BioVector? Streptomyces albus J1074, arguably the most popular "chassis" strain in specialized metabolism research due to its rapid growth and minimized genome.

• 菌株名稱: Streptomyces albus J1074

• 物種: 白色鏈霉菌 (Streptomyces albus)

• 衍生背景: 衍生自 S. albus G (即 DSM 40413)。它是 SalI 限制修飾系統(tǒng)缺陷型變體。

• 生物安全等級(jí): 1 (非致病性環(huán)境細(xì)菌)

• 底盤細(xì)胞 (Chassis): 它是目前異源表達(dá)（Heterologous Expression）次級(jí)代謝產(chǎn)物（如抗生素、抗腫瘤藥物）最常用的宿主之一。

• 基因組特征: 擁有相對(duì)較小的基因組（約 6.8 Mb），且其內(nèi)源性次級(jí)代謝基因簇（BGCs）較少，背景干擾低。

• 生長(zhǎng)優(yōu)勢(shì): 在鏈霉菌屬中生長(zhǎng)極快，在固體培養(yǎng)基上通常 2-3 天即可長(zhǎng)出菌落。

• 遺傳操作: 轉(zhuǎn)化效率高，兼容多種接合（Conjugation）和質(zhì)粒系統(tǒng)。

• 形態(tài)學(xué): 典型的絲狀生長(zhǎng)，產(chǎn)生白色氣生菌絲和灰色孢子。

• 常用培養(yǎng)基:

  • 液體: TSB (Tryptic Soy Broth) 或 YEME。

  • 固體: MS (Mannitol Soya) 瓊脂（用于接合傳代）或 R5 瓊脂。

  
  

• 培養(yǎng)條件: 28-30 °C，液體培養(yǎng)需劇烈震蕩（200-250 rpm）以防止菌絲結(jié)團(tuán)。

• 生理特性: 能夠產(chǎn)生多種內(nèi)源性抗生素（如 Salinomycin），但在工程化研究中通常被用于生產(chǎn)外源產(chǎn)物。

Strain Datasheet - English Version

• Strain Name: BioVector? Streptomyces albus J1074

• Species:Streptomyces albus

• Origin: Derivative of S. albus G (DSM 40413). It is a defective variant of the SalI restriction-modification system.

• Biosafety Level: 1

• Heterologous Host: Widely utilized as a "workhorse" for the heterologous expression of complex natural product biosynthetic gene clusters (BGCs).

• Genome Streamlining: Possesses a streamlined genome (~6.8 Mb) compared to other streptomycetes, reducing metabolic noise and precursor competition.

• Growth Profile: Exceptionally fast growth rate for an actinomycete, typically forming colonies within 48–72 hours on agar.

• Genetic Tractability: Highly amenable to DNA uptake via intergeneric conjugation from E. coli (e.g., S17-1 or ET12567/pUZ8002).

• Morphology: Filamentous mycelial growth; produces white aerial hyphae and grey spores.

• Recommended Media:

  • Liquid: TSB or YEME for biomass and genomic DNA preparation.

  • Solid: MS (Mannitol Soya) for conjugation; R2YE or R5 for regeneration.

  
  

• Incubation:28–30 °C; liquid cultures require vigorous aeration (200–250 rpm) using baffled flasks to ensure proper oxygenation and prevent pelleting.

• Genetics: Its genome sequence is publicly available (GenBank: CP004370.1), facilitating metabolic engineering.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

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