BioVector? AD12345678（通常簡稱為 AD1-8）是一種專門用于研究 **ABC 轉(zhuǎn)運蛋白（ATP結(jié)合盒轉(zhuǎn)運蛋白）和多藥耐藥機制的釀酒酵母（Saccharomyces cerevisiae）**實驗室工程菌株。

• 宿主物種： 釀酒酵母 (Saccharomyces cerevisiae)。

• 基因型（敲除列表）： 該菌株敲除了 8 個主要的轉(zhuǎn)運蛋白基因：

  • yor1Δ

  • snq2Δ

  • pdr5Δ

  • pdr10Δ

  • pdr11Δ

  • ycf1Δ

  • pdr3Δ

  • pdr15Δ

  
  

1. 高度敏感性： 由于缺失了上述基因，AD12345678 對多種異物和藥物（如 羅丹明B、寡霉素、4-NQO）表現(xiàn)出極高的敏感性。

2. 異源表達平臺： 常被用作測試植物或人類 ABC 轉(zhuǎn)運蛋白（如 AaABCG40 或 CjMDR1）底物特異性和轉(zhuǎn)運活性的首選平臺。

3. 生物化學(xué)分析： 廣泛應(yīng)用于 ATPase 活性測定和藥物敏感性實驗，用于表征如 Yor1p 等蛋白的生化特性。

相關(guān)菌株對比

• AD2345678： 與 AD12345678 類似，但保留了 YOR1 基因，常作為研究 Yor1p 功能的對照組。

BioVector?

• Host Organism:Saccharomyces cerevisiae.

• Genotype (Deletions): The strain lacks eight major transporters:

  • yor1Δ

  • snq2Δ

  • pdr5Δ

  • pdr10Δ

  • pdr11Δ

  • ycf1Δ

  • pdr3Δ

  • pdr15Δ.

  
  

• Hypersensitivity: Due to these deletions, AD12345678 is highly sensitive to a wide range of xenobiotics and drugs that would normally be pumped out by the cell, such as rhodamine B, oligomycin, and 4-NQO.

• Expression Platform: It is widely used as a "clean" background to test the substrate specificity and transport activity of plant or human ABC transporters, such as the Artemisia annuaAaABCG40 transporter or the CjMDR1 transporter.

• Assay Utility: It is frequently used in ATPase activity assays and drug sensitivity tests to characterize the biochemical properties of overexpressed proteins like Yor1p.

• AD2345678: A closely related strain that retains the YOR1 gene, often used as a control for Yor1p-specific studies.

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