NTCC? JW5553 E.coli strain是一種在生命科學(xué)研究中使用的大腸桿菌（Escherichia coli）變異菌株。

• 菌株來(lái)源：它是BioVector NTCC Inc.典型培養(yǎng)物保藏中心開(kāi)發(fā)，包含了一系列將大腸桿菌（K-12 BW25113 親本株）特定基因逐一敲除后的單基因缺失突變株。

• 基因缺失：JW5553 的特定缺失基因是 argB 基因。

  • argB 基因負(fù)責(zé)編碼乙酰谷氨酸激酶（Acetylglutamate kinase），這是生物體合成精氨酸（Arginine）過(guò)程中的關(guān)鍵酶。

  
  

• 生物學(xué)特性：由于缺失了 argB 基因，JW5553 無(wú)法在缺乏精氨酸的最小培養(yǎng)基（Minimal Medium）中生長(zhǎng)，是一種典型的精氨酸缺陷型菌株。

主要研究用途

1. 代謝途徑研究：作為實(shí)驗(yàn)對(duì)照組（陰性對(duì)照），用于驗(yàn)證其他生物是否具有功能性的精氨酸合成路徑。

2. 基因功能驗(yàn)證：研究人員通過(guò)將其他基因?qū)?JW5553，觀察其是否能恢復(fù)精氨酸合成能力，從而確定該基因的功能。

3. 生物工程：在開(kāi)發(fā)能夠高產(chǎn)特定氨基酸或生物化學(xué)品的工業(yè)菌株時(shí)，JW5553 常被用作基礎(chǔ)底盤細(xì)胞進(jìn)行基因改造。

• Strain Origin: It is derived from the parent strain E. coli K-12 BW25113.

• Genetic Mutation: This specific strain has a deletion of the argB gene.

• Biological Function: The argB gene encodes the enzyme acetylglutamate kinase, which is essential for the biosynthesis of the amino acid arginine.

• Phenotype: Because it lacks argB, JW5553 is an arginine auxotroph. This means it cannot produce its own arginine and requires it to be added to its growth medium to survive.

1. Metabolic Engineering: It is used as a "chassis" or base strain for engineering microbes to produce specific chemicals or amino acids (e.g., N-ethyl-L-glutamine).

2. Gene Complementation Studies: Researchers use JW5553 to test whether a gene from another organism can replace the missing argB function, helping to confirm the function of unknown genes.

3. Fundamental Biology: It serves as a tool for studying the complex regulatory networks of amino acid metabolism in bacteria.

JW5553 is a laboratory-grade bacterium used exclusively for scientific experiments. It is not a probiotic or a health-related strain found in food products. If you are looking for information on a "health strain," you may be looking for a different organism with a similar name, such as a Lactobacillus or Bifidobacterium strain.

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