BioVector TM SC4 是一種源自基因改造小鼠的永生化細(xì)胞系，常用于2 型神經(jīng)纖維瘤病 (NF2) 及神經(jīng)系統(tǒng)腫瘤的研究。

• 細(xì)胞名稱： SC4 小鼠神經(jīng)鞘瘤細(xì)胞

• 物種： 小鼠 (Mus musculus)

• 組織來源： 來源于 P0-Cre; Nf2 flox/flox 基因敲除小鼠的神經(jīng)腫瘤

• 細(xì)胞類型： 神經(jīng)嵴來源的雪旺細(xì)胞 (Schwann cells)

• 遺傳特征：Nf2 (merlin) 基因缺陷；該基因的缺失會導(dǎo)致信號通路（如 Hippo 或 Ras/Rac 通路）失調(diào)，進(jìn)而誘發(fā)腫瘤

• 生長狀態(tài)： 貼壁生長 (Adherent)

• 培養(yǎng)基方案：DMEM 高糖培養(yǎng)基 + 10% 胎牛血清 (FBS) + 1% 雙抗 (Penicillin/Streptomycin)

• 培養(yǎng)環(huán)境： 37°C，5% CO?，加濕培養(yǎng)箱

• 傳代比例： 建議 1:3 至 1:6；在細(xì)胞達(dá)到約 80-90% 匯合度時進(jìn)行傳代

• 消化液： 0.25% Trypsin-EDTA

• NF2 疾病模型： 用于研究人 2 型神經(jīng)纖維瘤的發(fā)病機制

• 藥物篩選： 常用于測試針對前庭神經(jīng)鞘瘤的潛在治療藥物（如 Celastrol 等）

• 異種移植： 細(xì)胞可接種于裸鼠或斑馬魚幼體以建立腫瘤異種移植模型

• 凍存液： 90% 完全培養(yǎng)基 + 10% DMSO 或?qū)Ｓ眉?xì)胞凍存液。

BioVector TM

• Origin: Derived from a tumor dissected from a P0-CreB; Nf2 flox/flox (genetically engineered) mouse model.

• Species:Mus musculus (Mouse).

• Cell Type: Glial / Schwann cell.

• Disease: Mouse schwannoma.

• Genetic Status:Nf2 (Neurofibromin 2) conditional knockout.

• Key Use: Studying the molecular mechanisms of schwannoma development, particularly the role of the Hippo pathway and potential therapeutic targets.

• Markers: Cells express Schwann cell markers such as S100β and GFAP.

• Growth Properties: Adherent.

• Medium: Dulbecco's Modified Eagle's Medium (DMEM).

• Supplements: 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.

• Incubation: 37°C in a humidified atmosphere with 5% CO?.

• Subculture: Cells are typically passaged using Trypsin/EDTA solution. When preparing primary cultures, methods often involve mechanical and enzymatic dissociation followed by pre-culturing to maximize purity and adherence.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號: 1843439339

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