BioVector? NTCC? pComb3XSS噬菌體展示技術(shù)表達(dá)載體BioVector? Filamentous phage display plasmid

基本信息

質(zhì)粒名稱(chēng): pComb3XSS BioVector?

質(zhì)粒類(lèi)型: 表達(dá)載體，噬菌體展示系統(tǒng)

宿主: 大腸桿菌（E. coli）

主要用途

用于 噬菌體展示技術(shù)（Phage Display）

蛋白質(zhì)-肽庫(kù)構(gòu)建

蛋白質(zhì)與抗體相互作用研究

高通量篩選結(jié)合位點(diǎn)

質(zhì)粒特性

復(fù)制起點(diǎn)（Ori）: ColE1

選擇標(biāo)記: 抗生素抗性（通常為氨芐青霉素）

融合標(biāo)簽: 可構(gòu)建噬菌體展示融合蛋白（通常為 pIII 蛋白）

多克隆位點(diǎn) (MCS): 允許插入目標(biāo)基因序列

表達(dá)信息

適合在 E. coli 中表達(dá)融合蛋白

可用于噬菌體展示文庫(kù)構(gòu)建

注意事項(xiàng)

遵守生物安全規(guī)范（通常 BSL-1）

儲(chǔ)存條件：-20℃ 長(zhǎng)期保存

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
電話(huà):400-800-2947
工作QQ/微信同號(hào):1843439339
網(wǎng)址 www.nedfriskphoto.com

General Information

Name: pComb3XSS BioVector?

Type: Filamentous phage display plasmid (phagemid)

Host Range: E. coli (commonly used strains compatible with phage display)

Primary Use:

Antibody library construction

Phage display of antibody fragments (scFv, Fab)

Screening and affinity selection of binding proteins

Backbone Features

Origin of Replication:

E. coli origin for high-copy plasmid maintenance

Filamentous phage origin for packaging into phage particles (requires helper phage)

Selectable Marker:

Antibiotic resistance gene for selection in E. coli (depends on vendor/source)

Multiple Cloning Site (MCS):

Supports insertion of antibody variable regions or other display constructs

Includes restriction sites designed for library construction (no sequences provided)

Display Region:

Gene fusion region enabling display on phage coat protein (commonly pIII)

Allows surface presentation of inserted antibody fragments

Stop/Start Configuration:

“SS” in pComb3XSS refers to an improved stop/start configuration enhancing expression and display efficiency

Expression / Display Characteristics

Supports expression of antibody fragments in a format suitable for phage pIII fusion

Compatible with helper phage systems for packaging

Designed for improved phage titers and display efficiency compared to earlier pComb vectors

Typical Applications

Construction of synthetic, immune, or semi-synthetic antibody libraries

Biopanning (phage selection)

Discovery of binders to proteins, peptides, glycans, small molecules, or cells

Research in immunology, structural biology, and directed evolution

Storage

Typically stored as purified plasmid DNA or as glycerol stocks

Maintain under standard DNA storage conditions (details vary by provider)

Biosafety Information

BSL-1 organism/plasmid for routine laboratory handling

Non-pathogenic phagemid requiring helper phage for packaging

Standard laboratory safety practices recommended
BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
電話(huà):400-800-2947
工作QQ/微信同號(hào):1843439339
網(wǎng)址 www.nedfriskphoto.com


Supplier來(lái)源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com