NTCC? ST9671 是一種經(jīng)基因改造的解脂耶羅維亞酵母（Yarrowia lipolytica）菌株，專門用于高產(chǎn)植物抗氧化劑——白藜蘆醇（resveratrol）。

• 宿主物種：解脂耶羅維亞酵母 (Yarrowia lipolytica)，這是一種非模式產(chǎn)油酵母，在生物工程中被廣泛用作“細(xì)胞工廠”。

• 保存編號(hào)：該菌株已保存在 Euroscarf 菌種庫(kù)，訪問(wèn)編號(hào)為 Y41418。

• 衍生關(guān)系：它是由親本菌株 ST6512 改造而來(lái)的。

• 多拷貝集成：整合了 5 個(gè)拷貝的白藜蘆醇生物合成基因，包括來(lái)自植物和細(xì)菌的 FjTAL、At4CL1 和 VvVST1。

• 解除反饋抑制：過(guò)表達(dá)了莽草酸途徑中的關(guān)鍵基因突變體（YlARO4K221L 和 YlARO7G139S），以消除天然代謝調(diào)節(jié)對(duì)產(chǎn)量的限制。

• 白藜蘆醇產(chǎn)量：在小規(guī)模培養(yǎng)中產(chǎn)量可達(dá) 409-525 mg/L。

• 發(fā)酵罐表現(xiàn)：在受控的補(bǔ)料分罐發(fā)酵（fed-batch）中，其產(chǎn)量高達(dá) 12.4 g/L，這是目前報(bào)道的該類化合物從頭合成（de novo production）的最高水平之一。

BioVector? ST9671 strain is a genetically engineered yeast (Yarrowia lipolytica) developed for the high-level production of the plant antioxidant resveratrol and other aromatic compounds. The strain has been deposited with the Euroscarf collection under accession number Y41418.

• Host Organism: The strain is a non-conventional yeast, Yarrowia lipolytica, derived from the parental strain ST6512.

• Genetic Modifications: It was engineered by integrating five additional copies of the resveratrol biosynthetic genes (FjTAL, At4CL1, and VvVST1) and overexpressing feedback-insensitive alleles of the key genes in the shikimate pathway (YlARO4K221L and YlARO7G139S).

• Function: ST9671 is a top-producing strain engineered to produce high levels of resveratrol, reaching a titer of up to 409.0 mg/L in small-scale cultivation and 12.4 g/L in a controlled fed-batch bioreactor, which was the highest reported titer for de novo resveratrol production at the time of the study.

• Research Context: This strain is primarily used in metabolic engineering research to explore the potential of Yarrowia lipolytica as a host organism for producing commercially viable levels of valuable metabolites for industries like food, medical, and cosmetic applications.