P-815 NTCC?小鼠肥大細(xì)胞瘤細(xì)胞株Mouse Mast cell / Mastocytoma

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BioVector ^TM P-815 Cell Line

Parameter	Information
Name	P-815 (also known as P815, Mastocytoma P815)
DSMZ Acc. No.	ACC 1
Organism	Mouse (Mus musculus), Strain DBA/2
Tissue / Disease	Mast cell / Mastocytoma (chemically induced by methylcholanthrene)
Morphology	Lymphocyte-like; round cells growing in suspension (some slight adherence)
Growth Mode	Primarily suspension; DSMZ notes some epithelial-like adherent cells
Doubling Time	Fast; approximately 18–22 hours
Culture Medium	RPMI 1640 supplemented with 10% Fetal Bovine Serum (FBS)
Culture Conditions	37°C, 5% CO?, humidified atmosphere
Subculturing	Maintain at 0.25–1.5 x 10? cells/ml. Split ratio 1:4 to 1:8 every 3-4 days
Applications	Standard target cell for cytotoxic T-cell assays; tumor immunology
Biosafety Level	1

中文版：P-815 細(xì)胞系數(shù)據(jù)表

參數(shù)	詳細(xì)信息
細(xì)胞名稱	P-815 (小鼠肥大細(xì)胞瘤細(xì)胞)
DSMZ 編號(hào)	ACC 1
物種來源	小鼠 (Mus musculus)，品系 DBA/2
組織/疾病	肥大細(xì)胞 / 肥大細(xì)胞瘤 (由甲基膽蒽誘導(dǎo))
細(xì)胞形態(tài)	類淋巴細(xì)胞；圓形懸浮生長 (部分細(xì)胞可能輕微貼壁)
生長特性	主要為懸浮生長；在某些條件下可觀察到少量貼壁細(xì)胞
倍增時(shí)間	快速生長，約 18–22 小時(shí)
培養(yǎng)基配方	RPMI 1640 + 10% 胎牛血清 (FBS)
培養(yǎng)環(huán)境	37°C，5% CO?，恒溫恒濕培養(yǎng)
傳代比例	維持密度在 0.25–1.5 x 10? 細(xì)胞/ml。每 3-4 天按 1:4 至 1:8 比例傳代
主要用途	免疫學(xué)研究；細(xì)胞毒性 T 細(xì)胞 (CTL) 實(shí)驗(yàn)的標(biāo)準(zhǔn)靶細(xì)胞
生物安全等級(jí)	1 級(jí)

Key Characteristics / 關(guān)鍵特征

Target Selection: Highly sensitive to lysis by natural killer (NK) cells and cytotoxic T lymphocytes, making it a gold standard for chromium release assays.
Antigen Expression: Known to express H-2d. It lacks antibody-dependent cell-mediated cytotoxicity (ADCC) effector activity.
靶點(diǎn)選擇：對(duì) NK 細(xì)胞和細(xì)胞毒性 T 淋巴細(xì)胞的裂解高度敏感，是鉻釋放實(shí)驗(yàn) (Chromium release assay) 的金標(biāo)準(zhǔn)靶細(xì)胞。
抗原表達(dá)：表達(dá) H-2d 單倍型。缺乏抗體依賴性細(xì)胞介導(dǎo)的細(xì)胞毒性 (ADCC) 效應(yīng)活性。

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

工作微信/QQ同號(hào): 1843439339