BioVector? NTCC? NK5449細(xì)菌接合實驗受體大腸桿菌菌株BioVector?

NK5449 strain" refers to a specific strain of Escherichia coli (E. coli) widely used in molecular biology and environmental science. It functions primarily as a recipient strain in laboratory experiments designed to study horizontal gene transfer—the process by which bacteria share genetic material, such as antibiotic resistance genes. Description and Characteristics Organism: Escherichia coli. Strain Designation: NK5449. Primary Use: Acts as a recipient in bacterial conjugation experiments. Key Resistance Markers: The most important characteristic of NK5449 is its intrinsic, high-level resistance to two specific antibiotics: Rifampicin (>160 mg/L) and Nalidixic Acid (>50 mg/L). This dual resistance allows researchers to easily select this strain in co-culture experiments. Plasmid Status: The strain is "plasmid-free," meaning it does not naturally contain any native plasmids, which is crucial for reliably accepting new plasmids during experiments. Supplier Information: It is cataloged by the BioVector NTCC Inc. under catalog number BioVector?-1.1437. Biosafety Level: It is typically handled under BSL-1 or BSL-2 safety levels, depending on specific experimental procedures and local laboratory safety guidelines. Usage in Research In a typical lab experiment, the NK5449 strain is mixed with a "donor" strain (e.g., E. coli HB101 containing a plasmid like RP4). By plating the mixture on agar containing all four antibiotics (kanamycin, tetracycline, rifampicin, and nalidixic acid), only the bacteria that successfully received the donor plasmid and are the resistant NK5449 strain can survive. This method is used to: Evaluate the efficiency of how plasmids transfer between bacteria. Assess how environmental factors (like microplastics or pollutants) affect the spread of antibiotic resistance. Culture and Handling Culture Medium: Standard Luria–Bertani (LB) broth or agar plates work well for growth. Selection: Appropriate concentrations of rifampicin and/or nalidixic acid must be added to the media if selection pressure is required. Temperature: Cultured aerobically at 37°C. NK5449 株系指的是大腸桿菌 (E. coli) 的一個特定菌株，廣泛應(yīng)用于分子生物學(xué)和環(huán)境科學(xué)領(lǐng)域。它主要在實驗室實驗中用作受體菌株，用于研究抗生素耐藥基因通過接合作用進(jìn)行水平轉(zhuǎn)移的過程。 描述和特征 生物體： Escherichia coli (大腸桿菌)。 菌株名稱： NK5449。 主要用途： 在細(xì)菌接合實驗中充當(dāng)受體。 關(guān)鍵耐藥標(biāo)記： NK5449 最重要的特征是其對兩種特定抗生素具有固有的高水平耐藥性：利福平 (>160 mg/L) 和 萘啶酸 (>50 mg/L)。這種雙重耐藥性使研究人員可以在共培養(yǎng)實驗中輕松篩選該菌株。 質(zhì)粒狀態(tài)： 該菌株“不含質(zhì)?！?，意味著其天然不含任何內(nèi)源質(zhì)粒，這對于在實驗中可靠地接受新質(zhì)粒至關(guān)重要。 供應(yīng)商信息： BioVector?-1.1437。 生物安全級別： 根據(jù)具體的實驗程序和當(dāng)?shù)貙嶒炇野踩改?，通常?BSL-1 或 BSL-2 安全級別下處理。 研究用途 在典型的實驗室實驗中，NK5449 菌株與供體菌株（例如，含有 RP4 質(zhì)粒的大腸桿菌 HB101）混合。通過在含有所有四種抗生素（來自供體質(zhì)粒的卡那霉素/四環(huán)素，以及來自受體 NK5449 菌株的利福平/萘啶酸）的瓊脂平板上培養(yǎng)混合物，只有成功接收供體質(zhì)粒并且是耐藥 NK5449 菌株的細(xì)菌才能存活。這種方法用于： 評估質(zhì)粒在細(xì)菌之間轉(zhuǎn)移的效率。 評估環(huán)境因素（如微塑料或污染物）如何影響抗生素耐藥性的傳播。 培養(yǎng)和處理 培養(yǎng)基： 標(biāo)準(zhǔn)的 Luria–Bertani (LB) 肉湯或瓊脂平板適合其生長。 篩選： 如果需要篩選壓力，必須在培養(yǎng)基中添加適當(dāng)濃度的利福平和/或萘啶酸。 溫度： 在 37°C 的好氧培養(yǎng)箱中培養(yǎng)。 BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

Supplier來源:BioVector NTCC Inc.
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Website網(wǎng)址: http://www.nedfriskphoto.com