BioVector?  pDS05自殺型載體 (Suicide Vector) / 等位基因交換載體

• 質(zhì)粒名稱: pDS05

• 質(zhì)粒類型: 自殺型載體 (Suicide Vector) / 等位基因交換載體。

• 用途: 用于細(xì)菌基因組的無痕缺失 (Scarless Deletion)、點(diǎn)突變或基因敲入。

• 衍生來源: 基于 pWM91 或類似的 R6K 復(fù)制子載體改造。 [x]

• 復(fù)制子: R6Kγ ori。該復(fù)制子依賴于 pir 蛋白。在缺乏 pir 基因的宿主中無法復(fù)制，從而迫使質(zhì)粒整合進(jìn)染色體。 [x]

• 反向篩選標(biāo)記: sacB。在含蔗糖的培養(yǎng)基上，該基因產(chǎn)物對細(xì)菌有毒，用于篩選發(fā)生第二次交叉（二橋）并丟失質(zhì)粒骨架的克隆。 [x]

• 抗性標(biāo)記: 氨芐青霉素 (Ampicillin) 或其他（取決于具體變體，最常用為 AmpR）。

• 轉(zhuǎn)移原點(diǎn): oriT。允許通過接合 (Conjugation) 從供體菌（如 S17-1 λpir）轉(zhuǎn)移到受體菌中。 [x]

• 無痕編輯: 實(shí)驗(yàn)結(jié)束后，染色體上不會(huì)留下任何抗性標(biāo)記或疤痕序列。

• 宿主范圍: 廣泛用于革蘭氏陰性菌，特別是 弧菌屬 (Vibrio) 和 腸道桿菌。

Plasmid Datasheet - English Version

• Plasmid Name: pDS05

• Plasmid Type:Suicide Vector / Allelic Exchange Vector.

• Purpose: Used for scarless gene deletion, site-directed mutagenesis, or gene knock-ins in bacterial genomes.

• Origin: Often a derivative of the pWM91 lineage or other R6K-based suicide plasmids. [x]

• Replicon:R6Kγ ori. Requires the pir gene product for replication. It acts as a "suicide" plasmid in standard laboratory strains, forcing integration into the host chromosome. [x]

• Counter-selection Marker:sacB. Encodes levansucrase; confers sensitivity to sucrose. This allows for the selection of the second crossover event where the plasmid backbone is excised. [x]

• Selectable Marker: Usually Ampicillin resistance (AmpR).

• Mobilization:oriT (Origin of Transfer). Enables mobilization via conjugation from helper strains like E. coli S17-1 λpir into the target recipient. [x]

• Scarless Modification: Ensures that no antibiotic resistance markers remain in the genome after the successful completion of the two-step recombination.

• Target Species: Commonly utilized in studies of Vibrio cholerae, Yersinia pestis, and other Enterobacteriaceae.

Supplier生產(chǎn)廠家:

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