BioVector? NTCC? GIST-48貼壁人胃腸道間質(zhì)瘤 (GIST) 細(xì)胞系NTCC?

The GIST-48 cell line is an adherent human gastrointestinal stromal tumor (GIST) cell line established from a tumor that progressed during imatinib therapy. It is a key model for studying drug resistance due to its specific combination of KIT gene mutations. Key Characteristics Organism: Homo sapiens (Human) Disease: Gastrointestinal Stromal Tumor (GIST), specifically imatinib-resistant. Growth Properties: Adherent monolayer. Morphology: Spindle-shaped/epithelial-like cells. Key Mutations: The line is characterized by two KIT mutations: Primary Mutation: Homozygous KIT exon 11 V560D point mutation. Secondary (Resistance) Mutation: Heterozygous KIT exon 17 D820A missense mutation. Immunomarker: Expresses c-kit (CD117) and CD34. Derived Subline: The GIST48B subline was derived from GIST-48 after drug pressure, resulting in nearly undetectable KIT expression. Culture Conditions Culture Medium: F10 medium supplemented with 15% Fetal Bovine Serum (FBS), serum extender, and bovine pituitary extract. Other protocols use a 50:50 mixture of Dulbecco's Modified Eagle's Medium/Ham's F-12 (DMEM/F-12) with 15% FBS. Growth Temperature: 37°C. CO? Concentration: 5% CO? in a humidified incubator. Subculture Routine: GIST-48 cells are typically incubated for 120 hours in experiments, suggesting a slow growth rate similar to other GIST lines. They are often split 1:3 when subculturing. GIST-48 細(xì)胞系是一種貼壁人胃腸道間質(zhì)瘤 (GIST) 細(xì)胞系，建立自一名在伊馬替尼治療期間出現(xiàn)疾病進展的患者腫瘤。由于其 KIT 基因突變的特定組合，它是研究耐藥性的重要模型。 主要特征 生物體： Homo sapiens (人) 疾病： 胃腸道間質(zhì)瘤 (GIST)，具體表現(xiàn)為伊馬替尼耐藥性。 生長特性： 貼壁單層生長。 形態(tài)： 紡錘形/上皮樣細(xì)胞。 關(guān)鍵突變： 該細(xì)胞系具有兩個 KIT 突變： 原發(fā)突變： KIT 外顯子 11 純合 V560D 點突變。 繼發(fā)（耐藥）突變： KIT 外顯子 17 雜合 D820A 錯義突變。 免疫標(biāo)記： 表達 c-kit (CD117) 和 CD34。 衍生亞系： GIST48B 亞系是在藥物壓力下從 GIST-48 衍生而來，導(dǎo)致幾乎檢測不到 KIT 表達。 培養(yǎng)條件 培養(yǎng)基： F10 培養(yǎng)基，補充 15% 胎牛血清 (FBS)、血清延伸物和牛垂體提取物。其他方案使用 Dulbecco's Modified Eagle's Medium/Ham's F-12 (DMEM/F-12) 的 50:50 混合物和 15% FBS。 生長溫度： 37°C。 CO? 濃度： 在 37°C、5% CO? 的恒溫培養(yǎng)箱中培養(yǎng)。 傳代程序： GIST-48 細(xì)胞在實驗中通常培養(yǎng) 120 小時，表明其生長速度較慢，類似于其他 GIST 細(xì)胞系。傳代時通常按 1:3 的比例稀釋。 BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心 電話:400-800-2947 工作QQ/微信同號:1843439339 網(wǎng)址 www.nedfriskphoto.com

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