BioVector? NTCC? KT2440惡臭假單胞菌NTCC? (Pseudomonas putida)

The KT2440 strain is a prominent, non-pathogenic strain of the soil bacterium Pseudomonas putida. It is widely recognized in industrial biotechnology and environmental science as a robust, safe chassis for genetic engineering, metabolic studies, and bioremediation applications. Description and Characteristics Organism: Pseudomonas putida. Strain Designation: KT2440 (also known by collection numbers DSM 6125 or ATCC 47054). Origin: It is a derivative of the parental toluene-degrading bacterium P. putida mt-2, which was originally isolated from soil in 1963. The KT2440 strain was created by removing the large pWW0 TOL plasmid. Primary Use: Functions as a robust model organism and host for recombinant DNA experiments, industrial bioproduction, and environmental bioremediation projects. Morphology: It is a Gram-negative, rod-shaped bacterium. Metabolism: Highly versatile; it possesses the metabolic pathways to utilize a wide range of carbon sources, including many complex aromatic compounds. Oxygen Requirement: It is an obligate aerobe, requiring oxygen for growth. Optimal Temperature: It is a mesophile that grows best at 30°C. Biosafety Level: It has been certified by the NIH as a safe Host-Vector Biosafety Level 1 (HV1) system, suitable for use in standard laboratory P1 or ML1 environments. Research Applications Non-Pathogenic: Its lack of virulence factors (such as the type III secretion system found in related pathogens like P. aeruginosa) was a key reason it was certified as a safe laboratory strain for genetic engineering. Bioremediation: Its ability to tolerate and metabolize a variety of toxic chemicals and complex carbon sources makes it ideal for converting industrial waste into valuable products. Synthetic Biology: A large number of genetic tools (plasmids, transposons) have been developed for its manipulation, making it an effective "chassis" organism for synthetic biology applications. Culture Conditions Culture Medium: Standard Luria–Bertani (LB) broth or agar plates are typically used for general laboratory growth. Minimal media (e.g., M9 minimal salts medium) can be used when researchers need to supplement specific carbon sources like citrate or glucose. Temperature: Cultures should be grown at 30°C for optimal growth. Aeration: Requires adequate aeration and aerobic conditions for growth. KT2440 菌株是惡臭假單胞菌 (Pseudomonas putida) 中一種著名、無致病性的土壤細(xì)菌菌株。它在工業(yè)生物技術(shù)和環(huán)境科學(xué)中被廣泛認(rèn)可，是一種健壯、安全的基因工程、代謝研究和生物修復(fù)應(yīng)用的底盤生物。 描述和特征 生物體： Pseudomonas putida (惡臭假單胞菌)。 菌株名稱： KT2440（也稱為收藏號 DSM 6125 或 ATCC 47054）。 來源： 它是耐甲苯降解的親代惡臭假單胞菌 mt-2 菌株的衍生物，mt-2 于 1963 年從土壤中分離得到。KT2440 菌株是通過去除 pWW0 甲苯分解 (TOL) 質(zhì)粒而產(chǎn)生的。 主要用途： 用作模型生物宿主，用于重組 DNA 實驗、工業(yè)生物生產(chǎn)和環(huán)境生物修復(fù)項目。 形態(tài)： 它是革蘭氏陰性、桿狀細(xì)菌。 新陳代謝： 具有高度的多功能性；它擁有利用多種碳源（包括許多復(fù)雜的芳香族化合物）的代謝途徑。 氧氣需求： 它是專性需氧菌，生長需要氧氣。 最適溫度： 它是中溫生物，在 30°C 時生長最佳。 生物安全級別： 已被 NIH 認(rèn)證為安全的宿主-載體生物安全 1 級 (HV1) 系統(tǒng)，適合在標(biāo)準(zhǔn) P1 或 ML1 實驗室環(huán)境中使用。 研究應(yīng)用 無致病性： 它缺乏毒力因子（如在相關(guān)病原體銅綠假單胞菌中發(fā)現(xiàn)的 III 型分泌系統(tǒng)），這是其被認(rèn)證為安全的重組 DNA 工作實驗室菌株的關(guān)鍵原因。 代謝多功能性： 它能夠耐受和代謝多種有毒化學(xué)物質(zhì)和復(fù)雜碳源，非常適合將工業(yè)廢物轉(zhuǎn)化為有價值的產(chǎn)品（生物修復(fù)/生物催化）。 遺傳易操作性： 已經(jīng)開發(fā)了大量的遺傳工具（質(zhì)粒、轉(zhuǎn)座子）用于其操作，使其成為合成生物學(xué)應(yīng)用的有效“底盤”生物。 培養(yǎng)條件 培養(yǎng)基： 標(biāo)準(zhǔn)的 Luria–Bertani (LB) 肉湯或瓊脂平板通常用于普通實驗室培養(yǎng)。當(dāng)研究人員需要補充特定的碳源（如檸檬酸鹽或葡萄糖）時，可以使用最小培養(yǎng)基（例如 M9 最小鹽培養(yǎng)基）。 溫度： 培養(yǎng)物應(yīng)在 30°C 下生長以獲得最佳生長速度。 通氣： 需要足夠的通氣和有氧條件才能生長。 BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

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