BioVector? NTCC? pRT802大腸桿菌鏈霉菌整合型和穿梭載體比他

The pRT802 plasmid is a specialized integrative expression vector primarily designed for use in Streptomyces species of bacteria, which are important for producing antibiotics. It allows researchers to insert genes into the bacterial genome at a specific, stable location. Key Features Vector Type: Integrative vector and shuttle vector (E. coli - Streptomyces). Host Organisms: Used for cloning in Escherichia coli and for functional use in a wide range of Streptomyces strains. Bacterial Resistance: Confers kanamycin resistance (typically used at 50 μg/mL). Integration System: Contains the site-specific integration machinery (integrase gene and attP attachment site) from the temperate phage φBT1. Origins of Replication: ColE1 origin (pBR322oriF): Allows the plasmid to replicate and be maintained in E. coli. It cannot replicate autonomously in Streptomyces. Instead, it integrates into the Streptomyces genome at the φBT1 attB site. Other Features: Includes a multiple cloning site (MCS) for inserting a gene of interest and an origin of conjugative transfer (oriT), which facilitates its transfer via tri-parental mating into Streptomyces. Usage in Research The pRT802 vector is commonly used in Streptomyces research for several applications: Gene Expression: To drive the expression of cloned genes within the Streptomyces host. Complementation: To reintroduce a functional copy of a gene into a mutant strain. Strain Marking: To produce stably marked strains for experimental purposes. Gene Deletion/Targeting: It is used as a backbone for constructing plasmids that facilitate gene knockout experiments via homologous recombination. pRT802 質(zhì)粒是一種特殊的整合表達載體，主要設計用于鏈霉菌屬 (Streptomyces) 細菌，該菌屬因產(chǎn)生抗生素而非常重要。它允許研究人員將基因插入細菌基因組的特定穩(wěn)定位置。 主要特征 載體類型： 整合型載體和穿梭載體（大腸桿菌 - 鏈霉菌）。 宿主生物體： 用于在大腸桿菌中進行克隆，并用于多種鏈霉菌菌株中發(fā)揮功能。 細菌抗性： 賦予卡那霉素耐藥性（通常使用 50 μg/mL）。 整合系統(tǒng)： 包含來自溫和噬菌體 φBT1 的位點特異性整合機制（整合酶基因和 attP 附著位點）。 復制起點： ColE1 起點 (pBR322oriF)： 允許質(zhì)粒在大腸桿菌中復制和維持。 它不能在鏈霉菌中自主復制。相反，它通過 φBT1 attB 位點整合到鏈霉菌基因組中。 其他特征： 包括一個多克隆位點 (MCS) 用于插入目標基因，以及一個接合轉(zhuǎn)移起點 (oriT)，便于通過三親交配將其轉(zhuǎn)移到鏈霉菌中。 研究用途 pRT802 載體通常用于鏈霉菌研究中的多種應用： 基因表達： 驅(qū)動克隆基因在鏈霉菌宿主中的表達。 互補： 將功能性基因拷貝重新引入突變菌株中。 菌株標記： 用于生產(chǎn)穩(wěn)定的標記菌株以用于實驗目的。 基因刪除/靶向： 它被用作構(gòu)建質(zhì)粒的骨架，通過同源重組促進基因敲除實驗。 BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

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