人類胚胎發(fā)育的關(guān)鍵時期，常用于研究腎臟發(fā)生 (Nephrogenesis)。

• 表型特征: 表現(xiàn)為間充質(zhì) (Mesenchymal) 或未分化的腎前體細胞特征。

• 主要應用:

  • 發(fā)育生物學: 研究人類腎臟在胚胎期的發(fā)育信號通路（如 Wnt 或 Notch 通路）。

  • 病毒學: 常被用作研究某些具有嗜腎性的病毒感染機制的宿主模型。

  • 毒理學: 評估藥物對發(fā)育中腎臟細胞的早期毒性影響。

  
  

• 形態(tài)學: 類成纖維細胞 (Fibroblast-like)，貼壁生長。

• 培養(yǎng)基: DMEM/F12 (1:1) 或 RPMI-1640 + 10% 胎牛血清 (FBS)。

• 培養(yǎng)條件: 37 °C, 5% CO?。

• 傳代比例: 1:2 至 1:4；當細胞達到 80-90% 融合度時使用 Trypsin-EDTA 進行消化傳代。

• 冷凍保存: 90% FBS + 10% DMSO 或使用專門的無血清凍存液。

Cell Line Datasheet - English Version

• Cell Line Name: Pt.Kich-1

• Species: Human (Homo sapiens)

• Cell Type: Foetal Kidney

• Origin: Established from human foetal kidney tissue at approximately 13.5 weeks of gestation.

• Aliases: Pt Kich 1, PtKich 1

• Biosafety Level: 1 (BSL-2 handling is recommended for all human-derived cell lines).

• Developmental Stage: Represents a critical window of human nephrogenesis, providing insights into embryonic kidney maturation.

• Phenotype: Characterised by mesenchymal-like morphology and expression of renal progenitor markers.

• Research Applications:

  • Developmental Biology: Investigating the molecular cues governing the transition from mesenchymal to epithelial cells in the kidney.

  • Virology: Utilised as a substrate for studying the replication and effects of specific renal-tropic viruses.

  • Toxicity Testing: Modelling the effects of pharmaceutical compounds on developing human renal tissues.

  
  

• Morphology: Fibroblast-like; adherent monolayer.

• Culture Medium: DMEM/F12 (1:1 mixture) or RPMI-1640 supplemented with 10% FBS.

• Incubation: 37 °C with 5% CO?.

• Subculture: Split ratio of 1:2 to 1:4 using Trypsin-EDTA; subculture when cells reach 80–90% confluency.

• Storage/Freezing: 90% FBS + 10% DMSO.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號: 1843439339

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