BioVector? NTCC? pSRK廣宿主表達載體BioVector?質(zhì)粒

The pSRK plasmid family consists of broad-host-range expression vectors used for tightly regulated gene expression in diverse Gram-negative bacteria. These plasmids are popular because they can function in a wide range of bacterial species and feature a tightly controlled, IPTG-inducible promoter system. Description and Characteristics Vector Type: Broad-host-range expression vector, meaning it is compatible with many different types of Gram-negative bacteria. Backbone Origin: The design is based on the pBBR1 replicon, which is a stable origin of replication. Host Range: Effective in a wide variety of bacteria, including Agrobacterium tumefaciens, Pseudomonas fluorescens, Rhizobium leguminosarum, and Caulobacter crescentus. Expression System: Uses an IPTG-inducible lac promoter system (lacIq-Plac). This system keeps the target gene expression turned off until the inducer chemical, IPTG, is added to the medium. Selection Marker: The specific antibiotic resistance marker varies depending on the variant of the pSRK plasmid. Common Variants pSRKKm: Confers resistance to kanamycin (typically used at 25-50 μg/mL). pSRKGm: Confers resistance to gentamicin. pSRKTc: Confers resistance to tetracycline. Usage and Culture Conditions Host Organisms: Can be propagated in standard E. coli cloning strains like DH5alpha, but is engineered for functional use in other diverse Gram-negative hosts. Culture Medium: Luria–Bertani (LB) medium is standard for E. coli. The specific requirements for different bacterial hosts may vary. Temperature: Typically cultured at 37°C for E. coli, but some specific hosts like Rhodococcus opacus PD630 grow optimally at 30°C. Induction: Gene expression is switched "on" by adding the chemical IPTG (Isopropyl β-D-1-thiogalactopyranoside) to the actively growing culture medium. pSRK 質(zhì)粒家族包含廣宿主范圍的表達載體，用于在多種革蘭氏陰性細菌中進行嚴格調(diào)控的基因表達。它們之所以受歡迎，是因為它們能夠在多種細菌物種中發(fā)揮作用，并且具有嚴格控制的、IPTG 誘導型啟動子系統(tǒng)。 描述和特征 載體類型： 廣宿主范圍表達載體，意味著它與許多不同類型的革蘭氏陰性細菌兼容。 骨架來源： 該設(shè)計基于 pBBR1 復制子，這是一個穩(wěn)定的復制起點。 宿主范圍： 在多種細菌中有效，包括根癌農(nóng)桿菌 (Agrobacterium tumefaciens)、熒光假單胞菌 (Pseudomonas fluorescens)、豌豆根瘤菌 (Rhizobium leguminosarum) 和新月柄桿菌 (Caulobacter crescentus)。 表達系統(tǒng)： 使用 IPTG 誘導型 lac 啟動子系統(tǒng) (lacIq-Plac)。該系統(tǒng)使目標基因的表達保持關(guān)閉狀態(tài)，直到將誘導劑化學物質(zhì) IPTG 添加到培養(yǎng)基中。 篩選標記： 特異的抗生素耐藥性標記根據(jù) pSRK 質(zhì)粒的不同變體而異。 常見變體 pSRKKm： 賦予卡那霉素耐藥性（通常使用 25-50 μg/mL）。 pSRKGm： 賦予慶大霉素耐藥性。 pSRKTc： 賦予四環(huán)素耐藥性。 用途和培養(yǎng)條件 宿主生物體： 可以在標準大腸桿菌克隆菌株（如 DH5alpha）中繁殖，但設(shè)計用于在其他多種革蘭氏陰性宿主中發(fā)揮功能。 培養(yǎng)基： Luria–Bertani (LB) 培養(yǎng)基是大腸桿菌的標準培養(yǎng)基。不同細菌宿主的具體要求可能有所不同。 溫度： 大腸桿菌通常在 37°C 培養(yǎng)，但某些特定宿主（如抗酸羅氏菌 Rhodococcus opacus PD630）在 30°C 下生長最佳。 誘導： 通過向積極生長的培養(yǎng)基中添加化學物質(zhì) IPTG（異丙基-β-D-硫代半乳糖苷）來開啟基因表達。 BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com