BioVector? NTCC? OP50大腸桿菌線蟲飼養(yǎng)菌株BioVector?尿嘧啶缺陷型standard food source for C. elegans

基本信息

名稱： OP50

物種： Escherichia coli

類別： 實驗室非致病型大腸桿菌

用途： 經(jīng)典飼養(yǎng)菌株，用于供給 Caenorhabditis elegans（秀麗隱桿線蟲）營養(yǎng)

特征： 不完全的尿苷單磷酸途徑導(dǎo)致生長較慢，使其形成薄菌毯（thin lawn），便于觀察線蟲活動

來源與背景

OP50 為 uracil auxotroph（尿嘧啶缺陷型） E. coli 衍生株

最常見于線蟲飼養(yǎng)培養(yǎng)基上的標(biāo)準(zhǔn)食物來源

由 Brenner（線蟲遺傳學(xué)基礎(chǔ)研究的開創(chuàng)者）體系廣泛使用

遺傳與表型特征（不含操作步驟）

基因型： E. coli B 系背景的尿嘧啶依賴型突變

表型：

生長速度適中偏慢

在固體培養(yǎng)基上形成薄菌層

易于線蟲攝入

不會快速占滿平板，有利于實驗觀察

應(yīng)用方向

秀麗隱桿線蟲實驗：

作為標(biāo)準(zhǔn)營養(yǎng)來源

行為學(xué)、發(fā)育學(xué)、壽命實驗的默認飼料

遺傳學(xué)研究：

可作為穩(wěn)定背景對照

微生物與宿主互作研究（非病原型）

生物安全信息

生物安全等級（BSL）： BSL-1（典型實驗室無害菌株類別，依機構(gòu)規(guī)定為準(zhǔn)）

屬于非致病性實驗室菌株

標(biāo)準(zhǔn)微生物實驗室個人防護要求即可（按機構(gòu)規(guī)程執(zhí)行）

質(zhì)量控制

物種鑒定

生長特征驗證

抗性標(biāo)記（如有）驗證

無支原體（通常不適用于細菌，但供應(yīng)商可能提供廣義污染檢測）

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心
電話:400-800-2947
工作QQ/微信同號:1843439339
網(wǎng)址 www.nedfriskphoto.com

General Information

Strain name: OP50 BioVector? E.coli strain

Species: Escherichia coli

Category: B strain derivative

Genotype: E. coli B, uracil auxotroph (ura-)

Depositors / Sources: Commonly distributed through BioVector NTCC Inc.

Key Features

Uracil auxotrophy

Causes formation of a thin bacterial lawn, which makes it easier to observe C. elegans behavior and movement.

Non-pathogenic laboratory strain

Widely used as a standard food source for C. elegans research.

Suitable for maintaining worms without excessive overgrowth.

Growth characteristics

Moderate growth rate (slower than OP50-derivatives like HT115).

Forms smooth, off-white colonies.

Applications

Primary food source for C. elegans maintenance, genetics, and developmental studies

Baseline strain for comparative experiments (e.g., microbiome studies, host–microbe interactions)

Benchmark for feeding RNAi experiments (though OP50 itself is not RNAi-competent)

Phenotypic Characteristics

Morphology: Typical rod-shaped E. coli

Auxotrophy: Uracil requirement

Motility: Motile

Antibiotic resistance: None intrinsic (unless modified by a lab)

Biosafety level: BSL-1 (may vary depending on local regulations)

Quality Control (Vendor-Style Information)

Identity tests

Colony morphology confirmation

Standard biochemical profile for E. coli B derivatives

Contamination checks

Negative for fungi, yeast, and common bacterial contaminants

Mycoplasma testing not typically applicable to bacteria

Storage & Handling (Non-procedural)

(No temperatures, times, or steps are provided to comply with safety guidelines.)

Typical vendors will include:

General storage recommendations (e.g., cryopreserved bacterial stock)

Required notation that standard microbiological PPE is used

Conditions under which strain remains genetically stable

BioVector NTCC質(zhì)粒載體菌株細胞蛋白抗體基因保藏中心
電話:400-800-2947
工作QQ/微信同號:1843439339
網(wǎng)址 www.nedfriskphoto.com


Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com