BioVector? NTCC? EGFP-Plekhh1真核表達(dá)質(zhì)粒BioVector? plasmid

General Information

Plasmid Name: EGFP-Plekhh1 BioVector?

Type: Mammalian expression plasmid

Insert: Plekhh1 (Pleckstrin homology domain–containing family H member 1)

Tag: EGFP fused to Plekhh1, usually at the N-terminus for live-cell visualization

Typical Purpose:

Investigation of Plekhh1 localization

Cytoskeletal or membrane signaling studies

Overexpression studies in mammalian cells

Backbone Features (High-Level)

(General characteristics of common EGFP expression vectors; details may vary depending on the exact construct source.)

Promoter: Strong mammalian promoter (commonly CMV or similar) for high-level expression

Fluorescent Reporter: Enhanced Green Fluorescent Protein (EGFP) for visualization

Polyadenylation Signal: Typically SV40 or similar

Multiple Cloning Site (MCS): Used to insert the Plekhh1 ORF

Origin of Replication: High-copy bacterial origin (e.g., pUC ori) for propagation in E. coli

Antibiotic Selection (Bacteria): Usually ampicillin or kanamycin

Mammalian Selection (if included): Some variants include markers like neomycin, puromycin, or none (depends on specific construct source)

Insert Information: Plekhh1

Gene: Pleckstrin homology domain-containing family H member 1

Protein Domains:

PH domain

Coiled-coil regions

Possible Rho-GTPase–associated domains

Biological Roles (research context):

Cytoskeletal organization

Apical/planar cell polarity

Signal transduction pathways
(These are research associations, not functional claims.)

Expression Characteristics

Designed for fluorescent visualization of Plekhh1 dynamics

Localizes depending on Plekhh1’s natural context (often associated with membrane or cytoskeletal complexes)

Suitable for imaging using standard GFP filter sets

Applications

Subcellular localization studies

Protein–protein interaction screening (non-procedural)

Live-cell imaging of Plekhh1 distribution

Exploratory research on PH-domain proteins

Storage & Handling (High-Level)

DNA is generally stored frozen by vendors

Propagated in standard cloning strains of E. coli

Follow vendor or institutional biosafety rules for recombinant DNA

基本信息

名稱： BioVector? EGFP-Plekhh1 表達(dá)質(zhì)粒

類型： 真核表達(dá)載體

標(biāo)簽蛋白： EGFP（增強型綠色熒光蛋白）

插入基因： Plekhh1（Pleckstrin Homology Domain Containing Family H Member 1）

用途： 在哺乳動物細(xì)胞中表達(dá)帶有 EGFP 標(biāo)簽的 Plekhh1 蛋白，用于定位、蛋白相互作用研究、細(xì)胞動力學(xué)觀察等。

載體特征（高層次信息）

啟動子： 常見 CMV 或同類強啟動子（取決于具體構(gòu)建來源）

報告基因： EGFP 與 Plekhh1 融合

抗性標(biāo)記（細(xì)胞選擇）： 常見為嘌呤霉素或新霉素（以供應(yīng)商說明為準(zhǔn)）

抗性標(biāo)記（細(xì)菌）： 典型為氨芐青霉素

復(fù)制子： 常見 ColE1 類型（取決于構(gòu)建背景）

應(yīng)用方向

研究 Plekhh1 在細(xì)胞骨架動力學(xué)中的作用

觀察 Plekhh1 的亞細(xì)胞定位

蛋白質(zhì)–蛋白質(zhì)相互作用研究

分子機制研究、信號通路分析

熒光示蹤實驗

熒光特點（EGFP 標(biāo)簽）

激發(fā)峰：約 488 nm

發(fā)射峰：約 509 nm

可用于實時成像、共聚焦成像、篩選 EGFP 陽性細(xì)胞群體

儲存與注意事項（無操作步驟）

質(zhì)粒應(yīng)在供應(yīng)商要求的條件下保存（通常為超低溫）

使用前請核對序列結(jié)構(gòu)、標(biāo)簽位置、選擇標(biāo)記等

實驗中應(yīng)遵循生物安全規(guī)范并在適當(dāng)?shù)燃墝嶒炇覂?nèi)操作

質(zhì)量控制
序列驗證（Sanger 或 NGS）

質(zhì)粒純度檢測

抗性標(biāo)記驗證

插入片段完整性檢測

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
電話:400-800-2947
工作QQ/微信同號:1843439339
網(wǎng)址 www.nedfriskphoto.com


Supplier來源:BioVector NTCC Inc.
Email: [email protected]
Website網(wǎng)址: http://www.nedfriskphoto.com