BioVector? NTCC? BWP17 致病性酵母菌白色念珠菌BioVector? (Candida albicans)

The BWP17 strain is a widely used laboratory strain of the pathogenic yeast Candida albicans. It is a derivative of the well-characterized clinical isolate SC5314 and is an essential tool for molecular genetic studies due to specific auxotrophic markers that allow for highly efficient gene manipulation and selection processes using methods like Ura-blaster. Description and Characteristics Organism: Candida albicans. Strain Designation: BWP17; it is also cataloged as MYA-2543 by the ATCC. Origin: Derived through genetic manipulation of the parental SC5314 clinical isolate. Primary Use: A workhorse strain for gene deletion (knockout) and gene complementation studies in C. albicans research. Morphology: This organism is a dimorphic fungus, meaning it can grow in two forms: as budding yeast (blastospores) or as filamentous hyphae. Biosafety Level: Typically handled under BSL-1 or BSL-2 safety guidelines, as C. albicans is a common human pathogen. Genetic Features (Auxotrophies) The BWP17 strain is defined by homozygous deletions in three key genes, meaning it cannot produce these nutrients itself and requires them in the culture medium: ura3::λimm434/ura3::λimm434: Requires supplementation with uracil (or uridine). his1::hisG/his1::hisG: Requires supplementation with histidine. arg4::hisG/arg4::hisG: Requires supplementation with arginine. Culture and Handling Culture Medium: General Growth: YPAD (Yeast Peptone Dextrose medium supplemented with adenine) is standard rich media. Selection: Synthetic Complete (SC) media lacking specific amino acids (e.g., SC -His, SC -Arg) are used to select for organisms that have taken up a complementing plasmid. Growth Temperature: Optimal growth in the yeast form occurs at 30°C. Hyphal Induction: To study the pathogenic filamentous (hyphal) form of growth, cells are typically shifted to 37°C in specific inducing media (e.g., YPD + 5% Fetal Bovine Serum). BWP17 菌株是致病性酵母菌白色念珠菌 (Candida albicans) 的一種廣泛使用的實驗室菌株。它是經(jīng)過充分表征的臨床分離株 SC5314 的衍生物，由于具有多個營養(yǎng)缺陷型標記，可以通過 Ura-blaster 等方法實現(xiàn)高效的基因操作和篩選過程，因此是分子遺傳學研究的重要工具。 描述和特征 生物體： Candida albicans (白色念珠菌)。 菌株名稱： BWP17；在 ATCC 中也編目為 MYA-2543。 來源： 通過對親代 SC5314 臨床分離株進行基因操作而衍生。 主要用途： 是白色念珠菌研究中進行基因缺失（敲除）和基因互補研究的主力菌株。 形態(tài)： 這種生物體是一種雙態(tài)性真菌，意味著它可以以兩種形式生長：出芽酵母菌（芽生孢子）或絲狀菌絲體。 生物安全級別： 由于白色念珠菌是常見的人類病原體，通常在 BSL-1 或 BSL-2 安全指南下進行處理。 遺傳特征（營養(yǎng)缺陷型） BWP17 菌株的定義是三個關鍵生物合成基因存在純合缺失，這意味著它無法自行產(chǎn)生這些營養(yǎng)物質(zhì)，需要在培養(yǎng)基中額外補充： ura3::λimm434/ura3::λimm434： 需要補充尿嘧啶（或尿苷）。 his1::hisG/his1::hisG： 需要補充組氨酸。 arg4::hisG/arg4::hisG： 需要補充精氨酸。 培養(yǎng)和處理 培養(yǎng)基： 通用培養(yǎng)基： YPAD（補充腺嘌呤的酵母蛋白胨葡萄糖培養(yǎng)基）是標準的富集培養(yǎng)基。 最小/選擇性培養(yǎng)基： 缺乏特定氨基酸的合成完全培養(yǎng)基 (SC)（例如 SC -His、SC -Arg）用于篩選攜帶互補質(zhì)粒的轉化體。 生長溫度： 酵母菌形式的最佳生長溫度為 30°C。 菌絲誘導： 為了研究致病性絲狀（菌絲）生長形式，通常將細胞轉移到 37°C 的特定誘導培養(yǎng)基（例如 YPD + 5% 胎牛血清）中孵育。 BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

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