BioVector? NTCC? MM294大腸桿菌 (E. coli) 菌株高產(chǎn)量純凈質(zhì)粒 DNA生產(chǎn)菌種BioVector?

The BioVector? E. coli MM294 strain is a widely used laboratory strain of Escherichia coli, derived from the K-12 lineage. It is primarily employed in cloning and plasmid DNA production because of its high efficiency in taking up foreign DNA (transformability) and its ability to yield high quantities of pure plasmid DNA. Description and Characteristics Organism: Escherichia coli. Strain Designation: K12 MM294, or simply MM294. Origin: It originated from the laboratory of Matthew Meselson. Primary Use: A robust host for the transformation and propagation of plasmids in molecular biology workflows. Phenotype: It has a standard wild-type appearance. Plasmid Status: The strain is free of any native plasmids. Biosafety Level: It is typically classified as Biosafety Level 1 (BSL-1). Key Genetic Features (Genotype) The specific genetic modifications of MM294 provide functional advantages for cloning: F?: This means the strain is a recipient during bacterial conjugation, making it unable to act as a donor of genetic material via conjugation. endA1 mutation: This mutation inactivates the cell's internal endonuclease I enzyme. The absence of this enzyme is crucial because it prevents the degradation of delicate plasmid DNA during the purification process, leading to better yields and higher quality DNA. hsdR17 (rK?mK?) mutation: This affects the restriction-modification system. The strain cannot cut (restrict) foreign DNA that lacks specific methylation patterns (common in DNA from other organisms like eukaryotes), which vastly improves cloning efficiency. supE44 / glnV44 suppressor mutation: This helps ensure that the entire protein sequence encoded by a plasmid gene is translated correctly, by reading through certain premature stop codons. thi-1: This indicates a nutritional requirement for thiamine (Vitamin B1) in the growth medium. λ? (lambda-minus): The strain lacks the lambda prophage. Culture and Handling Culture Medium: Standard Luria–Bertani (LB) broth or agar plates are the standard for cultivation. Growth Conditions: Cultures are grown aerobically. Temperature: Incubation is typically performed at 37°C. Storage: The strain can be maintained on agar slants at +4°C for 1-2 weeks. For long-term preservation, it should be frozen in a glycerol stock solution and stored at -80°C. BioVector? MM294 大腸桿菌 (E. coli) 菌株是一種廣泛使用的實(shí)驗(yàn)室菌株，源自 K-12 譜系。它主要用于克隆和質(zhì)粒 DNA 的生產(chǎn)，因?yàn)槠渚哂休^高的外源 DNA 攝取效率（轉(zhuǎn)化能力）和在質(zhì)粒小量制備過程中產(chǎn)生高產(chǎn)量純凈質(zhì)粒 DNA 的能力。 描述和特征 生物體： Escherichia coli (大腸桿菌)。 菌株名稱： K12 MM294，或簡稱 MM294。 來源： 源自 Matthew Meselson 的實(shí)驗(yàn)室。 主要用途： 在分子生物學(xué)工作流程中，用作質(zhì)粒轉(zhuǎn)化和增殖的穩(wěn)定宿主。 表型： 具有標(biāo)準(zhǔn)的野生型外觀。 質(zhì)粒狀態(tài)： 該菌株不含任何內(nèi)源質(zhì)粒。 生物安全級(jí)別： 通常歸類為生物安全 1 級(jí) (BSL-1)。 關(guān)鍵遺傳特征（基因型） MM294 的特定基因修飾提供了克隆的功能優(yōu)勢： F?： 這意味著該菌株在細(xì)菌接合過程中是受體，因此不能通過接合作用充當(dāng)遺傳物質(zhì)的供體。 endA1 突變： 該突變使細(xì)胞內(nèi)部的核酸內(nèi)切酶 I 失活。這種酶的缺失至關(guān)重要，因?yàn)樗梢苑乐乖诩兓^程中質(zhì)粒 DNA 被降解，從而提高產(chǎn)量和 DNA 純度。 hsdR17 (rK?mK?) 突變： 這會(huì)影響限制-修飾系統(tǒng)。該菌株不能切割（限制）缺乏特定甲基化模式的外源 DNA（在來自真核生物等其他生物體的 DNA 中很常見），這極大地提高了克隆效率。 supE44 / glnV44 抑制突變： 這有助于確保質(zhì)粒基因編碼的整個(gè)蛋白質(zhì)序列被正確翻譯，通過讀取某些提前終止密碼子來實(shí)現(xiàn)。 thi-1： 這表明在生長培養(yǎng)基中需要硫胺素（維生素 B1）作為營養(yǎng)補(bǔ)充。 λ?（lambda 陰性）： 該菌株不含 lambda 原噬菌體。 培養(yǎng)和處理 培養(yǎng)基： 標(biāo)準(zhǔn)的 Luria–Bertani (LB) 肉湯或瓊脂平板是培養(yǎng)的標(biāo)準(zhǔn)培養(yǎng)基。 生長條件： 培養(yǎng)物需在有氧條件下生長。 溫度： 通常在 37°C 下孵育。 儲(chǔ)存： 菌株可以在 +4°C 的瓊脂斜面上維持 1-2 周。為了長期保存，應(yīng)冷凍在甘油儲(chǔ)備液中，并儲(chǔ)存在 -80°C。 BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號(hào):1843439339 網(wǎng)址 www.nedfriskphoto.com

Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com