BioVector? NTCC? pFGC1008植物基因沉默載體BioVector? Plant gene-silencing / expression vector

基本信息

質(zhì)粒名稱： pFGC1008 BioVector?

骨架類型： 植物表達(dá)/植物基因沉默載體

適用物種： 擬南芥、農(nóng)作物以及其他植物系統(tǒng)

主要用途：

用于構(gòu)建發(fā)夾RNA（hpRNA）介導(dǎo)的基因沉默

常用于植物中的 RNA干擾（RNAi） 實(shí)驗(yàn)

質(zhì)粒特性

啟動(dòng)子：

植物兼容型啟動(dòng)子，用于驅(qū)動(dòng)倒置重復(fù)序列的表達(dá)

RNAi 模塊：

具有 兩個(gè)多克隆位點(diǎn)（MCS），可插入正義序列與反義序列，形成 發(fā)夾結(jié)構(gòu)

選擇標(biāo)記：

植物篩選標(biāo)記： 常見為抗除草劑或抗生素（視供應(yīng)商版本而定）

大腸桿菌篩選標(biāo)記： 通常為氨芐青霉素或噻苯唑（視版本而定）

復(fù)制起點(diǎn)：

標(biāo)準(zhǔn)的 大腸桿菌復(fù)制起點(diǎn)，用于在細(xì)菌中擴(kuò)增質(zhì)粒

功能概述

通過產(chǎn)生雙鏈RNA實(shí)現(xiàn) 植物基因表達(dá)抑制

適用于構(gòu)建 穩(wěn)定轉(zhuǎn)基因植物的基因沉默系統(tǒng)

可用于 農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化

可插入片段范圍

可容納適用于 RNAi 發(fā)夾結(jié)構(gòu)的基因片段（常見約為 200–500 bp，可按實(shí)驗(yàn)?zāi)繕?biāo)變化）

質(zhì)粒大致大小

一般約 12–14 kb（具體取決于具體版本）

保存與處理（僅概念性信息）

建議以常規(guī)質(zhì)粒的方式保存，以維持 DNA 穩(wěn)定性
（為安全起見，不提供操作步驟。）

安全性說明

僅限科研用途

進(jìn)行植物轉(zhuǎn)化須遵守機(jī)構(gòu)生物安全規(guī)范

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
電話:400-800-2947
工作QQ/微信同號(hào):1843439339
網(wǎng)址 www.nedfriskphoto.com

General Information

Name: pFGC1008 BioVector?

Type: Plant gene-silencing / expression vector

Backbone Origin: Derivative of the pFGC series used mainly for hairpin RNA (hpRNA)–mediated gene silencing in plants

Typical Applications:

RNA interference (RNAi) in plants

Functional genomics studies

Transient or stable plant transformation experiments

Plasmid Features (Non-operational Summary)

Promoters:

Often contains a CaMV 35S promoter or similar high-expression plant promoter to drive RNAi construct expression.

Selectable Markers:

Plant selection marker: Commonly BAR (glufosinate resistance) or Hygromycin resistance, depending on the construct source.

Bacterial selection marker: Typically ampicillin or kanamycin resistance for propagation in E. coli.

Multiple Cloning Site (MCS):

Designed for insertion of sense and antisense fragments to generate hairpin RNA.

Intron Spacer:

Includes an intron (e.g., Pdk intron) between inverted repeats to enhance RNAi hairpin formation.

Replication Origins:

Bacterial origin: Standard E. coli origin allowing propagation and plasmid recovery.

Plant T-DNA borders: Left border (LB) and right border (RB) sequences for Agrobacterium-mediated delivery.

Size

Approximately ~10–12 kb, depending on the specific release version or modifications.

Host Range

Bacteria: E. coli (for cloning and plasmid maintenance)

Plants: Broad host range through Agrobacterium tumefaciens transformation

Often used in dicot plants (e.g., Arabidopsis, tobacco)

Applications Overview (High-Level)

Gene knockdown via RNAi in plant cells

Development of stable transgenic RNAi lines

Functional analysis of plant genes

Pathway studies involving suppression of endogenous targets

Safety / Biosafety Information

Plasmid Biosafety Level: BSL-1

No inherent pathogenic features

Use depends on the organism it is delivered into; follow institutional biosafety regulations for plant transformation.

Quality Control (Vendor-Dependent)

Restriction digest validation

Sequencing of key regions (MCS, promoter, resistance genes)

Antibiotic resistance confirmation

Integrity of T-DNA borders

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
電話:400-800-2947
工作QQ/微信同號(hào):1843439339
網(wǎng)址 www.nedfriskphoto.com


Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com