Azotobacter vinelandii BioVector TM維氏固氮菌-維涅蘭德固氮菌-固氮能力和生產(chǎn)海藻酸鹽(Alginate)及聚羥基脂肪酸酯(PHB)
- 價 格:¥99860
- 貨 號:BioVector TM -Azotobacter vinelandii
- 產(chǎn) 地:北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人:Dr.Xu, Biovector NTCC Inc.
電話:400-800-2947 工作微信:1843439339 (QQ同號)
手機(jī):18901268599
地址:北京
- 已注冊
BioVector TM Azotobacter vinelandii(維氏固氮菌)是一種大型、革蘭氏陰性、好氧細(xì)菌,因其卓越的固氮能力和生產(chǎn)海藻酸鹽(Alginate)及聚羥基脂肪酸酯(PHB)的潛力而在工業(yè)和科研中備受關(guān)注。
主要菌株技術(shù)參數(shù)DJ 菌株 (NTCC BAA-1303): 最常用的實驗室標(biāo)準(zhǔn)菌株,廣泛用于基因功能研究。它是 OP 菌株的衍生物,轉(zhuǎn)化效率極高,由于產(chǎn)生固氮菌素(Azotobactin),在平板上常呈現(xiàn)典型的綠色。
O 菌株: 原始“野生型”菌株。其特點是菌落呈黏性或“膠狀”,這源于其極高的海藻酸鹽產(chǎn)量。
CA (或 OP) 菌株 (NTCC 13705): O 菌株的“非黏性”變體,通常用于研究固氮酶系統(tǒng)的生化特性。
DSM 2289 (NTCC 478):標(biāo)準(zhǔn)模式菌株,主要用于分類學(xué)鑒定和基礎(chǔ)培養(yǎng)實驗。
生物安全等級: BSL-1(非致病性,對人體安全)。
最佳生長溫度: 26°C - 30°C。
培養(yǎng)基: 常用 Burk’s 培養(yǎng)基(無氮培養(yǎng)基)。若需大量生長,可添加約 2.5% 的蔗糖或甘露醇作為碳源。
形態(tài)特征: 細(xì)胞呈大卵圓形(2–4 μm),常成對出現(xiàn)。在逆境下可形成胞囊(Cysts),具有較強(qiáng)的抗干燥能力。
氧氣耐受: 專性需氧菌,但具有極高的呼吸速率,以消耗細(xì)胞內(nèi)的氧氣從而保護(hù)其對氧敏感的固氮酶。

Azotobacter vinelandii is a large, Gram-negative, aerobic bacterium primarily known for its ability to fix atmospheric nitrogen and produce valuable biopolymers like alginate and polyhydroxyalkanoates (PHB).
Strain DJ (NTCC BAA-1303): The standard laboratory strain for genetic studies. It is highly competent for taking up foreign DNA and is a derivative of the nongummy OP strain. It typically turns agar plates green due to the production of the siderophore azotobactin.
Strain O: The "wild-type" or original strain, characterized by viscous, gummy colonies due to high alginate production.
Strain CA (or OP) (NTCC 13705): A "nongummy" version of strain O that is frequently used for studying nitrogenase systems.
DSM 2289 (NTCC 478): A type strain used for taxonomic reference and general cultivation.
Biosafety Level: BSL-1 (Non-pathogenic).
Optimum Temperature: 26°C to 30°C.
Growth Media:Modified Burk's Medium is the standard for nitrogen-free growth. On solid media, it may require sucrose or mannitol (approx. 2.5%) as a carbon source.
Morphology: Large oval cells (2–4 μm), often appearing in pairs or clusters. It can form cysts (resting cells) resistant to desiccation.
Oxygen Tolerance: While it is an obligate aerobe, it has high respiration rates to "protect" its oxygen-sensitive nitrogenase enzymes.
Supplier生產(chǎn)廠家:
BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
BioVector NTCC Inc.
TEL: 400-800-2947
E-mail: [email protected]
工作微信/QQ同號: 1843439339
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