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首頁 ? Azotobacter vinelandii BioVector TM維氏固氮菌-維涅蘭德固氮菌-固氮能力和生產(chǎn)海藻酸鹽(Alginate)及聚羥基脂肪酸酯(PHB)

Azotobacter vinelandii BioVector TM維氏固氮菌-維涅蘭德固氮菌-固氮能力和生產(chǎn)海藻酸鹽(Alginate)及聚羥基脂肪酸酯(PHB)

  • 價  格:¥99860
  • 貨  號:BioVector TM -Azotobacter vinelandii
  • 產(chǎn)  地:北京
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BioVector TM Azotobacter vinelandii(維氏固氮菌)是一種大型、革蘭氏陰性、好氧細(xì)菌,因其卓越的固氮能力和生產(chǎn)海藻酸鹽(Alginate)及聚羥基脂肪酸酯(PHB)的潛力而在工業(yè)和科研中備受關(guān)注。

主要菌株技術(shù)參數(shù)
不同菌株根據(jù)其遺傳特性和代謝側(cè)重點有所區(qū)別:
  • DJ 菌株 (NTCC BAA-1303): 最常用的實驗室標(biāo)準(zhǔn)菌株,廣泛用于基因功能研究。它是 OP 菌株的衍生物,轉(zhuǎn)化效率極高,由于產(chǎn)生固氮菌素(Azotobactin),在平板上常呈現(xiàn)典型的綠色

  • O 菌株: 原始“野生型”菌株。其特點是菌落呈黏性或“膠狀”,這源于其極高的海藻酸鹽產(chǎn)量。

  • CA (或 OP) 菌株 (NTCC 13705): O 菌株的“非黏性”變體,通常用于研究固氮酶系統(tǒng)的生化特性。

  • DSM 2289 (NTCC 478):標(biāo)準(zhǔn)模式菌株,主要用于分類學(xué)鑒定和基礎(chǔ)培養(yǎng)實驗。

生長與培養(yǎng)條件
  • 生物安全等級: BSL-1(非致病性,對人體安全)。

  • 最佳生長溫度: 26°C - 30°C。

  • 培養(yǎng)基: 常用 Burk’s 培養(yǎng)基(無氮培養(yǎng)基)。若需大量生長,可添加約 2.5% 的蔗糖或甘露醇作為碳源。

  • 形態(tài)特征: 細(xì)胞呈大卵圓形(2–4 μm),常成對出現(xiàn)。在逆境下可形成胞囊(Cysts),具有較強(qiáng)的抗干燥能力。

  • 氧氣耐受: 專性需氧菌,但具有極高的呼吸速率,以消耗細(xì)胞內(nèi)的氧氣從而保護(hù)其對氧敏感的固氮酶。

Azotobacter vinelandii is a large, Gram-negative, aerobic bacterium primarily known for its ability to fix atmospheric nitrogen and produce valuable biopolymers like alginate and polyhydroxyalkanoates (PHB).

Strain Profiles
Different strains of A. vinelandii are used depending on whether the research focus is on genetics, biofertilizer production, or industrial bioplastics.
  • Strain DJ (NTCC BAA-1303): The standard laboratory strain for genetic studies. It is highly competent for taking up foreign DNA and is a derivative of the nongummy OP strain. It typically turns agar plates green due to the production of the siderophore azotobactin.

  • Strain O: The "wild-type" or original strain, characterized by viscous, gummy colonies due to high alginate production.

  • Strain CA (or OP) (NTCC 13705): A "nongummy" version of strain O that is frequently used for studying nitrogenase systems.

  • DSM 2289 (NTCC 478): A type strain used for taxonomic reference and general cultivation.

Key Characteristics & Growth Requirements
  • Biosafety Level: BSL-1 (Non-pathogenic).

  • Optimum Temperature: 26°C to 30°C.

  • Growth Media:Modified Burk's Medium is the standard for nitrogen-free growth. On solid media, it may require sucrose or mannitol (approx. 2.5%) as a carbon source.

  • Morphology: Large oval cells (2–4 μm), often appearing in pairs or clusters. It can form cysts (resting cells) resistant to desiccation.

  • Oxygen Tolerance: While it is an obligate aerobe, it has high respiration rates to "protect" its oxygen-sensitive nitrogenase enzymes.

Supplier生產(chǎn)廠家:

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: [email protected]

工作微信/QQ同號: 1843439339

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