BioVector? NTCC? A549/DDP人肺腺癌細(xì)胞株順鉑耐藥亞株

The A549/DDP cell line is a cisplatin-resistant subline of the human lung adenocarcinoma A549 cell line. It was developed through continuous exposure to increasing concentrations of cisplatin (DDP) and is a critical model for studying mechanisms of chemotherapy resistance in lung cancer research. Key Characteristics Organism: Homo sapiens (Human) Tissue: Lung (pulmonary adenocarcinoma) Disease: Non-small cell lung cancer (NSCLC) Morphology: Epithelial-like, adherent monolayer cells, often noted to exhibit a more fibroblastic morphology compared to the parental A549 cells. Growth Properties: Adherent. Key Trait: Exhibits significant resistance to cisplatin-induced cell death; the IC50 value is significantly higher than that of the parental A549 line. Biosafety Level: Typically BSL-1. Research Applications The A549/DDP cell line is primarily used to: Investigate the biochemical pathways and mechanisms that confer resistance to cisplatin (e.g., Nrf2-mediated defenses, c-met pathway, increased autophagy). Screen for new drugs or drug combinations that can reverse resistance and sensitize the cells to cisplatin treatment. Culture Conditions Culture Medium: RPMI 1640 medium supplemented with 10% Fetal Bovine Serum (FBS) and stable L-glutamine (2.0 mM) is commonly used. Maintenance: To maintain the drug-resistant phenotype, the cells are typically cultured in medium containing a specific concentration of DDP (e.g., 10μM or 1μM, depending on the specific protocol/supplier). This cisplatin is often withdrawn for a few generations immediately before experiments are conducted. Growth Temperature: 37°C. CO? Concentration: 5% CO? in a humidified incubator. Subculture Routine: Wash cells with PBS lacking calcium and magnesium. Detachment is typically done using Accutase or trypsin/EDTA solution. Subcultivate when 70-80% confluent. A549/DDP 細(xì)胞系是人肺腺癌 A549 細(xì)胞系的順鉑耐藥亞株。它是通過持續(xù)暴露于濃度遞增的順鉑 (DDP) 中而培養(yǎng)出來的，是肺癌研究中用于研究化療耐藥機(jī)制的關(guān)鍵模型。 主要特征 生物體： Homo sapiens (人) 組織： 肺（肺腺癌） 疾?。?非小細(xì)胞肺癌 (NSCLC) 形態(tài)： 上皮樣，貼壁單層細(xì)胞，通常被描述為與親代 A549 細(xì)胞相比具有更強(qiáng)的成纖維細(xì)胞樣形態(tài)。 生長特性： 貼壁生長。 關(guān)鍵特性： 對順鉑誘導(dǎo)的細(xì)胞死亡表現(xiàn)出顯著抵抗力；IC50 值遠(yuǎn)高于親代 A549 細(xì)胞系。 生物安全級別： 通常為 BSL-1。 研究應(yīng)用 A549/DDP 細(xì)胞系主要用于： 研究賦予順鉑耐藥性的生化途徑和機(jī)制（例如，Nrf2 介導(dǎo)的防御、c-met 通路、自噬增加）。 篩選可以逆轉(zhuǎn)耐藥性并使細(xì)胞對順鉑治療敏感的新藥物或藥物組合。 培養(yǎng)條件 培養(yǎng)基： 通常使用補(bǔ)充 10% 胎牛血清 (FBS) 和穩(wěn)定 L-谷氨酰胺 (2.0 mM) 的 RPMI 1640 培養(yǎng)基。 維護(hù)： 為了維持耐藥表型，通常將細(xì)胞培養(yǎng)在含有特定濃度 DDP（例如 10μM 或 1μM，取決于具體的方案/供應(yīng)商）的培養(yǎng)基中。在進(jìn)行實(shí)驗(yàn)前，通常會(huì)立即停止使用順鉑幾代。 生長溫度： 37°C。 CO? 濃度： 在 37°C、5% CO? 的加濕培養(yǎng)箱中培養(yǎng)。 傳代程序： 用不含鈣和鎂的 PBS 洗滌細(xì)胞。 通常使用 Accutase 或胰蛋白酶/EDTA 溶液進(jìn)行細(xì)胞消化。 當(dāng)細(xì)胞匯合度達(dá)到 70-80% 時(shí)進(jìn)行傳代。 BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

Supplier來源:BioVector NTCC Inc.
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Website網(wǎng)址: http://www.nedfriskphoto.com