BioVector? NTCC? D2SC/1永生化的小鼠脾臟樹突狀細(xì)胞系immortalized mouse dendritic cell line BioVector NTCC典型培養(yǎng)物保藏中心

BioVector? D2SC/1細(xì)胞系是一種永生化的小鼠樹突狀細(xì)胞系，從BALB/c小鼠的脾臟中分離而來。它被用作研究樹突狀細(xì)胞生物學(xué)的模型，尤其是在免疫學(xué)、抗原呈遞和免疫治療領(lǐng)域。
主要特征
來源：該細(xì)胞系來源于BALB/c小鼠脾臟中分離出的樹突狀細(xì)胞（DC）。原代細(xì)胞通過禽類逆轉(zhuǎn)錄病毒MH2的v-Myc癌基因?qū)崿F(xiàn)永生化。
抗原呈遞：D2SC/1細(xì)胞表達(dá)MHC I類和MHC II類分子，以及協(xié)同刺激分子B7-1和B7-2。這使它們能夠作為有效的抗原呈遞細(xì)胞（APC），刺激T細(xì)胞反應(yīng)。
免疫調(diào)節(jié)研究：該細(xì)胞系常用于研究β-葡聚糖或病毒蛋白等不同因素如何調(diào)節(jié)免疫應(yīng)答。相關(guān)研究包括癌癥免疫治療，研究人員通過基因改造使其呈遞腫瘤相關(guān)抗原。
培養(yǎng)條件：D2SC/1細(xì)胞通常在添加胎牛血清的杜氏改良伊格爾培養(yǎng)基（DMEM）中培養(yǎng)。它們可以作為貼壁單層細(xì)胞生長。
研究應(yīng)用
癌癥免疫治療：用于開發(fā)和測試細(xì)胞疫苗。例如，一些研究使用D2SC/1細(xì)胞與腫瘤細(xì)胞的融合雜交體，以產(chǎn)生有效的抗腫瘤免疫應(yīng)答。
抗原處理：研究樹突狀細(xì)胞如何處理外源性和內(nèi)源性抗原并將其呈遞給T細(xì)胞。
炎癥信號：研究各種刺激對DC成熟及其后續(xù)T細(xì)胞激活的影響。
基因改造：該細(xì)胞系易于進(jìn)行基因改造，使研究人員能夠探索特定基因在免疫調(diào)節(jié)中的功能。例如，研究人員創(chuàng)建了一個D2SC/1-CTLA4-Ig變體細(xì)胞系，用于研究移植模型中的同種異體移植物存活。

BioVector? D2SC/1 cell line is an immortalized mouse dendritic cell line that was isolated from the spleen of a BALB/c mouse. It is used as a model for studying dendritic cell biology, particularly in the context of immunology, antigen presentation, and immunotherapy.
Key characteristics
Origin: Derived from dendritic cells (DC) isolated from the spleen of a BALB/c mouse. The primary cells were immortalized using the avian retrovirus MH2 v-Myc oncogene.
Antigen presentation: D2SC/1 cells express both MHC Class I and MHC Class II molecules, along with the co-stimulatory molecules B7-1 and B7-2. This allows them to act as potent antigen-presenting cells (APCs) to stimulate T-cell responses.
Immunomodulatory studies: The cell line is frequently used to study how different factors, such as β-glucans or viral proteins, can modulate the immune response. This has included work related to cancer immunotherapy, where they have been genetically modified to present tumor-associated antigens.
Culture conditions: D2SC/1 cells are typically cultured in Dulbecco's modified Eagle medium (DMEM) and supplemented with fetal calf serum. They can grow as an adherent monolayer.
Research applications
Cancer immunotherapy: Used to develop and test cellular vaccines. For instance, studies have used fusion hybrids of D2SC/1 cells with tumor cells to create potent anti-tumor immune responses.
Antigen processing: Investigating how dendritic cells process both exogenous and endogenous antigens for presentation to T-cells.
Inflammatory signaling: Studying the effect of various stimuli on DC maturation and their subsequent activation of T-cells.
Gene modification: The cell line is amenable to genetic modification, allowing researchers to explore the function of specific genes in immune regulation. For example, a variant cell line, D2SC/1-CTLA4-Ig, was created to study allograft survival in transplantation models.

Supplier來源:BioVector NTCC Inc.
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