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首頁(yè) ? HCC1739 BL BioVector? cell line愛潑斯坦-巴爾病毒 (EBV) 轉(zhuǎn)化B 淋巴母細(xì)胞系

HCC1739 BL BioVector? cell line愛潑斯坦-巴爾病毒 (EBV) 轉(zhuǎn)化B 淋巴母細(xì)胞系

  • 價(jià)  格:¥49950
  • 貨  號(hào):BioVector?HCC1739 BL
  • 產(chǎn)  地:北京
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BioVector? HCC1739 BL 是一種 B 淋巴母細(xì)胞系。它來(lái)源于一名 51 歲患有原發(fā)性乳腺導(dǎo)管癌的白人女性,是 HCC1739 腫瘤細(xì)胞系的 EBV 轉(zhuǎn)化對(duì)照品(正常對(duì)照)

核心特征
  • 細(xì)胞類型: B 淋巴母細(xì)胞 (B lymphocyte)。

  • 來(lái)源: 人類外周血。

  • 永生化方式: 愛潑斯坦-巴爾病毒 (EBV) 轉(zhuǎn)化。

  • 生長(zhǎng)特性: 懸浮生長(zhǎng);通常形成浮動(dòng)的聚集體或大型細(xì)胞團(tuán)塊。

  • 形態(tài)學(xué): 淋巴母細(xì)胞樣。

培養(yǎng)與操作要求
為保持細(xì)胞的最佳活性,建議遵循以下標(biāo)準(zhǔn):
  • 基礎(chǔ)培養(yǎng)基:RPMI-1640 培養(yǎng)基,需添加 10% 胎牛血清 (FBS)。

  • 培養(yǎng)條件: 37°C,5% CO? 濃度。

  • 接種密度: 維持在

    5×1055 cross 10 to the fifth power
    2×1062 cross 10 to the sixth power
    個(gè)活細(xì)胞/mL 之間。


  • 換液頻率: 每 2 至 3 天補(bǔ)加新鮮培養(yǎng)基或更換培養(yǎng)基,具體取決于細(xì)胞密度。

  • 冷凍保存: 儲(chǔ)存于液氮?dú)庀嘀校皇盏郊?xì)胞后應(yīng)盡快解凍并啟動(dòng)培養(yǎng)。

主要應(yīng)用
  • 遺傳對(duì)照: 作為其配對(duì)腫瘤細(xì)胞系 HCC1739 的生殖系(正常)DNA 對(duì)照,用于基因比對(duì)研究。

  • 免疫學(xué)研究: 用于研究 B 細(xì)胞生物學(xué)、EBV 潛伏感染以及抗原提呈。

  • 癌癥研究: 在乳腺癌模型中,用于基準(zhǔn)化基因突變和藥物反應(yīng)分析。

Bright field observations of HCC1937 cells after seven days of... |  Download Scientific Diagram

FBXL2 targets cyclin D2 for ubiquitination during mitosis. (A) MLE... |  Download Scientific Diagram

基因鑒定 (STR 圖譜)

通過(guò)短串聯(lián)重復(fù)序列 (STR) 分析進(jìn)行身份驗(yàn)證。關(guān)鍵標(biāo)記包括:
  • Amelogenin: X

  • CSF1PO: 11, 12

  • D13S317: 11, 13

  • D16S539: 12, 13

  • TH01: 6, 9.3

  • vWA: 17, 19


The BioVector?
HCC1739 BL is a B lymphoblastoid cell line derived from a 51-year-old Caucasian female with primary ductal carcinoma of the breast. It is the Epstein-Barr virus (EBV) transformed "normal" match to the HCC1739 tumor cell line.
Core Characteristics
  • Cell Type: B lymphoblast (B lymphocyte).

  • Origin: Human peripheral blood.

  • Immortalization: Epstein-Barr virus (EBV) transformation.

  • Growth Properties: Suspension; typically forms floating aggregates or large cell clusters.

  • Morphology: Lymphoblast-like.

Culture & Handling Requirements
To maintain optimal health and viability, use the following specifications:
  • Base Medium:RPMI-1640 Medium supplemented with 10% Fetal Bovine Serum (FBS).

  • Culture Conditions: 37°C in a 5% CO? atmosphere.

  • Seeding Density: Maintain between

    5×1055 cross 10 to the fifth power
    and
    2×1062 cross 10 to the sixth power
    viable cells/mL.


  • Medium Renewal: Add fresh medium or replace every 2 to 3 days, depending on cell density.

  • Cryopreservation: Store in liquid nitrogen vapor phase; cells should be thawed and initiated quickly upon receipt.

Applications
  • Genetic Control: Serves as a germline (normal) DNA control for studies involving its paired tumor line, HCC1739.

  • Immunology Research: Used for studying B-cell biology, EBV latency, and antigen presentation.

  • Cancer Research: Benchmarking genetic mutations and drug responses in breast cancer models.

Genetic Authentication (STR Profile)
Authenticity is verified via Short Tandem Repeat (STR) analysis. Key markers include:
  • Amelogenin: X

  • CSF1PO: 11, 12

  • D13S317: 11, 13

  • D16S539: 12, 13

  • TH01: 6, 9.3

  • vWA: 17, 19

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

E-mail:[email protected]

電話:400-800-2947

工作QQ/微信同號(hào):1843439339

網(wǎng)址 www.nedfriskphoto.com


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