BioVector? MC38-OVA cell line, a cornerstone model in cancer immunotherapy research used to study T-cell responses against a defined "foreign" antigen.

• 細(xì)胞名稱: MC38-OVA (或 MC38-OT-I/OT-II)

• 物種: 小鼠 (Mus musculus)

• 品系背景: C57BL/6

• 細(xì)胞類型: 結(jié)腸癌 (Colon Adenocarcinoma)

• 衍生自: 親本 MC38 細(xì)胞（來源于 C57BL/6 小鼠誘導(dǎo)的結(jié)腸腺癌）。

• 生物安全等級(jí): 1 (若涉及重組病毒轉(zhuǎn)導(dǎo)，建議在 BSL-2 下操作)

• 遺傳修飾: 通過穩(wěn)定轉(zhuǎn)導(dǎo)/轉(zhuǎn)染技術(shù)，使親本 MC38 細(xì)胞持續(xù)表達(dá)卵清蛋白 (Ovalbumin, OVA)。

• 模型用途: OVA 作為一種強(qiáng)效的模型新抗原 (Model Neoantigen)，使其能被表達(dá)相應(yīng) TCR 的 T 細(xì)胞（如 OT-I 對(duì)應(yīng) CD8+ 或 OT-II 對(duì)應(yīng) CD4+）特異性識(shí)別。

• 應(yīng)用領(lǐng)域:

  • 癌癥疫苗研發(fā)與驗(yàn)證。

  • 過繼性 T 細(xì)胞療法 (ACT) 的效力評(píng)估。

  • 免疫檢查點(diǎn)抑制劑 (ICI) 的藥效評(píng)價(jià)。

  
  

• 形態(tài)學(xué): 類上皮細(xì)胞，貼壁生長(zhǎng)。

• 培養(yǎng)基: DMEM 或 RPMI 1640 + 10% FBS + 1% P/S。

• 篩選壓力: 需添加 G418 (Geneticin) 或其他抗生素（取決于載體構(gòu)建）以維持 OVA 的穩(wěn)定表達(dá)。

• 培養(yǎng)條件: 37 °C, 5% CO?。

• 倍增時(shí)間: 約 18-24 小時(shí)。

• 冷凍保存: 90% FBS + 10% DMSO。

Cell Line Datasheet - English Version

• Cell Line Name: MC38-OVA (also known as MC38-OT-I/OT-II)

• Species: Mouse (Mus musculus)

• Strain Background: C57BL/6

• Cell Type: Colon Adenocarcinoma

• Origin: Derivative of the parental MC38 line (a colon adenocarcinoma induced in C57BL/6 mice).

• Biosafety Level: 1

• Modification: Parental MC38 cells stably engineered to constitutively express the Ovalbumin (OVA) protein.

• Mechanism: OVA acts as a potent surrogate tumor-associated antigen. It allows researchers to monitor specific T-cell responses using OT-I (CD8+ specific) or OT-II (CD4+ specific) T-cells.

• Key Applications:

  • Validation of cancer vaccines.

  • Efficacy testing for Adoptive Cell Transfer (ACT).

  • In vivo syngeneic tumor models for Immunotherapy (e.g., anti-PD-1/anti-CTLA-4).

  
  

• Morphology: Epithelial-like, adherent monolayer.

• Culture Medium: DMEM or RPMI 1640 supplemented with 10% FBS and 1% Pen-Strep.

• Selection Pressure: Requires maintenance with G418 (Geneticin) or similar selection markers to ensure stable OVA expression.

• Incubation: 37 °C with 5% CO?.

• Doubling Time: Approximately 18–24 hours.

• Storage/Freezing: 90% FBS + 10% DMSO.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號(hào): 1843439339

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