BioVector? UM-SCC-47細(xì)胞系是研究 HPV 陽性頭頸部鱗狀細(xì)胞癌（HNSCC）最廣泛使用的金標(biāo)準(zhǔn)模型。

• 細(xì)胞名稱: UM-SCC-47

• 物種: 人類 (Homo sapiens)

• 疾病類型: 舌鱗狀細(xì)胞癌 (口咽癌)

• 起源背景: 建立自一名患有舌側(cè)鱗狀細(xì)胞癌的男性患者。 [1, 2]

• 生物安全等級(jí): 2 (因含有 HPV 病毒序列，需在 BSL-2 實(shí)驗(yàn)室操作)

• HPV 狀態(tài): HPV-16 陽性。該細(xì)胞系基因組中整合了人乳頭瘤病毒 16 型序列，并持續(xù)表達(dá)致癌蛋白 E6 和 E7。 [1, 2]

• TP53 狀態(tài): 野生型 (Wild-type)。但在 HPV 陽性背景下，p53 蛋白通常被 E6 蛋白降解導(dǎo)致功能失活。 [2]

• 分子特征: 常表現(xiàn)為 p16 蛋白過度表達(dá)（HPV 感染的標(biāo)志）。

• 應(yīng)用領(lǐng)域:

  • 病毒致癌研究: 研究 HPV 如何驅(qū)動(dòng)口咽部腫瘤發(fā)生。

  • 放化療敏感性: HPV 陽性腫瘤通常對(duì)放射線和順鉑更敏感，該細(xì)胞系是研究該現(xiàn)象的核心模型。

  • 免疫治療: 研究針對(duì) HPV 抗原的疫苗或 TCR-T 療法。

  
  

• 形態(tài)學(xué): 類上皮狀，貼壁生長。

• 培養(yǎng)基: DMEM + 10% FBS + 1% 非必需氨基酸 (NEAA) + 1% L-谷氨酰胺。 [1]

• 培養(yǎng)條件: 37 °C, 5% CO?。

• 傳代建議: 使用 Trypsin-EDTA 消化；傳代比例建議為 1:3 至 1:6。

• 冷凍保存: 90% FBS + 10% DMSO。

• Cell Line Name: UM-SCC-47

• Species: Human (Homo sapiens)

• Disease: Squamous cell carcinoma of the tongue (Oropharyngeal Cancer)

• Origin: Established from a male patient with lateral tongue squamous cell carcinoma. [1, 2]

• Biosafety Level: 2 (Requires BSL-2 containment due to the presence of HPV sequences).

• HPV Status:HPV-16 Positive. The genome contains integrated HPV-16 DNA, leading to constitutive expression of the E6 and E7 oncoproteins. [1, 2]

• TP53 Status:Wild-type (WT). However, p53 protein levels are typically suppressed due to E6-mediated degradation. [2]

• Molecular Profile: Characterized by p16 overexpression, a hallmark of HPV-driven malignancies.

• Research Applications:

  • Viral Oncology: Studying the mechanisms of HPV-mediated transformation in head and neck cancers.

  • Treatment Sensitivity: Investigating why HPV+ HNSCC shows superior response to radiotherapy and chemotherapy compared to HPV-negative lines.

  • Targeted Therapy: Used as a platform for testing HPV-specific immunotherapies.

  
  

• Morphology: Epithelial-like; adherent monolayer.

• Culture Medium: DMEM supplemented with 10% FBS, 1% Non-Essential Amino Acids (NEAA), and 1% L-Glutamine. [1]

• Incubation: 37 °C with 5% CO?.

• Subculture: Use Trypsin-EDTA for dissociation; recommended split ratio of 1:3 to 1:6.

• Storage/Freezing: 90% FBS + 10% DMSO.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號(hào): 1843439339

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