BioVector? NTCC? pPROBE-gfp\(ASV\) 廣宿主范圍的啟動子探針載體BioVector?

ThinkingSearching The pPROBE-gfp\(ASV\) plasmid is a broad-host-range promoter-probe vector used as a transcriptional reporter system in various Gram-negative bacteria. It contains an unstable variant of the green fluorescent protein (Gfp) as a reporter gene to study real-time, dynamic gene expression.?Plasmid Details?Vector Name: pPROBE-gfp\(ASV\) (often referred to as pPROBE'-gfp\(ASV\))Vector Type: Broad-host-range promoter-probe vectorDeposited by: Steven Lindow (UC Davis)Bacterial Resistance: Kanamycin (50 μg/mL)Backbone Origin: pBBR1 oriV (origin of replication), allowing replication in a wide range of bacterial species.Length: Approximately 7385 base pairs (bp).?Key Components?Reporter Gene: gfp\(ASV\) gene.Gfp\(ASV\): This is a variant of the Green Fluorescent Protein with a short half-life (unstable). The "ASV" tag targets the protein for degradation by the host cell's lon protease, allowing for the detection of rapid changes in promoter activity.Promoter-Probe Cassette: A multicloning site (MCS) is located upstream of the gfp gene. Researchers ligate specific promoter sequences into this MCS. The expression of the Gfp reporter is then entirely dependent on the inserted promoter's activity.Transcriptional Terminators: Strong transcriptional terminators flank the reporter cassette to prevent transcription from external or vector-borne promoters from interfering with the assay.?Usage and Application?Function: Used to quantify gene expression driven by an inserted promoter sequence in various Gram-negative bacteria.Assay Method: Promoter activity can be quantified using flow cytometry or colony assays by measuring the fluorescence signals.Advantage: The unstable Gfp variant provides a more immediate and dynamic measure of promoter activity compared to stable Gfp variants, which accumulate over time. pPROBE-gfp\(ASV\) 質(zhì)粒是一種廣宿主范圍的啟動子探針載體，用作多種革蘭氏陰性細(xì)菌中的轉(zhuǎn)錄報告系統(tǒng)。它含有一個不穩(wěn)定的綠色熒光蛋白 (Gfp) 變體作為報告基因，用于研究實(shí)時、動態(tài)的基因表達(dá)。?質(zhì)粒詳細(xì)信息?載體名稱： pPROBE-gfp\(ASV\)（通常稱為 pPROBE'-gfp\(ASV\)）載體類型： 廣宿主范圍啟動子探針載體提供者： Steven Lindow (UC Davis)細(xì)菌抗性： 卡那霉素 (50 μg/mL)骨架來源： pBBR1 oriV（復(fù)制起點(diǎn)），允許在多種細(xì)菌物種中復(fù)制。長度： 約 7385 個堿基對 (bp)。?主要組成部分?報告基因： gfp\(ASV\) 基因。Gfp\(ASV\)： 這是綠色熒光蛋白的一個變體，半衰期短（不穩(wěn)定）。"ASV" 標(biāo)簽引導(dǎo)該蛋白被宿主細(xì)胞的 lon 蛋白酶降解，從而可以檢測啟動子活性的快速變化。啟動子探針盒： 在 gfp 基因上游有一個多克隆位點(diǎn) (MCS)。研究人員將特定的啟動子序列連接到此 MCS 中。Gfp 報告基因的表達(dá)完全取決于插入的啟動子的活性。轉(zhuǎn)錄終止子： 強(qiáng)的轉(zhuǎn)錄終止子位于報告基因盒兩側(cè)，以防止來自外部或載體上的啟動子干擾檢測。?用途和應(yīng)用?功能： 用于量化由插入的啟動子序列驅(qū)動的基因在各種革蘭氏陰性細(xì)菌中的表達(dá)。檢測方法： 啟動子活性可以通過流式細(xì)胞術(shù)或菌落分析（通過測量熒光信號）來量化。優(yōu)勢： 與隨時間積累的穩(wěn)定 Gfp 變體相比，不穩(wěn)定的 Gfp 變體提供了更即時、動態(tài)的啟動子活性測量。 BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

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