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首頁 ? HL-60_CDM BioVector?無血清培養(yǎng)人急性髓系白血病細(xì)胞株Human Peripheral blood; Acute Myeloid Leukemia cell line

HL-60_CDM BioVector?無血清培養(yǎng)人急性髓系白血病細(xì)胞株Human Peripheral blood; Acute Myeloid Leukemia cell line

  • 價(jià)  格:¥0
  • 貨  號(hào):BioVector?-HL-60_CDM
  • 產(chǎn)  地:北京
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BioVector?  HL-60_CDM是經(jīng)典 HL-60細(xì)胞系的**無血清培養(yǎng)(Chemically Defined Medium)**衍生型,廣泛用于研究白血病細(xì)胞的分化、增殖以及在嚴(yán)格控制的化學(xué)環(huán)境下的藥物反應(yīng)。


BioVector? HL-60_CDM 細(xì)胞系數(shù)據(jù)表
參數(shù)詳細(xì)信息
細(xì)胞名稱HL-60_CDM (ACC 11010)
物種來源人類 (Homo sapiens)
組織/疾病外周血;急性髓系白血病 (AML FAB M2)
細(xì)胞形態(tài)類成纖維細(xì)胞/淋巴母細(xì)胞樣;圓形,懸浮生長
生長特性懸浮生長(可在無血清條件下持續(xù)增殖)
倍增時(shí)間40 小時(shí)
培養(yǎng)基配方無血清培養(yǎng)基(如 HL-60 專用 CDM 培養(yǎng)基)。其親本系使用 RPMI 1640 + 10% FBS。
培養(yǎng)環(huán)境37°C,5% CO?,恒溫恒濕培養(yǎng)
傳代方法維持細(xì)胞密度在 0.5–1.5 x 10? 細(xì)胞/ml。每 2-3 天通過稀釋或半換液傳代。
標(biāo)志物CD3-, CD4+, CD13(+), CD15+, CD33+, HLA-DR-, CD34-
生物安全等級(jí)1 級(jí)

HL-60 Cell Line:Acute Myeloid Leukemia Research Essentials

-α-negative myeloblastic leukemia cell line HL-60. (A) HL-60 cells were ...

English Version: HL-60_CDM Cell Line Datasheet

ParameterInformation
NameHL-60_CDM (ACC 11010)
DSMZ No.ACC 11010 (Serum-free derivative of ACC 3)
OrganismHuman (Homo sapiens)
Tissue / DiseasePeripheral blood; Acute Myeloid Leukemia (AML FAB M2)
MorphologyLymphoblast-like; round cells growing in suspension
Growth ModeSuspension
Doubling TimeApproximately 40 hours
Culture MediumChemically Defined Medium (CDM) designed for serum-free growth.
Culture Conditions37°C, 5% CO?, humidified atmosphere
SubculturingMaintain at 0.5–1.5 x 10? cells/ml. Split 1:2 to 1:5 every 2-3 days.
MarkersCD3-, CD4+, CD13(+), CD15+, CD33+, HLA-DR-, CD34-
Biosafety Level1

Key Characteristics / 關(guān)鍵特征
  • Serum-Free Adaptation: HL-60_CDM is specifically adapted for growth in serum-free conditions, making it an ideal model for studying the effects of specific growth factors or cytokines without interference from FBS components.

  • Differentiation Potential: Like its parent line, it can be induced to differentiate into granulocyte-like cells (via DMSO or Retinoic Acid) or macrophage-like cells (via TPA/PMA).

  • Genetic Profile: Carries the amplified MYC gene, a hallmark of the HL-60 lineage.

  • 無血清適應(yīng)性: HL-60_CDM 專門適應(yīng)于無血清環(huán)境,是研究特定生長因子或細(xì)胞因子作用的理想模型,排除了胎牛血清 (FBS) 成分的干擾

  • 分化潛能: 與其親本系一樣,可被誘導(dǎo)向粒細(xì)胞樣細(xì)胞(通過 DMSO 或全反式維甲酸)或類巨噬細(xì)胞(通過 TPA/PMA)分化。

  • 遺傳特征: 攜帶 MYC 基因擴(kuò)增,這是 HL-60 譜系的典型標(biāo)志。

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號(hào): 1843439339

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