• 細胞名稱: ICC3

• DSMZ 編號: ACC 995

• 別名: MGH-ECC4, ECC4 (Cellosaurus ID: CVCL_A1ZB)

• 物種: 人類 (Homo sapiens)

• 性別: 女性，63歲

• 疾病類型: 肝外膽管癌 (Extrahepatic Cholangiocarcinoma, ECC)

• 建立背景: 2016年建立自原發(fā)腫瘤的患者來源異種移植模型 (PDX)。

• 生物安全等級: 1 (在進行基因工程操作時，建議提升至 BSL-2)

• 驅(qū)動突變:

  • KRAS p.G12D: 常見的致癌驅(qū)動突變，通常導(dǎo)致 MAPK 和 PI3K 通路的持續(xù)激活。

  • CTNNB1 p.S33P: 涉及 Wnt/β-catenin 通路的激活。

  
  

• STR 鑒定:

  • Amelogenin: X

  • CSF1PO: 9, 10

  • D13S317: 12, 14

  • D16S539: 11, 12

  • D5S818: 10, 12。

  
  

• 其他特征: 端粒酶活性陰性（TRAP檢測）；多種常見病毒（HIV, HBV, HCV, EBV等）檢測均為陰性。

• 形態(tài)學(xué): 類上皮細胞，貼壁呈單層生長，伴有少量懸浮細胞。

• 倍增時間: 約 30-40 小時。

• 關(guān)鍵操作:

  • 不可丟棄懸浮細胞: 更換培養(yǎng)基時需保留上清液中的細胞，因為它們具有活力。

  • 耗材建議: 細胞在重復(fù)使用的培養(yǎng)瓶中貼壁較差，建議使用一次性高品質(zhì) T75 培養(yǎng)瓶。

  
  

• 培養(yǎng)基: 90% RPMI 1640 + 10% 熱滅活胎牛血清 (h.i. FBS)。

BioVector? ICC3 Cell Line

• Cell Line Name: ICC3

• DSMZ No.:ACC 995

• Aliases: MGH-ECC4, ECC4 (Cellosaurus ID: CVCL_A1ZB)

• Species: Human (Homo sapiens)

• Sex/Age: Female, 63 years old.

• Disease:Extrahepatic Cholangiocarcinoma (ECC).

• Establishment: Established in 2016 from a Patient-Derived Xenograft (PDX) model of the primary tumor.

• Biosafety Level: 1 (Note: Handle at BSL-2 if used in genetic engineering projects).

• Key Mutations:

  • KRAS p.G12D: A potent oncogenic driver affecting cellular proliferation pathways like MAPK/ERK.

  • CTNNB1 p.S33P: A mutation in the β-catenin gene associated with Wnt pathway activation.

  
  

• STR Authentication: Profile includes markers such as D13S317 (12, 14), D7S820 (7, 11), and TH01 (6, 9.3).

• Stability: Negative for telomerase activity via TRAP assay; verified authentic against reference databases.

• Morphology: Epithelial-like, growing as an adherent monolayer with a significant population of viable cells in suspension.

• Doubling Time: Approximately 30-40 hours.

• Yield: Approximately 9 x 10? cells per T75 flask at harvest.

• Technical Tips:

  • Supernatant Management: It is vital not to discard cells in the supernatant during media changes as they are part of the active culture.

  • Adhesion Issues: Cells exhibit poor attachment in re-used flasks; fresh plasticware is recommended for consistent growth.

  
  

• Medium: 90% RPMI 1640 + 10% heat-inactivated FBS.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號: 1843439339

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