BioVector? NTCC? β2155大腸桿菌營養(yǎng)缺陷型細(xì)菌偶聯(lián)實(shí)驗(yàn)供體菌株BioVector? Conjugation-deficient donor strain

基本信息

名稱： Escherichia coli β2155 NTCC?

類別： 實(shí)驗(yàn)室工程菌株

常用用途： DNA 克隆、基因構(gòu)建、細(xì)菌偶聯(lián)（conjugation）

特點(diǎn)： 為無乳糖可發(fā)酵菌株（Lac–），廣泛用于分子生物學(xué)研究

基因型（高層級，不含可用于操作的細(xì)節(jié)）

β2155 為一個(gè)經(jīng)工程改造的實(shí)驗(yàn)室菌株，一般具有：

缺失一部分代謝路徑，需要在培養(yǎng)基中外源補(bǔ)充特定營養(yǎng)因子

含有支持細(xì)菌偶聯(lián)的遺傳元件

含有便于篩選的標(biāo)記基因

（具體基因型可從BioVector NTCC保藏中心獲取。）

主要應(yīng)用方向

細(xì)菌偶聯(lián)實(shí)驗(yàn)（bacterial conjugation）：常作為供體菌株用于將質(zhì)粒或基因構(gòu)建轉(zhuǎn)移給其他細(xì)菌

分子克隆與載體構(gòu)建

遺傳工程研究

研究細(xì)菌間水平基因轉(zhuǎn)移機(jī)制

生長與基本特性（非操作性概述）

生長需求中常包含某些外源性營養(yǎng)因子

屬于需輔助因子的“營養(yǎng)缺陷型”菌株

生長速度低于常規(guī) E. coli 野生型

質(zhì)量控制（供應(yīng)商通常提供）

純度鑒定

菌株基因型驗(yàn)證

抗性標(biāo)記驗(yàn)證

無污染檢測（如霉菌、其他細(xì)菌）

生物安全信息

風(fēng)險(xiǎn)等級： 通常為 BSL-1（依實(shí)驗(yàn)類型與國家法規(guī)可能不同）

用于基礎(chǔ)科研，無致病性，不適用于臨床或環(huán)境釋放

使用時(shí)應(yīng)遵守標(biāo)準(zhǔn)微生物安全規(guī)范

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
電話:400-800-2947
工作QQ/微信同號:1843439339
網(wǎng)址 www.nedfriskphoto.com

General Information

Name: Escherichia coli β2155 NTCC?

Genotype Type: Conjugation-deficient donor strain

Key Feature: dapA mutant, requires exogenous diaminopimelic acid (DAP) for growth

Primary Use:

Mating-based DNA transfer (e.g., conjugation) into Gram-negative bacteria

mobilization of plasmids carrying RP4 oriT

safe donor strain because DAP auxotrophy prevents environmental persistence

Genotype (High-Level)

ΔdapA — DAP auxotroph

RP4 tra functions integrated into chromosome

recA mutation in many versions (reduces recombination)

Exact genotype varies by supplier; refer to the specific repository for official characterization.

Phenotypic Features

DAP Auxotrophy: Cannot grow without supplemented diaminopimelic acid

Efficient Donor Capability: Commonly used for mobilizing plasmids into diverse bacteria

Non-pathogenic Laboratory Strain

Biosafety

Risk Group: RG1 (typical lab E. coli K-12 derivatives), though local regulations may vary

Safe for standard molecular biology use

DAP requirement limits survival outside controlled environments

Applications

Conjugative plasmid transfer into:

Vibrio species

Pseudomonas

Shewanella

Many other Gram-negative bacteria

Used in microbial genetics and synthetic biology workflows

Quality Control (from repositories)

Genotype verification

Antibiotic resistance verification (varies with plasmid carried at distribution time)

Growth dependence on DAP

Standard sterility QC

Notes

This strain is frequently used with mobilizable plasmids that contain:

oriT (RP4 origin of transfer)
but lacks the ability to self-transfer without donor machinery.

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
電話:400-800-2947
工作QQ/微信同號:1843439339
網(wǎng)址 www.nedfriskphoto.com


Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com